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Method for preparing collagen polypeptides by using larimichthys crocea leftovers

A collagen polypeptide and scrap technology, applied in the biological field, can solve the problems of wasting nutrients and polluting the environment.

Active Publication Date: 2014-04-16
浙江东杰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Collagen is also abundant in fish viscera, and its molecule has a unique triple helix structure. If it is not treated effectively, it will not only pollute the environment, but also waste a lot of nutrients

Method used

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  • Method for preparing collagen polypeptides by using larimichthys crocea leftovers

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Embodiment 1

[0016] (1) Preparation of fish waste mixture: the fish skin, fish scales, and fish viscera were processed separately to remove miscellaneous proteins and facilitate the dissolution of collagen. The fish skin is thawed, cleaned, cut into pieces, soaked in 0.1mol / L NaOH 20 times its mass for 2 hours, and cleaned for later use; the fish scales are thawed, cleaned, soaked in 0.4mol / L HCl 20 times its mass for 2 hours, and its mass is Soak in 0.1mol / L NaOH 20 times of it for 2 hours, wash it for later use; thaw, wash and mince the fish viscera for later use; then mix the separately processed fish scraps with fish skin, fish scales and viscera in a certain mass fraction ratio Obtain the fish offal mixture and mix it for subsequent use.

[0017] (2) Pick activated Bacillus coagulans from the nutrient agar slant medium, inoculate it in the nutrient broth seed medium, culture it on a shaker at 40°C for 12 hours, and then inoculate the seed solution on the nutrient meat at an inoculatio...

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Abstract

The invention discloses a method for preparing collagen polypeptides by using larimichthys crocea leftovers. The larimichthys crocea leftovers are fermented by condensed bacillus to obtain collagen polypeptide fermented substances, and the molecular weight of a product is 1,000-3,000 daltons; the larimichthys crocea leftovers comprise fish scales, fish skins and viscera mixtures; the preservation number of the condensed bacillus is CCTCC No:M2013193. According to the method, main nutritional substances produced by fermenting the larimichthys crocea leftovers by the condensed bacillus are free calcium and the collagen polypeptides; acidic products and the calcium which are produced in a fermenting process can be converted into soluble calcium; furthermore, extracellaluar proteases can be used for hydrolyzing proteins in the larimichthys crocea leftovers to supply the collagen polypeptides, ammonia acid, phosphorus and the like which assist in calcium absorption.

Description

technical field [0001] The invention relates to biotechnology, in particular to a method for preparing collagen polypeptide by fermenting fish leftovers with probiotic bacillus coagulans. Background technique [0002] In recent years, with the adjustment of my country's aquatic industry structure, the development of aquatic product processing industry has gradually grown. However, a large amount of leftovers will be produced while processing fish products, accounting for 30-50% of the total mass of processed fish. Restricted by technology and traditional concepts, fish leftovers have not been fully utilized for a long period of time, but have been thrown away as garbage. In fact, after processing and comprehensive utilization of fish waste, it can not only turn waste into treasure but also avoid polluting the environment. It is also an effective way to increase the efficiency of enterprises and the income of fish farmers. When people process or eat fish, the proportion of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/02C12R1/07
Inventor 王彦波傅玲琳李刚
Owner 浙江东杰生物科技有限公司
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