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Method for extracting tea polyphenol from tea leaves by combination of metal ion complexing precipitation and column chromatography

A technology of complexation precipitation and metal ions, which is applied in the fields of application, drug combination, and pharmaceutical formula, can solve the problems of large amount of organic solvents, low active ingredients, and toxic solvents, etc., and achieve high extraction rate and good activity retention , the effect of mild conditions

Inactive Publication Date: 2014-04-16
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can solve the problems of low extraction rate, low active ingredient, large amount of organic solvents, and toxic solvents in the existing technology, reduce production costs, and realize industrialized production and other advantages

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method for extracting tea polyphenols from tea leaves by combining metal ion complex precipitation and column chromatography, comprising the following steps:

[0030] (1) Selection and testing of raw materials

[0031] The tea raw materials were purchased from the tea farmer’s market in Hefei, Anhui. According to the method of GB / T 8313-2008, the content of tea polyphenols in this batch of tea was found to be 24.05%;

[0032] (2) Extract 100g of tea raw material and grind it to 30 mesh, add deionized water at a temperature of 90°C, stir and reflux extraction, 30min / time, leaching twice (1200g deionized water for the first time, 1200g deionized water for the second time), Filtration and merging of tea extracts;

[0033] (3) Precipitation Press ZnCl 2 Add 20% ZnCl to the extract at a mass ratio of 3:1 to tea polyphenols 2 solution, then adjust the pH to 6.0 with 10% sodium bicarbonate solution, centrifuge after 40 minutes of precipitation, and separate the...

Embodiment 2

[0043] A method for extracting tea polyphenols from tea leaves by combining metal ion complex precipitation and column chromatography, comprising the following steps:

[0044] (1) Selection and testing of raw materials

[0045] The tea raw materials were purchased from the tea farmer’s market in Hefei, Anhui Province. According to the method of GB / T 8313-2008, the content of tea polyphenols in this batch of tea leaves was 25.11%;

[0046] (2) Extract 500g of tea raw material, grind it to 30 mesh, add deionized water, ultrasonically extract at 90°C, stir and reflux extraction, 30min / time, extract twice (6000g deionized water for the first time, 5000g for the second time) deionized water), filtered to obtain tea extract;

[0047] (3) Precipitation Add 20% ZnCl to the extract according to the mass ratio of ZnCl and tea polyphenols of 2:1 2 solution, then adjust the pH to 6.5 with 10% sodium bicarbonate solution, centrifuge after 60 minutes of precipitation, and separate the...

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PUM

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Abstract

The invention provides a method for extracting tea polyphenol from tea leaves by combination of metal ion complexing precipitation and column chromatography. The whole process is in an acidic environment, and the method comprises the following steps: stirring crushed tea leaves with deionized water, and performing reflux extraction; performing acidolysis precipitation on the extract by adopting metal ion complexing precipitation; extracting with ethyl acetate; concentrating the extract in vacuum to obtain a crude product tea polyphenol; performing polyamide resin column chromatography under a faintly acid condition to obtain pure tea polyphenol. The method is mild in condition, can furthest reduce loss of effective components, avoids using chloroform and other toxic organic solvents; the extraction rate of tea polyphenol can be over 70 percent, and the purity of tea polyphenol can be over 98 percent.

Description

technical field [0001] The invention relates to the field of tea extraction, in particular to a method for extracting tea polyphenols from tea leaves by combining metal ion complex precipitation and column chromatography. technical background [0002] Tea polyphenols, also known as tea tanning or tea tannins, are one of the main components that form the color and aroma of tea, including catechins, flavonoids, anthocyanins, and phenolic acids. The content of tea polyphenols accounts for 15% to 30% of the dry matter of tea leaves, and the flavanol compounds mainly composed of catechins account for 60% to 80% of the total amount of tea polyphenols. Among them, the components with the highest content are Epigallo catechin gallate (EGCG) (50%-60%), epigallo catechin (EGC) (15%-20%), epicatechin gallate ( epicatechin gallate (ECG) (10%-15%) and epicatechin (epicatechin, EC) (5%-10%). Tea polyphenols have good antioxidant properties, and their antioxidant properties are 10 to 20 ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/82A61P35/00A61P39/06A61P3/10A61P9/00A23L3/3472A61K127/00
Inventor 袁传勋刘小芳金日生
Owner HEFEI UNIV OF TECH