Skeletal muscle total-organ acellular matrix and preparation method and derivative medical products thereof

Abstract

The invention discloses a skeletal muscle total-organ acellular matrix and derivative medical products thereof as well as a preparation method and a using method of the skeletal muscle total-organ acellular matrix and the derivative medical products thereof. The skeletal muscle total-organ acellular matrix completely preserves a three-dimensional and parallel-arrangement skeletal muscle basement membrane ultramicro structure of the skeletal muscle, a blood vessel matrix network, a muscle-muscle tendon junction, bioactive constituents such as a number of growth factors, hyaluronic acid, glycosaminoglycans, laminin and the like and high-affinity muscle-derived stem cells, and has certain mechanical strength and toughness; thorough decellularization effect is achieved, no obvious immunological rejection is caused, and no ethical and moral issues are caused, therefore, the skeletal muscle total-organ acellular matrix is a skeletal muscle regeneration bracket and platform with the most advantages reported till now. The prepared skeletal muscle total-organ acellular matrix and various derivative medical products thereof can be used for constructing and regenerating skeletal muscles in vitro and in vivo, are suitable for repairing various scoped skeletal muscle defects of all parts, and can be used for hopefully solving the large-scope skeletal muscle defect repairing and muscle generation problem which is still complicated clinically at present.

Description

technical field [0001] The present invention describes the preparation method and application of skeletal muscle whole organ acellular matrix (Whole organ cellular matrix, WOACM) and derived medical products such as microparticles, fluidized compositions, gels and active peptides. Areas involved include tissue engineering and regenerative medicine, surgery, and biomaterials. Background technique [0002] The treatment of skeletal muscle defects, especially the functional reconstruction of large-scale muscle defects, is still a clinical problem so far. Skeletal muscles make up 40-50% of the body weight. Skeletal musculoskeletal trauma is also one of the most common traumas in the human body. According to statistics, 30-55% of sports injuries and 50-70% of war injuries involve skeletal muscles. For minor skeletal muscle injuries, such as various contusions or strains, skeletal muscle tissue can rely on myoblasts (satellite cells) to regenerate muscle fibers to achieve self-r...

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Problems solved by technology

However, it is worth noting that this method still has some limitations, including (1) complicated operation. The ECM materials used in the above research are UBM and SIS. Although the source is convenient, because the average thickness of UBM and SIS is less than 100 μm, it is necessary to To reach the volume of the defective skeletal muscle, hundreds of layers of materials need to be laminated; ②The regenerated skeletal muscle is not a skeletal muscle with full contractile function in the true sense: the substance metabolism of the skeletal muscle is very strong, and the blood flow in the skeletal muscle can reach It is 20-80 times that of the resting state, so the normal human skeletal muscle has relatively large-diameter innervating blood vessels, and there are 1350-3000 capillaries in each square millimeter of muscle; while the blood supply of regenerated skeletal muscle in UBM and SIS implantation areas is the same. Provided by capillaries invading from surrounding tissues, there are no obvious innervating blood vessels, and the density of capillaries is insufficient to provide the blood flow required for severe contraction; ③UBM and SIS fail to reflect the complex structure of the natural skeletal muscle matrix and regenerate the muscle fibers of skeletal muscle Unsatisfactory arrangement

In short, ECM-induced functional regeneration of skeletal muscle is a very promising technology, but there are still places worthy of further study for improvement.

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