A kind of high-efficiency extraction process of heparin sodium
An extraction process, the technology of heparin sodium, which is applied in the field of extraction process for extracting heparin sodium, can solve the problems of increased difficulty in preparation and low purity of heparin sodium, and achieve the effects of increased yield, less salt consumption, and less water consumption
Inactive Publication Date: 2016-08-17
GUANGYUAN SHENDA IND
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Problems solved by technology
The extraction rate of heparin sodium is mostly between 100 million IU / 2300-2500 small intestines (the best level is 100 million IU / 1600 small intestines, only a few companies can reach this level), the purity of the extracted heparin sodium is low, often in 70-90IU / mg, which contains a lot of protein, and the color is dark brown, which makes the preparation of clinical medicine more difficult
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Embodiment Construction
[0008] In order to make the purpose of the present invention and the advantages of the technical solution more clear, the present invention will be further described in detail below in conjunction with the accompanying drawings and examples.
[0009] A high-efficiency extraction process for heparin sodium, specifically comprising the following steps:
[0010] (1) Intestinal mucous membrane enzymatic hydrolysis: add water to 1600 small intestine mucous membranes to 10 tons, put them into the reactor, heat up to 55-65°C, keep warm for 10-20min, add 4.8-6.4kg in total according to 3-4g of each small intestine 2709 protease, and add lye to adjust the pH value to 8.5-9.5, and then use the ratio of NaCl to intestinal mucosa as 3:100, that is, add 2.5-3kg of NaCl to the mixture of intestinal mucosa and water per 100kg to adjust the salinity 2-2.5°C, stir for 2-3h, heat up to 75°C, stop stirring for 10-20min and then precipitate naturally, filter and collect the filtrate;
[0011] (2...
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The invention discloses a process for high-efficiently extracting sodium heparin. The process comprises the following steps: the mixture of intestinal mucosa and water undergoes enzymatic hydrolysis, the ratio of intestinal mucosa to water is 1:4, and sodium heparin is obtained by conventional adsorption, increased adsorption, eluting, deposition and purification, and drying. The yield of sodium heparin is improved due to the addition of secondary enzymatic hydrolysis and increased adsorption in the extraction process of the invention, the purity of sodium heparin is improved, particularly, the yield of sodium heparin reaches 0.1 billion IU / 1200-1400 porcine small intestines, is increased by 30-45 percent compared with the conventional process; the purity of sodium heparin produced according to the process reaches 110-130 IU / mg; based on the process for high-efficiently extracting sodium heparin of the invention, the consumption of water is saved by about 40 percent, the consumption of salt is saved by 20-40 percent, and the process is a process for high-efficiently extracting sodium heparin with small water consumption, small salt consumption and high economic benefit.
Description
technical field [0001] The invention relates to an extraction process for extracting heparin sodium from animal casings, in particular to an efficient extraction process for heparin sodium. Background technique [0002] Heparin sodium is a sulfate ester of acidic mucopolysaccharide produced by mast cells of animal connective tissue. Because of its strong anticoagulant effect, it is the drug of choice for preventing and treating thromboembolic diseases such as deep venous thrombosis. With the development of research In-depth, people found that heparin sodium not only has anticoagulant, antithrombotic and blood lipid adjustment effects, but also has various biological functions such as anti-inflammation, anti-allergy, anti-virus, and anti-cancer. Although heparin sodium has been used clinically for more than 60 years, so far there is no product that can completely replace it, so it is still the most important anticoagulant and antithrombotic biochemical drug, and currently it ...
Claims
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IPC IPC(8): C08B37/10
Inventor 常国华郭婷婷
Owner GUANGYUAN SHENDA IND