Preparation method and application of turtle shell active polypeptide extractive

A technology of active polypeptides and extracts, which is applied in the field of preparation of active polypeptide extracts of soft-shelled turtle shells, to achieve the effect of good hepatoprotection, high separation efficiency, and easy operation

Active Publication Date: 2014-04-09
HUBEI UNIV OF CHINESE MEDICINE
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The preparation of the anti-hepatic fibrosis active polypeptide extract of turtle shell has been reported in the literature by using ultrasonic extraction followed by semi-permeable membrane dialysis. This method is only suitable for a small amount of preparation in the laboratory.

Method used

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  • Preparation method and application of turtle shell active polypeptide extractive
  • Preparation method and application of turtle shell active polypeptide extractive
  • Preparation method and application of turtle shell active polypeptide extractive

Examples

Experimental program
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Effect test

Embodiment 1

[0030] A preparation method of turtle shell active polypeptide extract, the steps are as follows:

[0031] (1) Take the turtle shell and crush it into coarse powder, add water to decoct twice, add 4 times the weight of water each time, decoct for 3 hours each time, filter, and combine the filtrate to obtain the turtle shell extract;

[0032] (2) Dilute the turtle shell extract with distilled water to a concentration equivalent to 4g / 100mL of the turtle shell medicinal material, and then filter it with a microfiltration membrane with a pore size of 0.3 μm to obtain a microfiltrate;

[0033] (3) The microfiltrate passes through an ultrafiltration membrane with a molecular weight cut-off of 15KD under a pressure of 0.3Mpa, collects the permeate, and discards the retentate;

[0034] (4) Pass the permeate obtained in step (3) through an ultrafiltration membrane with a molecular weight cut-off of 6KD under a pressure of 1.5Mpa, and collect the permeate and retentate separately;

[...

Embodiment 2

[0039] A preparation method of turtle shell active polypeptide extract, the steps are as follows:

[0040] (1) Take the turtle shell and crush it into a coarse powder, add water to decoct 3 times, add 6 times the weight of water each time, decoct for 3 hours each time, filter, and combine the filtrate to obtain the turtle shell extract;

[0041] (2) Dilute the turtle shell extract with distilled water to a concentration equivalent to 6g / 100mL of the turtle shell medicinal material, and then filter it with a microfiltration membrane with a pore size of 0.3 μm to obtain a microfiltrate;

[0042] (3) The microfiltrate passes through an ultrafiltration membrane with a molecular weight cut-off of 20KD under a pressure of 0.2Mpa, collects the permeate, and discards the retentate;

[0043] (4) Pass the permeate obtained in step (3) through an ultrafiltration membrane with a molecular weight cut-off of 8KD under a pressure of 0.8Mpa, and collect the permeate and retentate separately; ...

Embodiment 3

[0048] A preparation method of turtle shell active polypeptide extract, the steps are as follows:

[0049] (1) Take the turtle shell and crush it into coarse powder, add water to decoct 3 times, add 8 times the weight of water each time, decoct for 2 hours each time, filter, and combine the filtrate to obtain the turtle shell extract;

[0050] (2) Dilute the turtle shell extract with distilled water to a concentration equivalent to 8 g / 100 mL of the turtle shell medicinal material, and then filter it with a microfiltration membrane with a pore size of 0.3 μm to obtain a microfiltrate;

[0051] (3) The microfiltrate passes through an ultrafiltration membrane with a molecular weight cut-off of 25KD under a pressure of 0.15Mpa, collects the permeate, and discards the retentate;

[0052](4) Pass the permeate obtained in step (3) through an ultrafiltration membrane with a molecular weight cut-off of 10KD under a pressure of 0.5Mpa, and collect the permeate and retentate separately;...

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Abstract

The invention belongs to the technical field of preparation of traditional Chinese medicine extractives, and particularly discloses a preparation method and application of a turtle shell active polypeptide extractive. The preparation method particularly comprises the following steps of decocting a turtle shell by using water for extraction; diluting an extracting solution by using distilled water till the concentration corresponding to contain 4-8 g / 100 mL of turtle shell medicinal materials; respectively carrying out microfiltration and multistage ultrafiltration to obtain ultrafiltrate, carrying out vacuum concentration, and freeze-drying to obtain freeze-dried powder with molecular weight less than 6 KD. The preparation method disclosed by the invention can be used for preparing the active polypeptide substance part of the turtle shell by adopting a multistage membrane separation method, and can transit to industrialization on process parameters through a pilot scale test; the prepared turtle shell active polypeptide extractive has a liver protection effect and can be used for preparing a drug or health-care food used for preventing or treating liver fibrosis and alcoholic liver injury.

Description

technical field [0001] The invention relates to the technical field of preparation of traditional Chinese medicine extracts, in particular to a preparation method and application of a turtle shell active polypeptide extract. Background technique [0002] Turtle shell is a traditional Chinese medicine for both medicine and food. It comes from the carapace of Trionyx sinensis Wiegmann. It was first recorded in "Shen Nong's Materia Medica". Yang suppressing, softening hard mass and resolving stagnation, the effect of reducing fever and removing steam; mainly treating diseases such as fever due to yin deficiency, bone steam due to labor heat, lump in the abdomen, and internal movement due to deficiency. Turtle shell and its compound preparations are often used clinically to treat liver fibrosis, and have good curative effect. The chemical components of turtle shell mainly include amino acids, polypeptides, polysaccharides, trace elements, animal glue, and protein. It has been ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/00A61K35/56A23L1/29A23L1/325A61P1/16A23L17/00A23L33/00A61K35/586
Inventor 叶晓川刘焱文刘丹李水清孔菲菲李捷马丽
Owner HUBEI UNIV OF CHINESE MEDICINE
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