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Storage and treatment method for immunoglobulin (IgG)-coated enzyme designation strip

An immunoglobulin and processing method technology, applied in the field of food testing, can solve problems such as high cost of outsourcing, long testing time, and inability to store

Inactive Publication Date: 2014-04-02
镇江出入境检验检疫局检验检疫综合技术中心
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Since it is often necessary to apply the enzyme label strips coated with immunoglobulin, due to the high cost of outsourcing, it is necessary to make it yourself, but if the self-made enzyme label strips are not used at the moment, they cannot be stored, so they need to be prepared before each test. Enzyme-labeled strips, resulting in a longer detection time

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  • Storage and treatment method for immunoglobulin (IgG)-coated enzyme designation strip
  • Storage and treatment method for immunoglobulin (IgG)-coated enzyme designation strip
  • Storage and treatment method for immunoglobulin (IgG)-coated enzyme designation strip

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Embodiment Construction

[0010] The method for preserving and treating enzyme-labeled strips coated with immunoglobulin IgG of the present invention will be specifically described in conjunction with examples. The reagents involved in the present invention are all analytically pure.

[0011] The preparation of specific antibody immunoglobulin IgG is a well-known technique in the art. Specific antiserum was obtained by immunizing animals with an immunogen (complete antigen), and the specific antibody immunoglobulin IgG was obtained by separating and purifying the serum, and stored at -20°C for subsequent trials. In this embodiment, the pesticide metalaxyl is taken as an example. Since metalaxyl is a small molecule, it needs to be combined with a carrier protein to become an immunogen. The carrier protein is usually bovine serum albumin or ovalbumin. Use the prepared immunogen to immunize rabbits, immunize four times, and last for about 4 months to obtain specific serum, separate the specific serum, a...

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Abstract

The invention provides a storage and treatment method for an immunoglobulin (IgG)-coated enzyme designation strip. The storage and treatment method comprises the following steps: preparing a treating liquid: taking 1L of a 0.01mol / L phosphate buffered solution with a pH value being 7.2, and heating to boiling state and keeping boiling state for 3 minutes; secondly, cooling to room temperature; then sequentially adding 10g-40g of bovine serum albumin and 10g-40g of ovalbumin (OVA); agitating and mixing uniformly; adding 0.010g-0.040g of sodium azide and 0.1g-0.60g of merthiolate sodium salt in sequence, and uniformly stirring and mixing, so as to obtain the treating liquid to treat the immunoglobulin (IgG)-coated enzyme designation strip. The enzyme designation strip treated by the treating liquid has the advantages of low preparation cost and long storage life.

Description

technical field [0001] The invention relates to pesticide immunodetection technology, especially applied in the field of food detection to the detection of pesticide residue components. When detecting pesticide residues by ELISA, the enzyme-labeled immunoglobulin (IgG) strip for ELISA needs to remain active. The present invention specifically relates to a preservation and treatment method of coated immunoglobulinase labeling strips. Background technique [0002] ELISA detection technology is a new technology developed in recent decades, the core of which is the antigen-antibody binding reaction. In the process of direct competitive enzyme-linked immunosorbent assay (ELISA, Enzyme-linked immunosorbent assay), it is necessary to coat specific antibodies (IgG, Immunoglobulin, immunoglobulin) on enzyme-labeled strips, and then perform related experiments. Since immunoglobulin is an active protein, if it is not properly processed after being coated on the enzyme-labeled strip, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6854G01N33/543
Inventor 周洪斌
Owner 镇江出入境检验检疫局检验检疫综合技术中心
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