Preparation method of high-purity 11S glycinin and application thereof

A technology of soybean globin and protein, which is applied in the direction of chemical instruments and methods, plant peptides, peptide sources, etc., can solve the problems of complicated and lengthy separation process, limit the application of two kinds of proteins, and low protein purity, and achieve simple process and easy assembly line Separation and purification, the effect of improving purity
CN103755792BInactive Publication Date: 2014-12-31北京龙科方舟生物工程技术有限公司
2 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
北京龙科方舟生物工程技术有限公司
Publication Date
2014-12-31
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a preparation method of high-purity 11S glycinin and an application thereof. The preparation method of high-purity 11S glycinin, disclosed by the invention, comprises the following steps: (1), obtaining a protein crude extraction liquid from defatted soybean powder through an acid precipitation method; (2), performing strong anion exchange chromatography on the crude extraction solution in the step (1) for separation and purification, and collecting a target eluant 1; and (3), performing ceramic hydroxyapatite chromatography on the target eluant 1 for separation and purification, and collecting a target eluant 2, namely, a target protein, wherein the target protein is glycinin with a sedimentation coefficient 11S. The preparation method disclosed by the invention is simple in process, easy to separate and purify in a production line, and convenient to produce industrially.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to a preparation method and application of high-purity 11S soybean globinin. Background technique

[0002] Soybean is not only an excellent oil crop, but also an ideal edible protein resource. With the development of modern technology and the deepening of people's understanding of the nutritional and functional properties of soybean protein, defatted soybean flour with high protein content and its separated and purified protein have become Widely used in animal breeding, food processing and other fields.

[0003] According to the sedimentation coefficient of ultracentrifugation, soybean protein can be divided into 2S, 7S, 11S and 15S glycinin. 7S and 11S globulins account for more than 80% of the total soybean protein content, and there are significant differences in their functional properties (water solubility, emulsification, surface activity, etc.) and nutritional properties. Egg whites are highly emulsifying.

[0004] The ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More