Pig spleen transfer factor extracting method

A technology of pig spleen transfer factor and extraction method, applied in the field of veterinary biological products, can solve problems such as affecting process efficiency, and achieve the effects of reducing the difficulty of centrifugation, shortening the production cycle, and reducing the solid content of the feed liquid

Active Publication Date: 2014-04-16
TIANJIN RINGPU BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The solid-liquid separation operation in the prior art is generally realized by centrifugation. However, due to the high emulsification of the cell crushed material and the very uniform gas-liquid-solid three-phase mixing, the density of the solid in the material is lower than that of the liquid. Therefore, it is difficult to adjust the material by conventional centrifugation. Liquid to achieve solid-liquid separation, which in turn affects the overall process efficiency

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1) Remove about 65.0 kg of frozen pig spleen from the surface packaging, and then thaw it overnight with purified water;

[0027] 2) Rinse the spleen with purified water the next day, pick out unqualified spleens, and remove large tissues such as fascia and fat;

[0028] 3) Weigh the spleen at 6.0kg / part and grind it with a meat grinder, coarsely grind it twice;

[0029] 4) Mix every 2 parts, add 0.1 times the mass (1.2kg) of purified water, mix well, and then grind through the oblique tooth colloid mill for 2 times, and the diamond tooth colloid mill for 1 time, put it into a clean bucket, and pack it together 5 barrels; the remaining spleen was recovered and frozen;

[0030] 5) Add 1.4 times the mass (16.8kg) of purified water into each barrel, stir to make a homogenate;

[0031] 6) The homogenate is broken by a high-pressure homogenizer, homogenized once, the temperature of the slurry is controlled online at 35-40°C, and then the temperature is controlled to 23°C, ...

Embodiment 2

[0039] 1) Remove about 65.0 kg of frozen pig spleen from the surface packaging, and then thaw it overnight with purified water;

[0040] 2) Rinse the spleen with purified water the next day, pick out unqualified spleens, and remove large tissues such as fascia and fat;

[0041] 3) Weigh the spleen at 6.0kg / part and grind it with a meat grinder, coarsely grind it twice;

[0042] 4) Mix every 2 parts, add 0.25 times the mass (3kg) of purified water, mix well, and then grind it through the oblique tooth colloid mill twice, and the rhombus tooth colloid mill once, put it into a clean bucket, and pack 5 pieces in total. bucket; the remaining spleen was recovered and stored in a freezer;

[0043] 5) Add 1.15 times the mass (13.8kg) of purified water into each barrel, stir to make a homogenate;

[0044] 6) The homogenate is broken by a high-pressure homogenizer, homogenized twice, the temperature of the slurry is controlled on-line at 30-35°C, and then the temperature is controlled...

Embodiment 3

[0052] 1) Remove about 65.0 kg of frozen pig spleen from the surface packaging, and then thaw it overnight with purified water;

[0053] 2) Rinse the spleen with purified water the next day, pick out unqualified spleens, and remove large tissues such as fascia and fat;

[0054] 3) Weigh the spleen at 6.0kg / part and grind it with a meat grinder, coarsely grind it twice;

[0055] 4) Mix every 2 parts, add 0.5 times the mass (6kg) of purified water, mix well, and then grind it twice through the oblique tooth colloid mill, and grind it once through the diamond tooth colloid mill, put it into a clean storage bucket, and pack 5 pieces in total. bucket; the remaining spleen was recovered and stored in a freezer;

[0056] 5) Add 1.1 times the mass (13.2kg) of purified water into each barrel, stir to make a homogenate;

[0057] 6) The homogenate is broken through a high-pressure homogenizer, homogenized 3 times, the temperature of the slurry is controlled online at 25-30°C, and then ...

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Abstract

The invention provides a pig spleen transfer extracting method. The method has the characteristics that a high-pressure homogenizing machine is used for rapidly breaking spleen tissues and cells, a freezing step is not needed, and the production period can be more effectively shortened; the effective solid-liquid separation process is introduced after the homogenizing and before the filtering to demulsify the material and discharge gas in the material, so that the centrifugal difficulty is alleviated; the clear liquid supernatant is obtained through a centrifuging manner; the content of solids of the liquid filtered by a membrane can be obviously reduced, and the filtering rate and the service life of the membrane are increased; matched with the membrane filtering technology, the production period of the stock solution can be obviously shortened, convenience in operation is realized, the automation degree is high, and the large-scale production requirement can be met.

Description

technical field [0001] The invention relates to the field of veterinary biological products, in particular to a method for extracting pig spleen transfer factor. Background technique [0002] Transfer factor (TF for short) is a substance released by lymphocytes that can transfer sensitization information. It can specifically transfer the cellular immune information of the donor to the recipient, thereby enhancing the immune function of the recipient. It has become a widely used immune-enhancing preparation abroad. [0003] The transfer factor exists in the lymphocytes of the spleen. The transfer factor preparation method in the prior art is generally to grind the animal spleen to destroy the cell structure to release the intracellular transfer factor into the serum, and then separate and refine the transfer factor in the serum Finally, the transfer factor product is obtained. [0004] The composition of animal spleen serum is complex, so animal TF is not easy to separate a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/52C07K1/34
CPCC07K14/52
Inventor 杨燚苏建东王尚尚王甜田凤菊李建丽吴全忠杨保收李守军
Owner TIANJIN RINGPU BIO TECH
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