Preparation method and application of kit for double-sandwich immunofluorescence quantitative detection of human anti-Mullerian hormone (AMH) on basis of quantum dots

Abstract

The invention discloses a kit for double-sandwich immunofluorescence quantitative detection of human anti-Mullerian hormone (AMH) on the basis of quantum dots and application thereof. The original kit contains an AMH protein standard substance, an ELISA plate coated with an AMH specific polyclonal antibody and a CdTe quantum dot labeled AMH monoclonal antibody. The detection antibody used in the kit is a CdTe quantum dot label for monoclonal antibodies, and can better remove background signals; the detection method is simple and convenient, and has high practicality; the detection result directly determined by a fluorescent ELIASA indicates that the emitted fluorescence has the advantages of narrow spectrum peak, weak autofluorescence and high sensitivity; the kit has the advantages of high fluorescence intensity and long stabilization time, and overcomes the actual states of low sensitivity and poor specificity in the traditional ELISA detection kit; and the kit can enhance the resolution, sensitivity and specificity of ovary reservation function detection.

Description

technical field [0001] The invention belongs to the technical field of immunodiagnosis and relates to quantum dot-based immunofluorescence quantitative detection, in particular to a quantum dot-based double-sandwich immunofluorescence quantitative detection kit for human anti-Müllerian hormone (AMH) and its preparation and application . Background technique [0002] At present, clinical evaluation of ovarian function in various domestic clinics is mainly based on age, the number of antral follicles (Antral follicle count), follicle stimulating hormone (FSH) and estrogen (E2) levels, etc. However, the above-mentioned traditional methods are prone to errors due to the influence of various factors, and at the same time, they are prone to result bias due to the subjective judgment of the tester; in addition, the detection of the above indicators is restricted by the time limit of the menstrual cycle, and the detection value is affected by the fluctuation of the menstrual cycle. ...

AI Technical Summary

Problems solved by technology

Some domestic diagnosis and treatment institutions have introduced the kit and conducted relevant clinical tests, but its price is relatively expensive and cannot be effectively promoted clinically

At present, there is no registered kit supply using quantum dot immunoassay method to detect AMH in China, and the clinical research of this detection has not yet been widely carried out.

And at present, most foreign AMH detection kits use the enzyme-linked immunosorbent method, but the enzyme immunoassay method has low sensitivity and many influencing factors, which can easily cause false negatives and false positives

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