Superficial-fungi separated culture identification system
A technology for isolating and culturing fungi, applied in the field of identification systems, can solve the problems of inability to rule out fungal infection, inability to determine pathogenic bacteria, etc.
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[0027] The present invention will be further described in the following specific embodiments:
[0028] 1. Medium preparation: DIM medium (modified dermatophyte medium) Ingredients: soy peptone 10g; glucose 20g; agar 20g; 1.6% bromocresol purple solution 1ml; chloramphenicol 0.1g; gentamicin 0.1g ; Add purified water to 1000ml; adjust the pH value to 6.1±0.1 with 1M HCl.
[0029] Preparation method: add soy peptone, glucose and agar to water, heat to dissolve under slow heat; while stirring, add bromocresol purple solution, adjust pH with 1M HCl, autoclave at 121°C for 15min. Cool to 50~55℃, add sterilized filtered chloramphenicol and gentamicin, and pack them into one compartment of a three-compartment plate, such as figure 1 As shown, and cool.
[0030] Urea culture medium components: tryptone 1g; sodium chloride 5g; glucose 50g; potassium dihydrogen phosphate 2g; 0.4% phenol red solution 3mL; agar 20g; 20% urea solution 100mL; chloramphenicol 0.1g; gentamicin 0.1 g; add purified...
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