Schizochytrium limacinum fermentation medium
A fermentation medium, technology of Schizochytrium, applied in fermentation, microorganism-based methods, microorganisms, etc., can solve the problems of shortening the service life of equipment, seriousness, corrosion, etc., to reduce investment in fixed assets, promote synthesis, and promote growth Effect
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Embodiment 1
[0012] (1) Add 50mL seed medium (glucose 50.0g / L, yeast extract 5.0g / L, peptone 3.0g / L, MgSO 4 2.0g / L, KH 2 PO 4 2.0g / L), the inoculum amount was 4%, and cultured for 2 days at a culture temperature of 25°C and a shaker speed of 180rpm;
[0013] (2) Add 50mL of fermentation medium into a 250mL Erlenmeyer flask, and inoculate Schizochytrium into the fermentation medium at an inoculum of 4%;
[0014] Fermentation medium components:
[0015] Ⅰ. Glucose 100.0g / L, yeast extract 15.0g / L, MgSO 4 1.0g / L, KH 2 PO 4 3.0g / L, NaNO 3 4.0g / L, Na 2 SO 4 12.0g / L;
[0016] Ⅱ. Glucose 100.0g / L, yeast extract 15.0g / L, MgSO 4 1.0g / L, KH 2 PO 4 3.0g / L, NaNO 3 12.0g / L, Na 2 SO 4 5.0g / L;
[0017] Ⅲ. Glucose 100.0g / L, yeast extract 15.0g / L, MgSO 4 1.0g / L, KH 2 PO 4 3.0g / L, NaNO 3 9.0g / L, Na 2 SO 4 8.0g / L;
[0018] CK: glucose 100.0g / L, yeast extract 15.0g / L, MgSO 4 1.0g / L, KH 2 PO 4 3.0g / L, NaNO 3 4.0g / L, sea crystal 10.0g / L, other culture conditions are the same as above; ...
Embodiment 2
[0025] (1) Add 50mL seed medium (glucose 50.0g / L, yeast extract 5.0g / L, peptone 3.0g / L, MgSO 4 2.0g / L, KH 2 PO 4 2.0g / L), the inoculum amount was 4%, and cultured for 2 days at a culture temperature of 25°C and a shaker speed of 180rpm;
[0026] (2) Add 50mL of fermentation medium into a 250mL Erlenmeyer flask, and inoculate Schizochytrium into the fermentation medium at an inoculum of 4%;
[0027] Fermentation medium components:
[0028] Ⅰ. Glucose 100.0g / L, yeast extract 15.0g / L, MgSO 4 1.0g / L, KH 2 PO 4 3.0g / L, NaNO 3 7.0g / L, K 2 SO 4 2.0g / L, Na 2 SO 4 8.0g / L, KNO 3 3.0g / L; conductivity 27.22ms;
[0029] Ⅱ. Glucose 100.0g / L, yeast extract 15.0g / L, MgSO 4 1.0g / L, KH 2 PO 4 3.0g / L, NaNO 3 6.0g / L, K 2 SO4 3.0g / L, Na 2 SO 4 5.0g / L, (NH 4 ) 2 SO 4 1.0g / L; conductivity 27.47ms;
[0030] Ⅲ. Glucose 100g / L, yeast extract 15.0g / L, MgSO 4 1.0g / L, KH 2 PO 4 3.0g / L, NaNO 3 4.0g / L, K 2 SO 4 5.0g / L, Na 2 SO 4 12.0g / L, KNO 3 5.0g / L, (NH 4 ) 2 SO 4 3.0g / L...
Embodiment 3
[0038] (1) Preparation of seed medium: glucose 50.0g / L, yeast extract 5.0g / L, peptone 3.0g / L, MgSO 4 2.0g / L, KH 2 PO 4 2.0g / L; the initial pH value is 6.0, sterilized at 121°C for 30min, cooled for later use;
[0039] (2) Preparation of fermentation medium: glucose 100g / L, yeast extract 15.0g / L, MgSO 4 1.0g / L, KH 2 PO 4 3.0g / L, NaNO 3 12.0g / L, Na 2 SO 4 5.0g / L; the initial pH value is 6.0, sterilized at 121°C for 30min, cooled for later use;
[0040] (3) Take 10mL-20°C freezer-stored Schizochytrium strain glycerol tubes, inoculate them into 500mL Erlenmeyer flasks containing 200mL of the above seed medium, and culture them in a shaker at 25°C at 180rpm for 48 hours, then Take 120mL of culture solution and put it into a 5L fermenter with 3L of seed medium according to the inoculation amount of 4%, and cultivate it for 48 hours at a culture temperature of 25°C, a stirring speed of 180rpm, and an aeration ratio of 1.2vvm;
[0041] (4) Put 2.4L of the secondary seed solut...
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