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Superficial-fungi separated culture identification system

A technology for isolating and culturing fungi, applied in the field of identification systems, can solve the problems of inability to rule out fungal infection, inability to determine pathogenic bacteria, etc.

Inactive Publication Date: 2014-05-07
ZHUHAI ENCODE MEDICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A positive microscopic examination can be confirmed as a fungal infection, but generally the causative bacteria cannot be identified; a negative microscopic examination cannot rule out a fungal infection. If fungal infection is highly suspected, direct microscopic examination or fungal culture can be repeated multiple times

Method used

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  • Superficial-fungi separated culture identification system

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Embodiment Construction

[0027] The present invention will be further described in the following specific embodiments:

[0028] 1. Medium preparation: DIM medium (modified dermatophyte medium) Ingredients: soy peptone 10g; glucose 20g; agar 20g; 1.6% bromocresol purple solution 1ml; chloramphenicol 0.1g; gentamicin 0.1g ; Add purified water to 1000ml; adjust the pH value to 6.1±0.1 with 1M HCl.

[0029] Preparation method: add soy peptone, glucose and agar to water, heat to dissolve under slow heat; while stirring, add bromocresol purple solution, adjust pH with 1M HCl, autoclave at 121°C for 15min. Cool to 50~55℃, add sterilized filtered chloramphenicol and gentamicin, and pack them into one compartment of a three-compartment plate, such as figure 1 As shown, and cool.

[0030] Urea culture medium components: tryptone 1g; sodium chloride 5g; glucose 50g; potassium dihydrogen phosphate 2g; 0.4% phenol red solution 3mL; agar 20g; 20% urea solution 100mL; chloramphenicol 0.1g; gentamicin 0.1 g; add purified...

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Abstract

The invention discloses a superficial-fungi separated culture identification system capable of identifying multiple different strains. The superficial-fungi separated culture identification system comprises a three-separated plate, wherein each grid on the three-separated plate respectively contains a DIM medium, a urea medium and a BCP-MS-G medium; the DIM medium is light green; the urea medium is yellow; the BCP-MS-G medium is grey blue; the three-separated plate is sealed and packaged in a sterile manner, and is stored in a refrigerator with the temperature being 2-8 DEG C. The superficial-fungi separated culture identification system has the advantages that fungi of the pathogenic fungi can be rapidly identified according to the morphology, the character and the color of the formed colony by combination of the characteristics of size, morphology and the arrangement rule of hypha or spores under a microscope. The superficial-fungi separated culture identification system can be applied in the field of strain identification.

Description

Technical field [0001] The invention relates to an identification system, in particular to a shallow fungal separation culture identification system. Background technique [0002] The superficial fungi are mainly Dermatophytes, which invade the skin, hair, nails and other keratinized tissues and cause ringworm. They are also called Ringworms. They are divided into three genera and 37 species. Septal hyphae and articular spores can be seen in the focus of ringworm disease. The hyphae penetrate deep into the keratinized tissue to produce vegetative mycelium, which is intertwined vertically and horizontally into a network. The spores can be arranged in chains or scattered. Spores can be seen on the hair shaft. They are arranged in thick sheaths outside (exo-hair infection) or clusters inside the hair shaft (intra-hair infection). After incubating in Sabol's medium for 1 to 3 weeks, filamentous colonies can be formed, producing various spores and hyphae. According to the morphology...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34C12Q1/04
CPCC12Q1/045
Inventor 孙宜峰龚惠勇曾冰冰
Owner ZHUHAI ENCODE MEDICAL ENG
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