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Molecular genetic marker of 300-day-old egg-laying number of Jinghai yellow chicken and application of molecular genetic marker

A molecular genetic marker and egg production technology, applied in the field of poultry breeding, can solve the problems of slow progress and low heritability

Inactive Publication Date: 2014-05-14
YANGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But the reproductive traits are quantitative traits, the heritability is relatively low, and the progress of using traditional breeding methods is slow.

Method used

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  • Molecular genetic marker of 300-day-old egg-laying number of Jinghai yellow chicken and application of molecular genetic marker
  • Molecular genetic marker of 300-day-old egg-laying number of Jinghai yellow chicken and application of molecular genetic marker
  • Molecular genetic marker of 300-day-old egg-laying number of Jinghai yellow chicken and application of molecular genetic marker

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Experimental program
Comparison scheme
Effect test

Embodiment

[0010] 1.1 Experimental animals

[0011] The blood samples of 379 Jinghai yellow chicken hens from the same batch of Jiangsu Jinghai Poultry Industry Group Co., Ltd. were randomly collected. Chicken genomic DNA was extracted by phenol-chloroform extraction method, dissolved in TE, and the concentration and purity were measured by NANODROP1000 nucleic acid concentration analyzer, and stored at -20°C for later use.

[0012] 1.2 Primer design and PCR amplification

[0013] According to the Myf5 gene sequence published in GenBank (accession number: NW_003763474.1), 3 pairs of primers were designed using Primer3.0. The sizes of the amplified products were 173, 247 and 150bp, respectively. The primer sequences are as follows:

[0014] P1: F: 5'-AGATGGAGGTGATGGACAGC-3' (SEQ ID NO: 1)

[0015] R: 5'-GGACGTGTTCCTCTTTCCTCA-3' (SEQ ID NO: 2)

[0016] P2:F: 5'-GCAGCCACTATGAGGGAGAG-3' (SEQ ID NO: 3)

[0017] R: 5'-TTACCATCACATCGGAGCAG-3' (SEQ ID NO: 4)

[0018] P3:F: 5'-CTCTACCGGGAC...

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Abstract

The invention belongs to the field of poultry breeding, relates to an application of a molecular genetic marker of the 300-day-old egg-laying number of Jinghai yellow chicken, and particularly relates to an application of a molecular genetic marker of the 300-day-old egg-laying number of Myf5 gene Jinghai yellow chicken. During screening application, a chicken genome DNA (deoxyribonucleic acid) of a sample to be tested is taken and is amplified by specific primers of which sequences are shown in SEQ ID NO: 1-6 to obtain fragments of 173bp, 247bp and 150bp, the target fragments are subjected to SSCP (Single Strand Conformation Polymorphism) analysis and gel electrophoresis and then developed through silver staining to obtain SSCP graphs of the three DNAs, and individuals, namely individuals combined by H1H3 haplotype, of which genotypes are respectively as shown in figures 1, 7 and 11 in the specification are screened according to strip patterns in the three graphs. The selection effect is remarkable, and the detection method is simple and rapid, is not affected by external environment and can be used for the molecular genetic marker of the 300-day-old egg-laying number of Jinghai yellow chicken.

Description

technical field [0001] The invention relates to the field of poultry breeding, in particular to a method for increasing the number of eggs laid by 300-day-old Jinghai yellow chickens by using haplotype combinations. Background technique [0002] Among the reproductive traits of chickens, egg production is an important economic trait and an important indicator. Increasing egg production can bring huge economic benefits to chicken farms. But the reproductive traits are quantitative traits, the heritability is relatively low, and the progress of using traditional breeding methods is slow. Using the candidate gene method to identify and isolate the QTL related to the genetic variation of these traits is beneficial to the effective selection of poultry economic traits. As the third generation of molecular markers, SNPs are playing an increasingly important role in the research of gene mapping, association analysis, individual disease susceptibility analysis and pharmacogenomics ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 张跟喜王金玉唐莹张涛戴国俊谢恺舟俞亚波
Owner YANGZHOU UNIV
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