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Method of three-dimensionally preventing and controlling tomato bacterial wilt

A tomato bacterial wilt, three-dimensional technology, applied in fertilization methods, horticultural methods, botanical equipment and methods, etc., can solve problems such as low bacterial wilt effect, reduce environmental pollution, reduce investment in fixed assets, and be easy to operate. Effect

Inactive Publication Date: 2014-04-09
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Facts have proved that only relying on the root control without taking corresponding measures against the infected stems makes the effect of this method on the control of bacterial wilt extremely low, and the majority of farmers urgently need a new and effective control strategy

Method used

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  • Method of three-dimensionally preventing and controlling tomato bacterial wilt
  • Method of three-dimensionally preventing and controlling tomato bacterial wilt
  • Method of three-dimensionally preventing and controlling tomato bacterial wilt

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Embodiment Construction

[0030] (1) Isolation and identification of strains

[0031]Antagonist QL-18 for controlling continuous-cropping tomato bacterial wilt was isolated from the rhizosphere soil of healthy tomato plants in fields where the continuous-cropping tomato bacterial wilt occurred. By collecting the plants and the surrounding soil that have experienced continuous cropping obstacles and tomato bacterial wilt symptoms, the samples are stored at low temperature, and the pathogenic strains are obtained by using the SMSA bacterial wilt selective medium, and the NA-improved medium, PDA-improved medium and LB-improved Antagonism test was carried out on the culture medium by the confrontation method. Antagonistic strains were first screened, and high-efficiency antagonistic strains were re-screened through pot experiments. The genus and species were identified, and the strains were freeze-dried and stored at -20°C.

[0032] The strain QL-18 belongs to Bacillus amyloliquefaciens, and its main biolo...

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Abstract

The invention belongs to agricultural intensive production techniques and discloses a method of three-dimensionally preventing and controlling tomato bacterial wilt. The method includes: applying microbial organic fertilizer, containing antagonist QL-18, to the roots during a tomato seedling phase, and injecting NJQL-A6 agent to stems of the tomato before transplanting. The method has low cost and production process is simple; the antagonist QL-18 colonizes at the roots of the tomato in quantity, competes with bacterial wilt pathogen for root sites and releases antibiotic substance to suppress growth and augmentation of the bacterial wilt pathogen; the NJQL-A6 agent breeds and augments at the stems of the tomato, competes with the bacterial wilt pathogen for nutrition, and suppresses growth of the bacterial wilt pathogen; potting and field tests show that the efficiency of controlling tomato bacteria wilt by injecting the NJQL-A6 vaccine to the steps and applying the QL-18 containing microbial organic fertilizer to the roots is insusceptible to seasons and is stably kept above 70%.

Description

technical field [0001] The invention belongs to the agricultural intensive production technology, relates to a three-dimensional method for preventing and controlling tomato bacterial wilt, and aims to improve the efficiency of biological prevention and control of tomato bacterial wilt. Background technique [0002] Tomato bacterial wilt is a bacterial soil-borne disease of vascular bundles caused by Ralstonia solanacearum (hereinafter referred to as R. solanacearum). In my country, tomato bacterial wilt mainly occurs in the warm and humid south. With the warming of the climate and the development of high-efficiency agriculture, bacterial wilt gradually spreads to the north of my country. Ralstonia solanacearum can spread through soil, running water (irrigation, etc.), seeds, etc., and has a strong adaptability to the environment. After R. solanacearum exists in the soil, due to the small number of pathogens in the early stage, the growth of the plants is limited, the plant...

Claims

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Application Information

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IPC IPC(8): A01G13/00A01G1/00A01G7/06A01C21/00
Inventor 徐阳春韦中亚历山大·如赛谷益安
Owner NANJING AGRICULTURAL UNIVERSITY
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