Schizochytrium limacinum fermentation medium

A fermentation medium, technology of Schizochytrium, applied in fermentation, microorganism-based methods, microorganisms, etc., can solve the problems of shortening the service life of equipment, seriousness, corrosion, etc., to reduce investment in fixed assets, promote synthesis, and promote growth Effect

Active Publication Date: 2014-04-23
HUBEI XINHE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The patent of authorized announcement number CN 101519676B discloses the components of the fermentation medium, the amount of sea crystal is 0.8-1.5g / 100ml; the patent of authorized announcement number CN 1264967C discloses the medium components of Schizochytrium OUC88, which contains 20- 80% seawater; sea crystal or seawater is used as one of the components of the fermentation medium, and the concentration of inorganic salts containing chloride ions is generally 5.0-25.0g / L, which is much higher than the tolerable concentration of general fermentation tank materials (of which T304 Stainless steel, its resistance to chloride ion concentration < 0.2g / L; T316 stainless steel, its resistance to chloride ion concentration < 1.0g / L), the corrosion is serious during the long-term operation of the equipment, which shortens the service life of the equipment, so the fermentation tank and The material quality of the pipeline is high, which increases the investment in fixed assets and depreciation costs in the fermentation process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] (1) Add 50mL seed medium (glucose 50.0g / L, yeast extract 5.0g / L, peptone 3.0g / L, MgSO 4 2.0g / L, KH 2 PO 4 2.0g / L), the inoculum amount was 4%, and cultured for 2 days at a culture temperature of 25°C and a shaker speed of 180rpm;

[0013] (2) Add 50mL of fermentation medium into a 250mL Erlenmeyer flask, and inoculate Schizochytrium into the fermentation medium at an inoculum of 4%;

[0014] Fermentation medium components:

[0015] Ⅰ. Glucose 100.0g / L, yeast extract 15.0g / L, MgSO 4 1.0g / L, KH 2 PO 4 3.0g / L, NaNO 3 4.0g / L, Na 2 SO 4 12.0g / L;

[0016] Ⅱ. Glucose 100.0g / L, yeast extract 15.0g / L, MgSO 4 1.0g / L, KH 2 PO 4 3.0g / L, NaNO 3 12.0g / L, Na 2 SO 4 5.0g / L;

[0017] Ⅲ. Glucose 100.0g / L, yeast extract 15.0g / L, MgSO 4 1.0g / L, KH 2 PO 4 3.0g / L, NaNO 3 9.0g / L, Na 2 SO 4 8.0g / L;

[0018] CK: glucose 100.0g / L, yeast extract 15.0g / L, MgSO 4 1.0g / L, KH 2 PO 4 3.0g / L, NaNO 3 4.0g / L, sea crystal 10.0g / L, other culture conditions are the same as above; ...

Embodiment 2

[0025] (1) Add 50mL seed medium (glucose 50.0g / L, yeast extract 5.0g / L, peptone 3.0g / L, MgSO 4 2.0g / L, KH 2 PO 4 2.0g / L), the inoculum amount was 4%, and cultured for 2 days at a culture temperature of 25°C and a shaker speed of 180rpm;

[0026] (2) Add 50mL of fermentation medium into a 250mL Erlenmeyer flask, and inoculate Schizochytrium into the fermentation medium at an inoculum of 4%;

[0027] Fermentation medium components:

[0028] Ⅰ. Glucose 100.0g / L, yeast extract 15.0g / L, MgSO 4 1.0g / L, KH 2 PO 4 3.0g / L, NaNO 3 7.0g / L, K 2 SO 4 2.0g / L, Na 2 SO 4 8.0g / L, KNO 3 3.0g / L; conductivity 27.22ms;

[0029] Ⅱ. Glucose 100.0g / L, yeast extract 15.0g / L, MgSO 4 1.0g / L, KH 2 PO 4 3.0g / L, NaNO 3 6.0g / L, K 2 SO4 3.0g / L, Na 2 SO 4 5.0g / L, (NH 4 ) 2 SO 4 1.0g / L; conductivity 27.47ms;

[0030] Ⅲ. Glucose 100g / L, yeast extract 15.0g / L, MgSO 4 1.0g / L, KH 2 PO 4 3.0g / L, NaNO 3 4.0g / L, K 2 SO 4 5.0g / L, Na 2 SO 4 12.0g / L, KNO 3 5.0g / L, (NH 4 ) 2 SO 4 3.0g / L...

Embodiment 3

[0038] (1) Preparation of seed medium: glucose 50.0g / L, yeast extract 5.0g / L, peptone 3.0g / L, MgSO 4 2.0g / L, KH 2 PO 4 2.0g / L; the initial pH value is 6.0, sterilized at 121°C for 30min, cooled for later use;

[0039] (2) Preparation of fermentation medium: glucose 100g / L, yeast extract 15.0g / L, MgSO 4 1.0g / L, KH 2 PO 4 3.0g / L, NaNO 3 12.0g / L, Na 2 SO 4 5.0g / L; the initial pH value is 6.0, sterilized at 121°C for 30min, cooled for later use;

[0040] (3) Take 10mL-20°C freezer-stored Schizochytrium strain glycerol tubes, inoculate them into 500mL Erlenmeyer flasks containing 200mL of the above seed medium, and culture them in a shaker at 25°C at 180rpm for 48 hours, then Take 120mL of culture solution and put it into a 5L fermenter with 3L of seed medium according to the inoculation amount of 4%, and cultivate it for 48 hours at a culture temperature of 25°C, a stirring speed of 180rpm, and an aeration ratio of 1.2vvm;

[0041] (4) Put 2.4L of the secondary seed solut...

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PUM

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Abstract

The invention relates to a schizochytrium limacinum fermentation medium. By improvement of a fermentation medium formula, the seawater crystal in the medium is replaced by other inorganic salts without chlorine, thereby not only avoiding chloride ion seriously corroding the fermentation equipment, and but also being in favour of DHA accumulation.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a Schizochytrium fermentation medium. Background technique [0002] Schizochytrium limacimum is mainly obtained from the marine environment. In order to meet its suitable growth conditions and the demand for a large amount of DHA oil synthesis, and realize the large-scale fermentation production of Schizochytrium, cheap sea crystals or seawater are selected as the medium group. point. The patent of authorized announcement number CN 101519676B discloses the components of the fermentation medium, the amount of sea crystal is 0.8-1.5g / 100ml; the patent of authorized announcement number CN 1264967C discloses the medium components of Schizochytrium OUC88, which contains 20- 80% seawater; sea crystal or seawater is used as one of the components of the fermentation medium, and the concentration of inorganic salts containing chloride ions is generally 5.0-25.0g / L, which is much hig...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/645
Inventor 朱圆敏余龙江陈伟王勇宁凡钱凯
Owner HUBEI XINHE BIOTECH
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