Bacillus licheniformis for decomposing feathers of pigeons and application of bacillus licheniformis

A technology of Bacillus licheniformis and feathers, which is applied in the application field of environmental resources in aquaculture, can solve problems such as poor effect, and achieve the effect of promoting ecology

Active Publication Date: 2014-06-04
JINLING INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

We have shown through bacteria isolation and degradation experiments that bacteria that can degrade chicken feathers are not effective in degrading pigeon feathers. Therefore, it is necessary to screen and cultivate a single strain with better decomposition effect

Method used

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  • Bacillus licheniformis for decomposing feathers of pigeons and application of bacillus licheniformis
  • Bacillus licheniformis for decomposing feathers of pigeons and application of bacillus licheniformis
  • Bacillus licheniformis for decomposing feathers of pigeons and application of bacillus licheniformis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Culture identification of bacterial strains.

[0035] 1. Obtaining the original strain

[0036] Sampling 5 grams of pigeon manure deposits from a pigeon farm in Nanjing, directly inoculated the collected soil with a sterilized inoculation loop on an ordinary agar plate, cultured at 37°C for 24 hours, observed the shape of a single colony grown, and the diameter of the bacteria was about 2mm , with irregular edges, pale brown, coarse-shaped colonies ( figure 1 ). Carry out further isolation and culture, after obtaining a single colony, carry out pure culture. Bacterial isolation and culture results: A purely cultured bacterium was obtained through bacterial isolation and culture, named NJ-01. The isolated bacteria were in the shape of petals under a 400-fold low-power microscope ( figure 2 ). The bacteria were observed under the oil microscope after Gram staining, and the result was G with spores. + Medium-sized bacilli, most of which are single, and a ...

Embodiment 2

[0051] Example 2: Fermentation of Bacillus licheniformis CCTCC NO: M2013489.

[0052] (1) Activation of Bacillus licheniformis strain NJ-01

[0053] Streak the Bacillus licheniformis strain NJ-01 on the activation medium with an inoculation loop, and culture it in a constant temperature incubator for 48 hours, and set the culture temperature at 37°C;

[0054] (2) Preparation of seed solution

[0055] After activating the strain described in step (1), inoculate 1 to 2 single colonies into 30 to 40 mL of seed medium with an inoculation loop under aseptic conditions, and shake and culture at 35 to 40° C. for 24 to 36 hours to obtain a seed liquid;

[0056] (3) Preparation of fermentation broth

[0057] Shake flask fermentation: Inoculate the seed solution in a shake flask with a fermentation medium with a volume of 40% to 60% of the volume of the flask at an inoculum volume of 5 to 10%, at 35 to 40°C, at 120 to 200 rpm Shake the bottle at a speed of 48-60 hours to ferment for ...

Embodiment 3

[0063] Embodiment 3: Bacteria observes the decomposition form of pigeon feather flakes.

[0064] The bacteria NJ-01 isolated and cultivated can decompose the pigeon feather flakes without adding any nutrients, and the decomposition results are as follows: Image 6 , Figure 7 , Figure 8 , Figure 9 Control without strain. The feather patterns observed each day are:

[0065] Primitive pinnae: The complete feather stem can be seen, with many slender barbules branching out from it, and several thinner barbules protrude from each barb, and the small hooks on the connected barbules are connected to each other, and the feathers are in the form of a net shape structure.

[0066] On the fifth day, the feather flakes: the quills are still intact, the barbs become soft, the barbs disintegrate, the barb hooks dissolve, the feather network structure disappears, and the appendage part disappears.

[0067] Feather flakes on the 10th day: Feather stems were soft, barbs disappeared, ba...

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Abstract

The invention discloses bacillus licheniformis for decomposing feathers of pigeons. The bacillus licheniformis is classified and named bacillus licheniformis; the strain number is NJ-01; the bacillus licheniformis is preserved in the China Center for Type Culture Collection on Oct.23th, 2013 with the preservation number of CCTCC NO: M2013489. The invention also discloses application of the strain. The new strain disclosed by the invention can be directly used for decomposing the feathers of pigeons, so that waste of the pigeons can be recycled favorably, and ecological and circulating breeding of the pigeons is promoted; the bacillus licheniformis belongs to a safe microorganism and can be used in the fields of decomposition of the feathers of the pigeons, fermentation of meat pigeon breeding waste and biological fertilizers.

Description

technical field [0001] The invention relates to the application field of environmental resources in breeding industry, in particular to a bacterial strain for decomposing pigeon feathers, in particular to a bacillus licheniformis capable of effectively decomposing pigeon feathers and its application. Background technique [0002] With the intensive development of the poultry industry, breeding enterprises, especially large and medium-sized livestock farms, produce a large amount of waste such as feces, feathers, bone meal, and blood residue every day while providing meat, poultry, and eggs. If it is not handled properly, it will often pollute the environment and spread pathogens. Feathers, in particular, are more difficult to recycle than other wastes due to their non-degradable characteristics. According to research reports, the keratin content in feathers is as high as 80%, and is rich in lysine, tryptophan, threonine, methionine, histidine, proline, glycine and some othe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08A23K1/18C12R1/10
Inventor 方光远茅慧华戴鼎震蒋加进张姝
Owner JINLING INST OF TECH
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