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Extraction method for increasing tylosin A component

A technology of tylosin and extraction method, which is applied in chemical instruments and methods, preparation of sugar derivatives, carboxylate preparation, etc., can solve problems such as abnormal fermentation of tylosin and solvent loss, and improve the ability to resist risks , Improve the purity and improve the pass rate

Active Publication Date: 2014-04-09
浙江普洛生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In view of the shortcomings and deficiencies in the above-mentioned prior art, the inventor aims to provide a method for improving the extraction of Tylosin A component, while improving the product yield, on the one hand, it solves the problem of solvent loss, and on the other hand, it solves the problem of Tylosin A. The problem of abnormal fermentation of bacteria

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Extraction of Tylosin with Toluene-Butyl Acetate Composite Solvent with 95% Toluene Content (V / V)

[0024] A method for improving the extraction of Tylosin A component, comprising the steps of:

[0025] Tylosin fermentation filtrate 1000ml, titer 9865μ / ml. Use 15% sodium hydroxide solution to adjust the pH value to 9.0-9.5, heat to 40°C, add 300ml of toluene-butyl acetate composite solvent with 95% toluene content (V / V) for extraction; after standing for stratification, the lower aqueous phase Extract and layer again with 300ml of toluene-butyl acetate composite solvent, and combine the two composite solvent extracts. Cool the extract to below 15°C, slowly add tartaric acid solution with a mass concentration of 2.5% under the condition of heat preservation for back extraction, control the pH value of the end point of the back extraction to be 3.7~4.2, and stand to separate to obtain the back extraction solution. Adjust the pH value of the back extract to 6....

Embodiment 2

[0028] Example 2 Extraction of Tylosin with Toluene-Butyl Acetate Composite Solvent with Toluene Content 70% (V / V)

[0029]Tylosin fermentation filtrate 1000ml, titer 101325μ / ml. Use 15% sodium hydroxide solution to adjust the pH value to 9.0-9.5, heat to 30°C, add 350ml of toluene-butyl acetate composite solvent with 70% toluene content (V / V) for extraction; after standing for stratification, the lower aqueous phase Again use 350ml of toluene-butyl acetate composite solvent for extraction and layering, and combine the two composite solvent extracts. Cool the extract to below 10°C, slowly add a phosphoric acid solution with a mass concentration of 2.0% under the condition of heat preservation for back extraction, control the pH value at the end of the back extraction to 3.7~4.2, and stand to separate to obtain the back extraction. Adjust the pH value of the back extract to 6.5 with 15% calcium hydroxide suspension, add 0.5% activated carbon to decolorize for 1 hour, and vac...

Embodiment 3

[0032] Example 3 Treat the ferment filtrate of component C with incompletely converted tylosin with a toluene-butyl acetate composite solvent with a toluene content of 80% (V / V)

[0033] Tylosin fermentation filtrate 1000ml, titer 9361μ / ml. Use 15% sodium hydroxide solution to adjust the pH value to 9.0-9.5, heat to 50°C, add 350ml of toluene-butyl acetate composite solvent with 80% toluene content (V / V) for extraction; after standing and stratifying, the lower aqueous phase Again use 350ml of toluene-butyl acetate composite solvent for extraction and layering, and combine the two composite solvent extracts. Cool the extract to below 15°C, slowly add tartaric acid solution with a mass concentration of 2.5% under the condition of heat preservation for back extraction, control the pH value of the end point of the back extraction to be 3.7~4.2, and stand to separate to obtain the back extraction solution. Adjust the pH value of the back extract to 6.5 with 15% calcium hydrox...

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PUM

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Abstract

The present invention relates to a tylosin extraction technology, and specifically discloses an extraction method for increasing a tylosin A component, wherein a composite solvent is adopted to extract tylosin from a tylosin fermentation filtrate, the composite solvent comprises toluene and butyl acetate or isobutyl acetate, and the proportion of toluene in the composite solvent is 70-95% (V / V). According to the extraction method, the product yield is increased, the solvent loss problem is solved, and the abnormal tylosin fermentation problem is solved.

Description

technical field [0001] The invention belongs to the field of antibiotic drugs, and in particular relates to the extraction technology of tylosin, which can effectively improve the A component in the tylosin product. Background technique [0002] Tylosin is a 16-membered lactone special antibiotic for poultry and livestock. It is a mixture of four components A, B, C and D obtained from Streptomyces fradiae during the fermentation process. : A component is Tylosin, B component is Desmycosin, C component is Macrosin, D component is Relomycin ) and the structural analogue laktin. Among the above four components, all the components except resamectin have antibacterial activity in test tubes, and the tylosin A component has been confirmed to have higher activity in vivo. Common tylosin products are tylosin tartrate, tylosin phosphate and tylosin base. Tylosin is effective against Gram-positive bacteria and certain negative bacteria (such as Staphylococcus aureus, Streptococcus py...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/08C07H1/08C07C59/255C07C51/41
Inventor 许永兴胡露华周晋武金康康
Owner 浙江普洛生物科技有限公司
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