Blood cell rapid sorting device and manufacturing method thereof

A blood cell and sorting device technology, applied in biochemical cleaning devices, biochemical equipment and methods, enzymology/microbiology devices, etc., can solve the problems of failing to meet clinical needs, slow detection speed, and hindering the application of clinical detection fields and other problems, to achieve the effect of fast detection speed, low cost and simple operation

Inactive Publication Date: 2014-06-04
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These studies are still in the stage of laboratory research. The advantages are low reagent consumption, low cost, high throughput and high efficiency. Although it can be used to screen and detect circulating tumor cells, the detection speed is slow. The milliliter level cannot meet the clinical needs, which hinders its application in the field of clinical testing

Method used

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  • Blood cell rapid sorting device and manufacturing method thereof
  • Blood cell rapid sorting device and manufacturing method thereof
  • Blood cell rapid sorting device and manufacturing method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0041] like figure 1 As shown, it is a schematic structural diagram of a blood cell rapid sorting device in Example 1 of the present invention, figure 2 for figure 1 Partial enlarged view of middle I; image 3 for figure 1 Partial enlarged view of II. figure 2 , image 3 Among them, 2 are circulating tumor cells, 3 are white blood cells, and 4 are red blood cells. This embodiment provides a blood cell rapid sorting device for detecting circulating tumor cells 2 in blood, which is a preferred embodiment of the present invention, the device includes a microfluidic chip 1, and the microfluidic chip 1 includes a supply The pipeline 11 for blood circulation, the centerline of the pipeline 11 is parallel to the fluid direction A; the microfluidic chip 1 is provided with an array of microcolumns 12, and the array of microcolumns 12 is angularly symmetrically arranged with the centerline of the pipeline 11 as the central axis. The microcolumn array 12 in this embodiment has a ...

Embodiment 2

[0045] like Figure 4 Shown is a schematic diagram of a partial structure of a blood cell rapid sorting device according to Embodiment 2 of the present invention. The present embodiment is a preferred embodiment of the present invention, wherein, the cross-sectional shape of microcolumn 12 is triangular, and its circumscribed circle diameter is 40 microns, and the spacing between microcolumns 12 is 20 micrometers, and the arrangement of microcolumn 12 arrays and pipeline The angle formed by the center line of 11 is 3.2 degrees, and the height of the pipe 11 is 40 microns.

[0046] Other structures are the same as in Embodiment 1, and will not be described in detail here.

Embodiment 3

[0048] This embodiment also provides a blood cell rapid sorting device, which is a preferred embodiment of the present invention, wherein the cross-sectional shape of the microcolumns 12 is rectangular, the diameter of the circumscribed circle is 40 microns, and the distance between the microcolumns 12 30 microns, the angle formed by the arrangement of the microcolumn 12 array and the center line of the pipe 11 is 2.5 degrees, and the height of the pipe 11 is 40 microns.

[0049] Other structures are the same as in Embodiment 1, and will not be described in detail here.

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Abstract

Relating to the technical field of blood cell detection, the invention provides a blood cell rapid sorting device, which includes a microfluidic chip. The microfluidic chip comprises a pipeline for blood flow, and the center line of the pipeline is parallel to the fluid direction. Micro-column arrays are arranged in the microfluidic chip, and are in symmetrical arrangement at certain angle with the center line of the pipeline as the central axis. The invention also provides a manufacturing method of the blood cell rapid sorting device. According to the invention, by designing symmetrical micro-column arrays in the microfluidic chip, the micro-column arrays can separate particles of different sizes on the basis of the deterministic lateral displacement principle, and large cells can be screened out, for instance, circulating tumor cells or circulating endothelial cells larger than ordinary blood cells can be enriched and separated. The blood cell rapid sorting device has the advantages of rapid detection speed, simple operation, and low cost.

Description

technical field [0001] The invention relates to the technical field of blood cell detection, in particular to a blood cell rapid sorting device and a manufacturing method thereof. Background technique [0002] Circulating tumor cells are tumor cells that enter the peripheral blood from the primary tumor lesion or metastatic lesion. The detection of circulating tumor cells is of great significance for cancer prevention and treatment. It can be effectively applied to early diagnosis in vitro, rapid evaluation of chemotherapy drugs, and individualized treatment. Traditional tumor diagnosis methods include serum tumor markers, imaging and histopathology. The advantages of serum tumor marker detection methods are that they can provide reference data for imaging diagnosis, low cost and suitable for public screening. The disadvantage is that they are not highly specific. The advantage of imaging examination is to visualize the tumor, but the disadvantage is that it will cause radia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34
CPCC12M23/16
Inventor 刘宗彬陈艳
Owner SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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