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Cutting operation liquid for equine animal embryo, and preparation method and application thereof

A technique for manipulating fluid and embryos, applied in the field of animal embryo transfer

Inactive Publication Date: 2014-04-30
QINGDAO DERUI JUNFA BIOLOGY TECH +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems of traditional embryo segmentation operation solution, such as difficult source of drugs, high price, cumbersome preparation steps and the need to use and prepare immediately, the present invention provides a Large-scale production of embryo cutting operation liquid for non-surgical embryo transfer of equine animals and its preparation method and application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] An equine animal embryo segmentation operation liquid, including 5000ml of sodium lactate mixture as the main liquid, each 1000ml of sodium lactate mixture contains 500,000 units of penicillin, 500,000 units of streptomycin and calf serum with a volume percentage of 10%.

[0023] Weigh 27g of sodium lactate, 16g of sodium chloride, 23g of potassium chloride, 5.5g of calcium chloride and 1.6g of magnesium chloride, add 5000ml of distilled water to mix, place in a thermostat at 37°C for 6 hours, and set aside; weigh 0.4g of astragalus polysaccharide Put it into a sterile beaker, add it to the spare mixed solution into the sterile beaker, stir to fully dissolve the astragalus polysaccharide, and obtain a sodium lactate mixed solution; before the embryo segmentation operation, add penicillin, Streptomycin and calf serum can be used to obtain the embryo segmentation operation solution.

[0024] Use the embryo segmentation operating solution of this embodiment to perform the ...

Embodiment 2

[0026] An equine animal embryo segmentation operation solution, including 5000ml of sodium lactate mixed solution as the main solution, every 1000ml of sodium lactate Ringer injection contains 1 million units of penicillin, 1.2 million units of streptomycin and 3% inactivated horse serum by volume.

[0027] Weigh 10.5g of sodium lactate, 6g of sodium chloride, 13.5g of potassium chloride and 9g of calcium chloride, add 5000ml of distilled water to mix, place in a thermostat at 37°C for 3 hours, and set aside; weigh 1.4g of astragalus polysaccharide into Add it to the sterile beaker, add it to the spare mixed solution, stir to fully dissolve the astragalus polysaccharide, and get the sodium lactate mixed solution; before the embryo segmentation operation, add penicillin and streptomycin to the sodium lactate mixed solution according to the component content And inactivated horse serum, the embryo segmentation operation solution can be obtained.

[0028] The operating liquid for...

Embodiment 3

[0030] An equine animal embryo segmentation operation solution, including 4000ml of sodium lactate mixture as the main solution, wherein every 1000ml of sodium lactate mixture contains 800,000 units of penicillin, 800,000 units of streptomycin and 5% inactivated horse serum by volume; Wherein, the content of each component in the sodium lactate mixed solution is: every 1000ml sodium lactate mixed solution contains 26g of sodium lactate, 10.8g of sodium chloride, 21g of potassium chloride and 16.8g of calcium chloride.

[0031] Weigh 26g of sodium lactate, 10.8g of sodium chloride, 21g of potassium chloride, and 16.8g of calcium chloride, add 4000ml of distilled water to mix, place in a thermostat at 37°C for 1 hour, and obtain a sodium lactate mixture for use; embryo division operation Before, add penicillin, streptomycin and inactivated horse serum to the sodium lactate mixture according to the component content to obtain the embryo segmentation operation solution.

[0032] T...

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PUM

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Abstract

The invention discloses a cutting operation liquid for equine animal embryos, and a preparation method and application thereof. The cutting operation liquid includes a sodium lactate mixed solution, penicillin, streptomycin, and one, two or three selected from bovine serum albumin, calf serum and inactivated equine serum. The preparation method includes the steps of: placing the sodium lactate mixed solution in a thermostat at 37 DEG C for 0.5-6 h for standby; and adding penicillin, streptomycin and one, two or three selected from bovine serum albumin, calf serum and inactivated equine serum into the sodium lactate mixed solution in accordance with the component contents, so as to obtain the cutting operation liquid for embryos. The cutting operation liquid is suitable for non-surgical method for embryo transplantation of horses, cattle and donkeys. The invention has the beneficial effect of convenience for material acquisition, low price, simple preparation and convenience for usage, is very suitable for scale production of non-surgical method for embryo transplantation of equine animals, and is favorable for popularization and application of embryo transplantation technology.

Description

technical field [0001] The invention belongs to the field of animal embryo transplantation, and in particular relates to an equine animal embryo cutting operation liquid and its preparation method and application. Background technique [0002] Embryo bisection is a new biological technology that cuts early embryos into two or four equal parts with the help of micromanipulation technology or manual operation, and then transplants them to recipient dams, so as to obtain identical twins or multiple births. Offspring from the same embryo have the same genetic material, so embryo division can be seen as one of the methods of asexual reproduction or cloning of animals. [0003] Embryo segmentation operation liquid is a nutritional protection liquid used for embryo segmentation operation after obtaining embryos. Most of the operating fluids for embryo segmentation are tissue culture fluids. At present, the commonly used embryo cutting fluids in China mainly include Duchenne's phos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/073
Inventor 张志鹏张肖军马玉萍
Owner QINGDAO DERUI JUNFA BIOLOGY TECH