The invention discloses a pseudo random sequence generation method for wireless sensor network nodes and an application method thereof, wherein the pseudo random sequence generation method for the wireless sensor network nodes comprises the following steps: step S101: inputting any prime number p and cardinal number R; step S102: setting an initial value of x to be the cardinal number R, initializing i to be 1, and initializing a random sequence S; step S103: setting an intermediate variable t, wherein t is equal to mod(x, p); step S104: assigning S(i), wherein S(i) is equal to floor(x/p); step S105: amending the value of x, wherein x is equal to t*R+S(i), amending the value of i, i is equal to i+1; step S106: judging whether the length of the random sequence S is equal to the length of the random sequence C, if so, carrying out the next step; if not, returning to the step S103: step S107: outputting the random sequence, S is equal to {S(1), S(2),..., S(i)}. New D-sequence generated by the method changes the limited cycle period of original (1/p)RD-sequence so as to enlarge the sequence length; the method is more sensitive for disturbance, and has extremely good autocorrelation, cross correction and probability distribution characteristic.