Orange peel dregs seedling raising substrate and preparation method thereof
A technology for citrus peel residue and seedling substrate, applied in the field of seedling substrate, can solve problems such as reducing the cost of seedling substrate raw materials, shortening production cycle, etc., and achieves the effects of improving enzymatic hydrolysis and composting fermentation effect, shortening production cycle, and reasonable water absorption
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Embodiment 1
[0049] The preparation of embodiment 1 liquid microbial bacterial agent
[0050] (1) Preparation of Aspergillus niger CGMCC NO.7927 expansion culture fermentation broth
[0051] The well-preserved Aspergillus niger CGMCC No.7927 slant strains were activated by the strains and the first-, second-, and third-level liquid seeds and seed tanks were gradually expanded to obtain liquid seeds, which were inserted into the fermenter with 6% inoculum. The culture temperature is 30°C, the stirring speed is 400rpm, the ventilation rate (V / V) is 1:2, and the culture time is 12h; then slowly cool down to 12°C at a cooling rate of 2°C / h, and cultivate at a constant temperature for 18h; continue to cool at a rate of 2°C / h Slowly lower the temperature to 3°C. At this time, add the liquid seeds to the fermenter with 4% inoculation amount, and cultivate at a constant temperature for 24 hours; finally, slowly raise the temperature to 12°C at a heating rate of 2°C / h, and cultivate at a constant t...
Embodiment 2
[0065] The preparation of embodiment 2 fermented citrus pomace
[0066] Raw materials: citrus peels are citrus residues after juicing, moisture content 70%, pH value 3.8; livestock manure, moisture content 55%; moisture regulator, crushed wheat straw, rice straw, corn straw, chaff, Mixture of chaff husk and sawdust, crushed particle size 4mm, moisture content 4%;
[0067] The preparation method of described fermented citrus pomace comprises the steps:
[0068] (1) Citrus peel for acid reduction
[0069] Take citrus peels with 55% of the total mass of raw materials, crush them to a particle size of 4mm, add acid-reducing yeast with 0.8% of the mass of citrus peels, and ferment at room temperature for 36 hours, stirring once every 12 hours, until the pH of the citrus peels drops to 6;
[0070] The acid-reduced yeast is composed of the expansion culture fermentation liquid of Pichia pastoris, fission yeast and wine yeast; the concentration of yeast cells in the expansion cultur...
Embodiment 3-6
[0085] The preparation of embodiment 3-6 citrus peel dregs seedling raising substrate
[0086] 1. Embodiment 3-6 raw material composition is as table 1:
[0087] Table 1
[0088] project
fermented citrus zest
Purple soil of Shaximiao Formation
50% Calcium Cyanamide Granules
Egg inactivation rate (%)
Example 3
5 copies
15 copies
0.05 parts
100
Example 4
7 copies
20 copies
0.4 parts
100
Example 5
8 servings
25 copies
0.6 parts
100
Example 6
10 copies
30 copies
0.8 parts
100
[0089] The citrus peel is the fermented citrus peel prepared in Example 2;
[0090] The purple soil of the Shaximiao Formation has a pH value of 6.0 and a water content of 28% after conventional drying. It is crushed and sieved, and the particle diameter is 4mm.
[0091] The 50% calcium cyanamide granules are provided by Ningxia Darong Chemical Metallurgy Co., Ltd., with a particle ...
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