Cylindrical air-lift type high-efficient photobioreactor for microalgae culture and application thereof

A technology for photobioreactor and microalgae cultivation, which is applied in the field of cylindrical air-lift high-efficiency photobioreactors, and can solve the needs of commercial reactors with complex operation, inability to adjust light intensity and quality, and neglect of light, etc. problems, to achieve the effect of improving photosynthesis efficiency, simple installation and operation, and preventing biological pollution

Active Publication Date: 2015-02-18
INST OF AQUATIC LIFE ACAD SINICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there may still be some deficiencies in the closed reactors developed and applied at present. For example, some commercial reactors have complicated operation and high investment costs, and some commercial reactors and self-developed reactors may ignore the cultivation of microalgae. The demand for light in the process, such as the light transmission supply problem when the algae cell density increases, cannot adjust the appropriate light intensity and light quality for specific microalgae species, etc.

Method used

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  • Cylindrical air-lift type high-efficient photobioreactor for microalgae culture and application thereof
  • Cylindrical air-lift type high-efficient photobioreactor for microalgae culture and application thereof
  • Cylindrical air-lift type high-efficient photobioreactor for microalgae culture and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] A cylindrical air-lift photobioreactor for microalgae cultivation, comprising a light-transmitting culture tank 1, and characterized in that it further comprises a lighting control device 2 arranged outside the light-transmitting culture tank 1, and arranged in the light-transmitting culture tank 1. The temperature control device 3 inside the tank 1 and the gas supply device 4 arranged at the bottom of the light-transmitting culture tank 1, the top of the light-transmitting culture tank 1 is provided with an inoculation sample adding port 6 and an air outlet 7, and the air outlet is provided with a second filter. 26, the bottom of the transparent tank 1 is provided with an algae liquid collection port 11, and the algae liquid collection port 11 is provided with a valve 27, the temperature control device 3 includes a temperature probe 8, a quartz glass temperature control rod 12, a temperature controller 13 and Jacket 9, the jacket 9 extends from the top of the light-tran...

Embodiment 2

[0061] Utilize the cylindrical airlift photobioreactor described in Example 1 to be applied to the photosynthetic high-density autotrophic culture of microalgae, taking the model cyanobacteria Synechocystis sp.PCC6803 as an example, the details are as follows:

[0062] 1. Sterilization of culture medium and light-transmitting culture tank. The autotrophic medium adopts conventional BG11 medium. The BG11 medium is added to the light-transmitting culture tank. The height of the medium is 80% of the height of the tank. It is sterilized by high pressure steam together with the light-transmitting culture tank. During sterilization, the temperature probe is changed from out of the jacket.

[0063] 2. Installation of cylindrical airlift photobioreactor. Install light-emitting diode groups on the inner wall of the housing of the lighting control device, the light quality is white light, and the light intensities of the 6 groups of light-emitting diode groups are 15, 15, 15, 15, 30 an...

Embodiment 3

[0070] The cylindrical airlift photobioreactor described in Example 1 was applied to the photosynthetic high-density autotrophic culture of microalgae, and Chlorella vulgaris (from the Freshwater Algae Species Library of the Chinese Academy of Sciences, No. FACHB-1068) was used as the For example, as follows:

[0071] 1. Sterilization of culture medium and light-transmitting culture tank. The autotrophic medium adopts the conventional BG11 medium. The BG11 medium is added to the light-transmitting culture tank. The height of the medium is 70% of the height of the tank. The high-pressure steam sterilization together with the light-transmitting culture tank is carried out. out of the jacket.

[0072] 2. Installation of cylindrical airlift photobioreactor. Light-emitting diode groups are installed on the inner wall of the housing of the lighting control device, the light quality is red light, and the light intensity of the 6 groups of light-emitting diode groups is 40μmol photo...

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Abstract

The invention discloses a cylindrical air-lift type high-efficient photobioreactor for microalgae culture and an application thereof. The cylindrical air-lift type high-efficient photobioreactor comprises a light transmission culture tank, an illumination control device, a temperature control device and a gas supply device, wherein the illumination control device is arranged outside the light transmission culture tank, the temperature control device is arranged inside the light transmission culture tank, the gas supply device is arranged at the bottom of the light transmission culture tank, an inoculation sample injection hole and an air outlet are formed in the top of the light transmission culture tank, a second filter is arranged at the air outlet, an algae liquid collecting hole is formed in the bottom of a light transmission culture tank body, and a valve is arranged at the algae liquid collecting hole. The cylindrical air-lift type high-efficient photobioreactor has the advantages that the cylindrical tank body with a large proportion of the height to the diameter is adopted, the illumination control device with replaceable adjustable light intensity and light quality is arranged, the conditions of the self shielding phenomenon of algae cells generated after the increase of microalgae culture density and the requirements of specific algae varieties on different light quality are solved, the sufficient luminous energy supply is ensured, the photosynthetic efficiency of microalgae is improved, and the high-density culture is easy to realize.

Description

technical field [0001] The invention relates to the field of biotechnology, and more particularly to a cylindrical airlift high-efficiency photobioreactor for microalgae cultivation and application thereof, which is applied to targeted microalgae photosynthetic high-density autotrophic cultivation. Background technique [0002] Microalgae are prokaryotic or eukaryotic unicellular photosynthetic microorganisms that are very efficient solar energy converters, distributed in fresh or salt water, by absorbing light energy, water and CO transmitted from the water environment 2 By accumulating biomass, light energy can be converted into chemical energy and stored in cells in the form of organic matter such as oil or starch. As the oldest lower photosynthetic organisms, microalgae can directly utilize sunlight, CO 2 And simple nutrients such as N and P grow rapidly and synthesize a variety of high-value active substances such as lipids (mainly triglycerides), proteins, polysacchar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12M1/38C12M1/36C12M1/04C12N1/12C12R1/89
CPCC12M21/02C12M23/08C12M29/04C12M31/10C12M41/06C12M41/12C12N1/12
Inventor 王强陈辉何晨柳
Owner INST OF AQUATIC LIFE ACAD SINICA
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