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A kind of immobilized laccase based on fiber carrier and its preparation method and application

A technology of immobilizing laccase and fiber, which is applied in the direction of fixing on or in the inorganic carrier, fixing on/in the organic carrier, etc., can solve the problem that the recovery rate of enzyme activity is not very high, and achieve strong reuse and operability, the effect of protective activity

Active Publication Date: 2017-01-04
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Usually, this method has higher immobilization efficiency, better protection of laccase activity during immobilization, and less leakage of enzyme during use; but due to the laccase wrapped inside the carrier, it is difficult to participate in In subsequent reactions, the recovery rate of enzyme activity is not very high

Method used

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  • A kind of immobilized laccase based on fiber carrier and its preparation method and application
  • A kind of immobilized laccase based on fiber carrier and its preparation method and application
  • A kind of immobilized laccase based on fiber carrier and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0040] In this example, the most difficult-to-modify polystyrene (PS) electrospun microfiber was used as the carrier for immobilizing laccase, and the average diameter of the fiber was 700 nm.

[0041] First, take 10 mg of fiber and immerse it in 9.8M concentrated sulfuric acid, remove it after 5 minutes, and rinse it repeatedly with deionized water until the pH value of the rinsing liquid does not change before and after rinsing. Thereafter, soak the fibers in an aqueous solution of 1 mg / mL polydiallyldimethylammonium chloride (PDADMAC), remove the fibers after 10 min, wash 3 times with deionized water, and then soak them in 1 mg / mL In the aqueous solution of polyacrylic acid grafted alkyl bromide (PAA-g-Alkyl Bromide), after 10 minutes, the fiber was pulled out and washed 3 times with deionized water. Repeat the operation of soaking and washing the PDADMAC and PAA-g-Alkyl Bromide aqueous solution twice. Thereafter, 10 mg of fiber was dispersed in 16 mL of water, and 2 mL of...

Embodiment 2

[0043] Embodiment 2: immobilized laccase on the surface of polystyrene (PS) microfiber

[0044] Get 10mg fiber (same as Example 1) and immerse in the concentrated sulfuric acid of 9.8M, pull out after 5min, rinse repeatedly with deionized water, until before and after rinsing, the pH value of the rinsing liquid no longer changes. Afterwards, soak the fiber in 1 mg / mL polyethyleneimine (PEI) aqueous solution, remove the fiber after 10 min, wash 3 times with deionized water, and then soak in 1 mg / mL polyacrylic acid (PAA) aqueous solution After 10 min, the fibers were removed and washed three times with deionized water. Repeat the operation of soaking and washing in the PEI and PAA aqueous solution twice, and finally repeat the soaking and rinsing in the PEI aqueous solution again. Since the PEI molecule itself is rich in amine groups, so far, the surface of polystyrene (PS) fiber has introduced molecules rich in amine groups.

[0045] Prepare 3-mercaptopropyltriethoxysilane a...

Embodiment 3

[0046] Embodiment 3: immobilized laccase on the surface of polyethylene terephthalate (PET) microfiber

[0047] In this embodiment, PET yarns are used as the carrier, and the average diameter of the carrier fibers is 50 μm.

[0048] First, take 10 mg of fiber and immerse it in 9.8M concentrated sulfuric acid, remove it after 5 minutes, and rinse it repeatedly with deionized water until the pH value of the rinsing liquid does not change before and after rinsing. Thereafter, soak the fibers in an aqueous solution of 1 mg / mL polydiallyldimethylammonium chloride (PDADMAC), remove the fibers after 10 min, wash 3 times with deionized water, and then soak them in 1 mg / mL Polyacrylic acid grafted alkyl bromide (PAA-g-AlkylBromide) aqueous solution, after 10 minutes, the fiber was pulled out and washed 3 times with deionized water. Repeat the operation of soaking and washing the PDADMAC and PAA-g-Alkyl Bromide aqueous solution twice. Thereafter, 10 mg of fiber was dispersed in 16 mL ...

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Abstract

The invention relates to fiber carrier based immobile laccase and a preparation method and application thereof. The fiber carrier based immobile laccase comprises fiber serving as a carrier, active molecules distributed on the surface of the fiber, a mesoporous silica layer covering the fiber and the active molecules and laccase embedded in the mesoporous silica layer, wherein the mesoporous silica layer is formed by depositing active molecule catalyzed organosilane on the surface of the fiber. The immobile laccase disclosed by the invention is high in enzyme activity recovery rate, high in immobilization efficiency, good in reusability and good in operability. The preparation method is moderate in reaction conditions and greatly protects activity of the laccase. The immobile laccase disclosed by the invention is applicable to such fields as food, textile, papermaking from pulp or sewage treatment.

Description

technical field [0001] The invention relates to the technical field of immobilized enzymes, in particular to a fiber carrier-based immobilized laccase, a preparation method thereof and an application in dye decolorization. Background technique [0002] Enzymes are important catalytic substances in the life activities of organisms, which control the entire metabolism of organisms. In vitro, enzymes are widely used in the fields of pharmaceuticals, food and brewing because of their high efficiency and specificity. However, when the enzyme leaves the relatively stable and mild environment of the organism, it becomes extremely fragile. It is particularly easy to denature and inactivate in the environment of high temperature, strong acidity or alkalinity, and organic solvents, which limits the wide application of enzymes in industry. And because the enzyme is not easy to be separated in the reaction system, the enzyme cannot be used repeatedly, which increases the cost and brin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14C12N11/12C12N11/08C12N11/04C12N11/02
Inventor 蒋兴宇王铈汶孙佳姝陈威
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA