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Function and application of IRF7 gene in stent and carotid endarterectomy restenosis

A technology for restenosis and vascular stenosis, which is applied to the function and application field of IRF7 gene in restenosis after stent and endarterectomy, which can solve the problems of high incidence and affect the treatment effect, and achieve the effect of inhibiting restenosis

Inactive Publication Date: 2014-05-14
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Currently, there is no cure for this kind of disease. The main treatment of vascular surgery is vascular reconstruction of occluded segment, including balloon dilation, stent placement, and arterial bypass surgery, etc. However, the incidence of restenosis after vascular reconstruction is high (30 % to 60%), which greatly affected the therapeutic effect, so far restenosis after vascular reconstruction is still a clinical problem

Method used

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  • Function and application of IRF7 gene in stent and carotid endarterectomy restenosis
  • Function and application of IRF7 gene in stent and carotid endarterectomy restenosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Mouse Vascular Injury Model (VI) Obtained

[0029] 1. Experimental animal grouping: WT and IRF7-KO mice aged 8-10 weeks and weighing 24-27g were used and divided into four groups: WT vascular injury group; WT sham operation group; IRF7-KO vascular injury group; IRF7 -KO sham operation group, 60 mice in each group. 20 mice in each group were sacrificed 14 days and 28 days after the operation, and blood vessels in the injured segment were collected for analysis.

[0030] 2. Operation procedure of mouse vascular injury model:

[0031] 1) Accurately weigh the body weight (g) of the mouse in dynamic mode with an electronic balance, accurately prepare 3% pentobarbital sodium solution with double distilled water, shake gently to dissolve it fully, and use 80mg / kg body weight dose to calculate Accurately extract the corresponding volume of pentobarbital sodium solution with a 1mL syringe, and intraperitoneally inject the anesthetized mouse. After the mouse is fully ...

Embodiment 2

[0036] Example 2 Determination of Intimal Neogenesis in Vascular Injury Model (VI) Mice

[0037] 1. Mice Harvesting

[0038] 1) Anesthetize the mouse, cut the heart and let the blood out.

[0039] 2) Cut the carotid artery from the proximal bifurcation of the carotid artery, take 0.5-0.6cm long, and keep the external carotid artery knot.

[0040] 3) Put the carotid artery in PBS, and gently drain the residual blood in the lumen with micro forceps.

[0041] 4) Put the blood vessel into a 1.5mL EP tube filled with 1mL 4% paraformaldehyde for fixation.

[0042] 2. Pathological detection

[0043] 2.1 Preparation of paraffin specimen slices

[0044] Paraffin specimen sections are prepared by professional pathological staff in the laboratory. The main operating procedures include trimming the heart → processing the embedding frame → washing with running water → dehydrating → transparent → dipping in wax → embedding → sectioning (3 μm) → spreading → drying or baking Backup.

[...

Embodiment 3

[0052] Example 3 Detection of proliferation level of blood vessel wall cells

[0053] The expression of proliferating cell nuclear antigen (PCNA) was detected by immunofluorescence staining. Required primary antibody information: PCNA (#2586; 1:100; mouse; Cell Signaling Technology); Required secondary antibody information: Alexa Fluor 568-conjugated goat anti-mouse IgG (A11004; Invitrogen, Carlsbad, 150 d, CA) .

[0054] The main steps are:

[0055] 1) Baked slices: put the paraffin slices in the oven for more than 30 minutes.

[0056] 2) Dewaxing: Xylene 5min×3.

[0057] 3) Hydration: 100% ethanol 5min×2; 95% ethanol 5min; 70% ethanol 5min; ddH 2 O dipping for 5min×2.

[0058] 4) Citrate tissue antigen repair (high pressure repair): Take a certain amount of pH6.0 citrate antigen repair working solution in the repair box, the amount of repair working solution must be enough to immerse the entire slice, put the repair box into Add an appropriate amount of tap water to th...

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Abstract

The invention discloses function and application of an IRF7 gene in stent and carotid endarterectomy restenosis, belonging to the field of the function and the application of a gene. According to the invention, IRF7 gene knockout mice and wild C57 mice are taken as experimental subjects, a research on vascular injury model mouse intima neogenesis measurement and detection of vascular wall cell proliferation level is carried out through a vascular injury model, and the results show that the IRF7 gene knockout mice realizes more obvious intima neogenesis and cell proliferation in comparison with the wild C57 mice. The invention discloses the function of the IRF7 gene in the stent and carotid endarterectomy restenosis, which mainly means that the IRF7 gene has an effect of inhibiting the restenosis caused by vascular injury, especially the effect that the IRF7 gene can be used for inhibiting the stent and carotid endarterectomy restenosis. According to the abovementioned function, the IRF7 gene can be used for preparing a drug for treating a vascular stenosis disease, especially for preparing the drug for treating the stent and carotid endarterectomy restenosis.

Description

[0001] technical field [0002] The invention belongs to the field of gene function and application, and relates to the function and application of IRF7 gene in restenosis after stent and endarterectomy. Background technique [0003] At present, the number of cardiovascular diseases caused by vascular stenosis is on the rise. At present, there is no cure for such diseases. The treatment methods of vascular surgery include balloon dilation, stent placement and arterial bypass. However, restenosis after vascular reconstruction greatly affected the therapeutic effect. Research on vascular restenosis has been carried out for many years, but so far it is not clear. Studies have shown that in the process of injury formation, the excessive proliferation of neointima and media tissue and the accompanying formation of extracellular matrix are the main pathological basis of restenosis. Under physiological conditions, vascular endothelial cells (EC) can produce a variety of substance...

Claims

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Application Information

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IPC IPC(8): A61K38/21A61P9/10
Inventor 李红良朱丽华张书敏张晓东蒋丁胜向梅
Owner WUHAN UNIV
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