Herbicide application of xanthatin extracted from Xanthium italicum
A technology of Italian cocklebur and cockleburin, applied in the direction of herbicide and algicide, application, biocide, etc.
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Embodiment 1
[0031] Example 1: Extracting Xanthin from Xanthium cockleburi as a herbicide
[0032] The purified Xanthin with a purity of 98% was prepared with acetone to a concentration of 1000 μg / ml, and then diluted to 10 μg / ml, 50 μg / ml, 250 μg / ml, and 1000 μg / ml. Place the filter paper in a petri dish with a diameter of 9 cm, and then add 3 ml of xanthine solution with a concentration of 10 μg / ml, 50 μg / ml, 250 μg / ml, and 1000 μg / ml respectively. After the acetone is completely volatilized, add 3ml of distilled water to each petri dish, and add 10 seeds of poison lettuce, bluegrass, camel grass and retroflexus amaranthus to each petri dish, and each treatment is repeated three times; After sealing the film, place it in a 25°C incubator and culture it in the dark for 5 days, then measure the seedling height and root length of the seedlings.
[0033] Statistical method of data: Firstly, one-way variance was used to detect whether the difference between the data of each group was signifi...
Embodiment 2
[0035] Example 2: Extracting Xanthin from Xanthium fruticosa as a herbicide and comparison of the action intensity of the commercial herbicide promethazine
[0036] METHODS: Using the above method, the commercial herbicide promethazin was also formulated into a concentration gradient of 10, 50, 250, and 1000 μg / ml, and the seeds of lettuce, bluegrass, camelus and retroflexus were cultivated at 25°C, and the seeds were grown at 25°C. After 5 days, root length and seedling height were measured for statistical analysis.
[0037] Results: Under the action of low concentration of 10μg / ml, except bluegrass, the growth of other tested plant seedlings were significantly affected, and the effect of concentration gradient appeared. When the concentration reached 50 μg / ml, the root lengths of lettuce, bluegrass, camel and amaranthus were 46%, 72%, 13% and 27% of the control; The root lengths of , bluegrass, camel and amaranthus were 41%, 66%, 6% and 17% of the control in turn. When the...
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