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Photosynthetic bacteria sc01 and its rapid culture method and application

A technology of photosynthetic bacteria and culture methods, applied in the direction of bacteria, applications, and methods based on microorganisms, can solve the problems of no systematic theoretical guidance on the timing of use, etc., achieve simple and easy screening methods, reduce residual bait rate, and obvious use effects Effect

Active Publication Date: 2016-08-17
LAIZHOU SHUNCHANG AQUATIC PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is still no systematic theoretical guidance for the selection of strains, the amount of photosynthetic bacteria used, and the timing of use, and there is still a large blindness in the process of production and use

Method used

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  • Photosynthetic bacteria sc01 and its rapid culture method and application
  • Photosynthetic bacteria sc01 and its rapid culture method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The screening of embodiment 1 photosynthetic bacteria

[0022] 1) Sampling and treatment: Take 8g of seawater from the bottom of the drainage ditch of the turbot nursery factory, pond wall attachments, sludge at the bottom of the pond, seawater at the bottom of the nursery and culture ponds, attachments, pond walls, and bottom of the pond. Add 100ml of sterilized seawater, place it in a 300ml sterile Erlenmeyer flask, shake it fully for 15 minutes, remove large particles such as weeds and rocks, and prepare a sample suspension.

[0023] 2) Enrichment culture: Take 5mL of the sample suspension and add 50mL of sterile enrichment medium (KH 2 PO 4 0.5g / L, yeast extract 1.5g / L, tryptone 0.05g, CaCl0.05g, NaAc1.0g / L, NH 4 Cl0.2g / L, MnSO 4 0.05g, MgSO 4 0.025g, FeSO 4 0.002g, pH value 7.2, dilute to 1000mL with distilled water), add 10ml paraffin oil, and seal with parafilm. 28 ℃, 2000lux light culture. Observe the color change of the culture medium every 48h. This en...

Embodiment 2

[0025] The cultivation of embodiment 2 photosynthetic bacteria SC01

[0026] The photosynthetic bacteria obtained in the above examples were rapidly cultured, and 50ul-80°C frozen SC01 was inoculated into 10ml rapid medium (K 2 HPO 4 0.25g / L, KH 2 PO 4 0.25g / L, yeast extract 3.0g / L, NaAc2.0g / L, NH 4 Cl1.0g / L, pH 6.8, dilute to 1000mL with old sea water (remove the sediment after standing for 14 days). ), 28°C, 300rpm, cultured under natural light for 120h to OD 600 to 1.0, use this as the seed solution, inoculate the seed solution in the fast medium according to the inoculum amount of 5% (volume ratio), culture at 28°C, 150rpm, and under natural light for 120h. Take out the culture medium at intervals of 2 hours to measure OD 600 . Plot the growth curve of SC01 ( figure 1 ).

[0027] Use the plate count method to calculate the colony-forming unit (CFU, colony-forming unit) per unit volume: to be SC01 bacterial solution OD 600 to 1.0, take 1m with sterile sea water ac...

Embodiment 3

[0028] The influence of embodiment 3SC01 on residual bait rate

[0029] Take OD 600 5ml of SC01 culture solution to 1.0 was added to the holding pond of 50L seawater, and 10 healthy turbot with a total body length of 6-8cm were put into each breeding pond. Under normal indoor light, the aeration rate of the breeding pond is 6.5L per minute, and the amount of conventional compound granular bait for farming (the granular bait usually does not disintegrate in seawater for 2 hours) is based on 300 grains (about 10g) of granular bait per pond per day. Add 3 times. 2 hours after each feeding, the residual bait in the culture ponds was fished out, the quantity of the residual bait in each culture pond was counted, and the residual bait rate was calculated. At the same time, taking the temporary culture pond without adding SC01 culture medium as a control, it was observed that in the culture ponds added with photosynthetic bacteria SC01, turbot had a strong desire to eat and compete...

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Abstract

The invention relates to cultivation of microorganisms, and particularly relates to photosynthetic bacteria SC01 as well as a cultivation method and an application thereof. The photosynthetic bacteria are Rhodobacter sphaeroides, and preserved in China Microbiological Collection Center on September 25, 2013, with the preservation number of CGMCC No.8248. The photosynthetic bacteria SC01 are used in a cultivation process of breeding organisms as an additive so as to increase the food intake of breeding organisms, and reduce the residual feed rate. The bacterial strain provided by the invention has the characteristics that a screening method is easy and feasible, the production, storage and transportation are convenient, the using effect is obvious and the like, and has good application prospect.

Description

technical field [0001] The invention relates to the cultivation of microorganisms, in particular to a photosynthetic bacterium SC01 and its rapid cultivation method and application. Background technique [0002] After decades of rapid development, my country's mariculture industry has played a pivotal role in the development of the coastal economy and even the national economy. The species of mariculture include fish, shrimp, crab, shellfish, sea cucumber and other major economic species. Shandong is a major province in my country's mariculture industry. Taking sea cucumber farming as an example, in 2012, the sea cucumber farming area in Shandong Province reached 800,000 mu, with an annual output of 71,000 tons, accounting for more than half of the country's total sea cucumber production, with an annual output value of 16 billion yuan. And develop at an annual growth rate of 20%. [0003] With the rapid development of the marine aquaculture industry, the problems of disease...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K20/00C12R1/01
Inventor 栾顺香焦绪栋秦显明
Owner LAIZHOU SHUNCHANG AQUATIC PROD
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