Method for reducing metabolism by-products during producing strain of corynebacterium glutamicum SYPS-062 by L-serine

Abstract

The invention discloses a method for reducing metabolism by-products during producing a strain of corynebacterium glutamicum SYPS-062 by L-serine. The method uses the molecular biological technique to sequentially knock out the sequence of the encoding gene sdaA of L-serinedeaminase as well as the transaminase encoding genes alaT and ilvE in the biosynthetic pathway of L-alanine and L-valine, from the genome of corynebacterium glutamicum SYPS-062 delta S so as to finally construct a recombination strain of corynebacterium glutamicum SYPS-062 delta S-III with knockout sdaA, alaT and ilvE composition.

Description

technical field [0001] The invention relates to a method for reducing metabolic byproducts of L-serine production strain Corynebacterium glutamicum SYPS-062 and application thereof, belonging to the field of industrial biotechnology. Background technique [0002] L-serine, chemical name α-amino-β-hydroxypropionic acid, molecular formula C 3 h 7 NO 3 , molecular weight 105.10. L-serine is a non-essential amino acid, has many important physiological functions, and is widely used in medicine, food, cosmetics and other fields. L-serine is a bottleneck amino acid. In recent years, although there have been many research reports on the production methods of L-serine, there is still a lack of economical and effective industrial production methods so far. my country has not yet realized industrialization, and product demand mainly depends on import. In 2010, my country's serine market demand was 350 tons, of which 20% of domestic production basically adopted the method of hydr...

AI Technical Summary

Problems solved by technology

L-serine is a bottleneck amino acid. In recent years, although there have been many research reports on the production methods of L-serine, there is still a lack of economical and effective industrial production methods so far. my country has not yet realized industrialization, and product demand mainly depends on import

In 2010, my country's serine market demand was 350 tons, of which 20% of domestic production basically adopted the method of hydrolyzing natural protein. This method is time-consuming, has low yield and output, and high cost. pollution problem

The conversion of L-serine to pyruvate, on the one hand, reduces the yield of the target product, and the accumulation of the product pyruvate will affect the physiological characteristics of the bacteria while increasing the content of L-alanine and L-valine in the fermentation broth. The accumulation of amino acids not only causes a large loss of carbon skeleton and nitrogen source, but also increases the complexity of the separation and extraction process of downstream products and affects the purity of intermediate products

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