In-vitro rapid propagation method for plantains

A plantain, in vitro technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as long breeding cycle, difference in medicinal value, excessive pesticide residues, etc., and achieve the effect of meeting market demand

Active Publication Date: 2014-04-09
CHANGSHA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to long-term over-harvesting, many shortcomings such as the gradual decrease of wild plantain, seeds carrying viruses, differences in medicinal value due to deterioration of environme

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The invention is used to carry out rapid propagation of plantain in vitro.

[0027] Selection and disinfection of explants: Pick wild plantain leaves free from diseases and insect pests from the wild, wash away the dust under running water, soak them in alcohol for 30s on the ultra-clean workbench, and then transfer them to 0.1% mercuric chloride solution for soaking After 8 minutes, rinse with sterile water for 3-5 times, and finally cut off the veins and edges of the plantain leaves under aseptic conditions, and cut them into small pieces of 0.8mm×0.8mm.

[0028] 1) Callus induction culture: 0.8 mm×0.8 mm small pieces cut from sterilized plantain leaves were inoculated on the callus induction medium for routine culture. The callus induction medium is based on MS medium as the basic medium, and the contained sucrose concentration is 30g / L, the concentration of naphthaleneacetic acid is 0.1mg / L, and the concentration of 6-benzylaminopurine is 1.0mg / L. , the medium pH w...

Embodiment 2

[0032] The invention is used to carry out rapid propagation of plantain in vitro.

[0033] The callus induction medium is based on MS medium as the basic medium, and the contained sucrose concentration is 30g / L, the concentration of naphthaleneacetic acid is 0.1mg / L, and the concentration of 6-benzylaminopurine is 2.0mg / L. , the medium pH was 6.0. Other conditions are with embodiment 1.

[0034] The results showed that the disinfection efficiency of the explants was as high as 80%, the aseptic adventitious buds could be obtained quickly, the rooting rate was 100%, and a large number of plantain seedlings maintaining the excellent traits of the mother plant could be obtained.

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Abstract

The invention provides an in-vitro rapid propagation method for plantains. The in-vitro rapid propagation method for the plantains comprises the following steps: (1) callus induction culture, namely cutting off leaf veins and leaf margins of sterilized and disinfected plantains and cutting into small blocks; inoculating the plantains to a callus induction culture medium; and carrying out regular culture to form calluses and adventitious buds; and (2) rooting culture, namely selecting the adventitious buds, with the bud lengths of not more than 2cm-3cm, obtained in the step (1); cutting off the adventitious buds from the calluses and continuing to carry out callus induction culture on the residual calluses and the adventitious buds; and inoculating the cut adventitious buds on a rooting culture medium; and regularly culturing until white roots grow to obtain aseptic plantain germchits. According to the method provided by the invention, the disinfection efficiency of explants is up to 80% and the aseptic seedling rooting rate is 100%; the aseptic plantain germchits can be rapidly propagated; a plurality of problems that the propagation capability of plantain plants is low, the propagation period is long, resources are lacked and a lot of pesticides are remained are solved; the in-vitro rapid propagation method for the plantains has very important meanings in the aspects of protecting the natural resources of the wild plantains, protecting a good ecological environment and the like.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, and in particular relates to a method for in vitro rapid propagation of plantain. Background technique [0002] Plantain belongs to the plantain family plant, also known as sweet grass, plantain, wheel dish, pig ear grass, etc. It grows in humid environments, such as ponds, under trees, by rivers, roadsides and other places. Plantain has edible and therapeutic value, is rich in various vitamins and proteins, and contains rich and excellent soluble dietary fiber. [0003] Both the seeds and the whole herb of plantain can be used as medicine, and have less toxic and side effects. Diuretic and swelling, antidiarrheal, heat-clearing and sterilizing, eyesight and phlegm resolving, anti-inflammatory, lowering blood pressure and other effects; the three ingredients contained in β-epiloganic acid (β-epiloganic acid), carotene and luteolin can not only eliminate Oxygen free radicals in the body preve...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陈建荣唐映红刘芳郭清泉
Owner CHANGSHA UNIVERSITY
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