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Method for performing colorimetric detection on ractopamine by virtue of AuNPs protected by citric acid radical

A technology of ractopamine and citrate, applied in the field of analytical chemistry, can solve the problems of high cost, long measurement time, unsuitable for rapid detection, etc., and achieve the effect of convenient supervision and rapid detection

Inactive Publication Date: 2014-05-21
JILIN UNIV
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Problems solved by technology

Although these methods have high sensitivity, these methods require expensive equipment, long measurement time, high cost, professional and technical personnel are required to operate, and are not suitable for on-site rapid detection

Method used

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  • Method for performing colorimetric detection on ractopamine by virtue of AuNPs protected by citric acid radical
  • Method for performing colorimetric detection on ractopamine by virtue of AuNPs protected by citric acid radical
  • Method for performing colorimetric detection on ractopamine by virtue of AuNPs protected by citric acid radical

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Embodiment Construction

[0023] Preparation of citrate-protected AuNPs

[0024] Materials / Reagents: HAuCl 4 , Sodium citrate was purchased from Beijing Chemical Reagent Company

[0025] Method: First, 100mL of 1mM HAuCl 4 HAuCl 4 Add the solution into a three-necked flask, heat to boiling, then quickly add 10mL of 38.8mM sodium citrate solution, continue heating until the solution changes from light yellow to deep red, then remove the heating device and continue to stir for 15 minutes after heating for 10 minutes, the obtained After the solution was cooled to room temperature, the precipitate was removed with a 0.4 μm microporous membrane, and the filtrate was placed in a refrigerator at 4°C for detection.

[0026] Results: The ultraviolet absorption spectrum of AuNPs has a maximum absorption peak at 522nm, which is consistent with the literature reports.

[0027] Since the experimental system is affected by the reaction time, it is necessary to study the influence of the time change of the system...

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Abstract

The invention relates to a method for performing colorimetric detection on ractopamine by virtue of AuNPs protected by citric acid radical, which belongs to the technical field of analytic chemistry. The method is characterized in that the AuNPs protected by the citric acid radical can be drawn to be accumulated by ractopamine, so that the color of an AuNPs solution is changed from wine red to blue, the redshift occurs on an absorption peak, and the residue of ractopamine in food can be detected through eyes and by an ultraviolet spectrophotometer. The detection method comprises the steps of preparing AuNPs protected by citric acid radical; and detecting the ractopamine by a colorimetric method. By the method, the ractopamine can be simply, rapidly and sensitively detected, and convenience can be brought to research, production, supervision and the like in future.

Description

technical field [0001] The invention discloses a method for colorimetrically detecting ractopamine by using citrate-protected AuNPs, belonging to the technical field of analytical chemistry. Background technique [0002] Clenbuterol is a general term for a class of drugs, mainly adrenals, β-agonists, and β-agonists. Any substance that can promote the growth of lean meat and inhibit the growth of fat can be called "clenbuterol". . [0003] In China, the so-called clenbuterol mainly includes clenbuterol hydrochloride and ractopamine, etc., and when they are used in livestock feeding at an amount 5-10 times higher than the therapeutic dose, they will have a significant nutritional "redistribution effect" "——Promote protein deposition in animal body, promote fat decomposition and inhibit fat deposition, can significantly increase carcass lean meat rate, weight gain and feed conversion rate, so it has been used as a booster for cattle, sheep, poultry, pigs and other livestock an...

Claims

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Application Information

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IPC IPC(8): G01N21/78
Inventor 孙春燕罗叶丽郭佳佳李莹曹先一许景月沈斐
Owner JILIN UNIV
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