Plant flowering-related protein gmsoc1-like and its coding gene and application
A technology encoding genes and plants, applied to plant flowering-related protein GmSOC1-like and its encoding genes and applications, can solve problems such as slow progress
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Embodiment 1
[0039] Example 1. Acquisition of plant flowering-related protein GmSOC1-like and its encoding gene
[0040] The amino acid sequence of the Arabidopsis AtSOC1 gene was homologously compared in the Genebank database to find the gene with higher homology in soybean, and based on the further obtained full-length cDNA sequence, a PCR primer F1 was designed: 5'-ATGGTGAGAGGAAAGACTCAGCT- 3' and R1: 5'-ATAGCTAGACCTGAGTAGTCCAATGA-3'.
[0041] Take soybean variety Gongxuan No.1 (Glycinemax (L.) Merrill) seedlings cultivated under short-day conditions (8 hours of light and 16 hours of darkness per day) for three weeks, and carefully cut off the tops of their branches with scissors (try to remove young compound leaves ), frozen in liquid nitrogen and stored in a -80°C refrigerator. Use the Trizol method to extract total RNA, reverse transcribe it into cDNA, and then use the cDNA as a template to perform PCR amplification using the above primers F1 and R1, and perform 1% agarose gel electr...
Embodiment 2
[0043] Example 2, the expression pattern of GmSOC1-like gene in different development stages and different tissues of soybean
[0044] The soybean variety Gongxuan No. 1 was planted under short-day conditions (8 hours of light and 16 hours of darkness per day), and the roots, stems, leaves and tops of the plants were harvested after 7, 13, 19, 23 and 27 days after germination. Tissue samples (i.e. shoot top tissues, including later flowers and pods) were frozen in liquid nitrogen and stored at -80°C. The total RNA of each sample was extracted by Trizol method, and then reverse-transcribed into cDNA. The cDNA was used as a template, 5'-AGGCTGTGTGAGCAGTATGGTATC-3' and 5'-TAGATAGACCTGGGTAGTCCAATGA-3' were used as primers, and the GmSOC1-like gene was detected by fluorescent real-time quantitative PCR. (set the expression value at the lowest point in the branch top tissue as 1, and compare the expression levels at other time points with the relative expression level at the lowest ...
Embodiment 3
[0046] Example 3, Diurnal rhythm phenomenon of GmSOC1-like gene expression and its expression pattern under different sunshine length treatments
[0047] 1. Diurnal rhythm of GmSOC1-like gene expression under different sunshine conditions
[0048] The seeds of soybean variety Gongxuan No. 1 were planted in short-day (8h light and 16h dark) and long-day (16h light and 8h dark) conditions, and after 9 days of growth, the top tissue samples of the plants were taken every hour, and the liquid Nitrogen frozen and stored at -80°C. Trizol method extracts the total RNA of each sample respectively, reverse transcription is cDNA again, with this cDNA as template, according to the fluorescent real-time quantitative PCR method of embodiment 2, detects the relative expression of GmSOC1-like gene in each sample, the result is as follows image 3 shown in A and B. The results showed that under short-day conditions, the expression of GmSOC1-like genes in soybeans peaked at midnight (15 hour...
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