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Preparation method of polysialic acid-chitosan derivative hydrogel

A technology of chitosan derivatives and polysialic acid, which is applied in the field of preparation of polysialic acid-chitosan derivative hydrogels, can solve the problem of self-polymerization of chitosan and its derivatives, failure to form hydrogels, etc. problem, to achieve the effect of low biodegradability and low immunogenicity

Active Publication Date: 2014-06-04
山东弥美生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since polysialic acid is negatively charged and chitosan is positively charged, the two tend to form precipitates when they are mixed and reacted in aqueous solution, and cannot form hydrogels.
In addition, under the action of some catalysts such as TPP, chitosan and its derivatives under acidic conditions are prone to self-polymerization

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: the preparation of activated polysialic acid

[0022] Mix 500 mg of purified polysialic acid with 50 mL of 0.1 mol / L sodium periodate, and shake in a water bath at 20°C for 15 min in the dark. Then add 100mL ethylene glycol to terminate the reaction, continue to shake for 30min, and the reaction solution is at 0.02% (NH 4 ) 2 CO 3 The solution was dialyzed (molecular weight cut-off 7kDa) for 24 hours, and the activated polysialic acid product was obtained after concentration and freeze-drying.

Embodiment 2

[0023] Embodiment 2: the preparation of polysialic acid-carboxymethyl chitosan hydrogel

[0024] Dissolve carboxymethyl cellulose chitosan with a degree of substitution of 80% and a molecular weight of about 20kDa in a phosphate buffer solution of 20mM and pH 6.5 at a concentration of 5-50 g / L, according to a certain molar ratio of CS:PSA=1 :1 Add activated polysialic acid solution, add NaBH 3 CN (final concentration up to 5 mg / mL), placed in a sealed tube and shaken at 25°C for 4h. After the end, the reaction solution is dialyzed with a dialysis membrane with a molecular weight of 20kDa to remove unreacted small molecules and polysialic acid, and the gel solution is freeze-dried to obtain the final product.

Embodiment 3

[0025] Embodiment 3: the preparation of polysialic acid-hydroxypropyl chitosan hydrogel

[0026] Dissolve hydroxypropyl chitosan with a substitution degree of 55% and a molecular weight of about 20kDa in phosphate buffer solution of 20mM and pH 6.0 at a concentration of 5-50 g / L, according to a certain molar ratio CS:PSA=1:1.5 Add activated polysialic acid solution, add NaBH 3 CN (final concentration up to 5 mg / mL), placed in a sealed tube and shaken at 25°C for 4h. After the end, the reaction solution is dialyzed with a dialysis membrane with a molecular weight of 20kDa to remove unreacted small molecules and polysialic acid, and the gel solution is freeze-dried to obtain the final product.

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Abstract

The invention discloses a preparation method of polysialic acid-chitosan derivative hydrogel and belongs to the technical field of functional biological polymers. The polysialic acid-chitosan derivative hydrogel mainly comprises chitosan derivative and polysialic acid connected to the chitosan derivative and can form hydrogel and nano-particles. The polysialic acid-chitosan derivative hydrogel can be used in fields of drug embedding, medical coating, membrane materials and tissue engineering materials and has antibacterial properties, biocompatibility, low immunogenicity and biodegradability.

Description

technical field [0001] The invention relates to a preparation method of polysialic acid-chitosan derivative hydrogel, which belongs to the technical field of functional biopolymer materials. Background technique [0002] Chitosan (chitosan) is the product of partial or complete deacetylation of chitin (chitin). It is a positively charged cationic polymer that can form water-insoluble composite materials with anionic polymers. It has great potential in the field of biomaterials. great application potential. At pH below 6, chitosan is positively charged, at pH above 6.5, chitosan is deprotonated, chemically active, and at high pH, ​​chitosan forms a network structure (hydrogel or membrane) . [0003] Chitosan is biodegradable and biocompatible with less antigenicity. Many researchers have widely used chitosan in medical and pharmaceutical fields, such as skin dressings and wound repair materials, because chitosan can help wound healing (hemostatic effect, accelerated tissue...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G81/00C08B37/08C08B37/00
Inventor 吴剑荣詹晓北郑志永朱莉
Owner 山东弥美生物科技股份有限公司
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