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Preparation method and application of yellow green armillaria antioxidant active component

An antioxidant activity, Armillaria yellow-green technology, applied in the application, food preparation, antitoxin and other directions, can solve the problems of inability to guarantee consistency, high reagent consumption, non-repeatability, etc., and achieve a complete and feasible technical solution. , The consumption of reagents is small, and the effect of improving the extraction rate

Active Publication Date: 2014-05-21
贵州贵安精准医学股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has the following defects: (1) The traditional solvent extraction method (liquid-liquid extraction) is used to extract effective active components, and there is no repeatability between batches, that is, it is impossible to guarantee that the material components obtained by the first batch of extraction are consistent with The consistency between the substance components obtained by the second batch of extraction; (2) Using this liquid-liquid extraction method, the distribution process of the substance in different solvents takes a very long time, and multiple extractions (generally three times, new solvent each time), and then combined, which results in a long time period for the entire extraction process and a large amount of reagent consumption

Method used

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  • Preparation method and application of yellow green armillaria antioxidant active component

Examples

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Embodiment 1

[0031] Example 1: Extraction of Antioxidant Active Components of Armillaria Chrysanthemum Fruiting Body

[0032] The preparation method of the antioxidation active component of Armillaria chrysanthemum fruiting body of this embodiment comprises the following steps:

[0033] (1) Armillaria chrysanthemum fruiting bodies were dried in the shade at room temperature, dried and dehydrated at 70°C, and then ultrafinely pulverized at -20°C for 1 min to obtain the superfine powder of Armillaria chrysanthemum fruiting bodies.

[0034] (2) Take 1Kg of the above-mentioned superfine powder of Armillaria chrysanthemum fruiting body, add 6.0L of analytical pure water to extract 3 times at 90°C, each time for 1h, and centrifuge at 8000rpm to collect the supernatant. The supernatant is concentrated to a solid content of 50 mg / mL, and 4 times the volume of absolute ethanol is added for alcohol precipitation treatment, and the supernatant is collected by centrifugation, and the alcohol precipita...

Embodiment 2

[0037] Example 2: Extraction of Antioxidant Active Components of Armillaria Chrysanthemum Fruiting Body

[0038] The preparation method of the antioxidation active component of Armillaria chrysanthemum fruiting body of this embodiment comprises the following steps:

[0039] (1) Armillaria chrysanthemum fruiting bodies were dried in the shade at room temperature, dried and dehydrated at 40°C, and then ultrafinely pulverized at 4°C for 20 minutes to obtain superfine powder of Armillaria chrysanthemum fruiting bodies.

[0040] (2) Take 0.3Kg of the above-mentioned superfine powder of Armillaria chrysanthemum fruiting bodies, add 4.5L of analytically pure water to extract 3 times at 60°C, each time for 2h, and centrifuge at 4000rpm to collect the supernatant. The supernatant was concentrated to a solid content of 200mg / mL, 9 times the volume of absolute ethanol was added for alcohol precipitation treatment and the supernatant was collected by centrifugation, and the alcohol precip...

Embodiment 3

[0043] Example 3: Extraction of Antioxidant Active Components of Armillaria Chrysanthemum Fruiting Body

[0044] (1) Armillaria chrysanthemum fruiting bodies were dried in the shade at room temperature, dried and dehydrated at 50°C, and then ultrafinely pulverized at -10°C for 12 minutes to obtain superfine powder of Armillaria chrysanthemum fruiting bodies.

[0045] (2) Take 0.5Kg of the above-mentioned superfine powder of Armillaria chrysanthemum fruiting body, add 5L of analytically pure water to extract 3 times at 75°C, each time for 1.5h, and centrifuge at 6000rpm to collect the supernatant. The supernatant was concentrated to a solid content of 100 mg / mL, 7 times the volume of absolute ethanol was added for alcohol precipitation treatment and the supernatant was collected by centrifugation, and the alcohol precipitation supernatant was concentrated under reduced pressure (using a rotary evaporator, the process conditions (vacuum degree 0.08Mpa, temperature 60°C, the same...

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Abstract

The invention provides a preparation method of a yellow green armillaria antioxidant active component. The method comprises the steps of carrying out superfine grinding on yellow green armillaria sporocarp to obtain superfine powder; carrying out water extraction treatment on the superfine powder for a plurality of times to obtain liquid supernatant; carrying out alcohol precipitation treatment on the obtained liquid supernatant by absolute ethyl alcohol; concentrating the liquid supernatant treated by alcohol precipitation to obtain a small molecular extract; dissolving the small molecular extract by pure water, and then filtering by an organic filter membrane; carrying solid-phase extraction on the obtained sample solution by C18 or C8 preparative chromatography to obtain the yellow green armillaria antioxidant active component. The method is simple in technology, easy to implement, rapid, efficient, good in repeatability, stable and controllable; the obtained yellow green armillaria antioxidant active component can be applied to preparing antioxidant drugs or health food.

Description

technical field [0001] The invention relates to the technical fields of medicinal chemistry and biomedicine, in particular to a preparation method of an Armillaria chrysanthemum antioxidant active component and the application of the active component in the preparation of antioxidant drugs or health food. Background technique [0002] Armillaria chrysanthemum, commonly known as chanterelle, is a rare edible and medicinal fungus unique to the Qinghai-Tibet Plateau. Wild Armillaria chrysanthemum fruiting bodies are rich in protein, amino acids, vitamins, minerals, alkaloids, volatile oils and polysaccharides, and also contain a small amount of organic acids, flavonoids, cardiac glycosides, volatile oils, steroidal triterpenoids, Glycosides, coumarin, terpenoids, tannins, phenolic compounds, etc., especially the high content of selenium, are the killer of cancer. Studies have shown that Armillaria chrysanthemum has biological activities such as preventing and treating neuritis...

Claims

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Application Information

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IPC IPC(8): A61K36/07A61P39/06A23L1/28A23L1/29A23L31/00
Inventor 张耀洲马琳陶燕铎党军崔今松
Owner 贵州贵安精准医学股份有限公司
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