Method for extracting saponin compounds from honeysuckle

A honeysuckle and compound technology, which is applied in the pharmaceutical field to achieve the effects of reducing cost, reducing industrial energy consumption and improving utilization rate

Active Publication Date: 2014-06-18
山东惠普生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the defects of the extraction method of honeysuckle saponins in the prior art, the present invention provides a method for extracting saponins from honeysuckle, the method has high yield of saponins, low energy consumption, simple steps and easy industrial production

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  • Method for extracting saponin compounds from honeysuckle
  • Method for extracting saponin compounds from honeysuckle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for extracting saponins from Flos Lonicerae, comprising the steps of:

[0023] 1) Pulverization: put 1 kg of honeysuckle (Pingyi honeysuckle) into a pulverizer to pulverize, and then spread it into 1 square meter;

[0024] 2) Ultraviolet irradiation: irradiate under ultraviolet light for 10 minutes, and the ultraviolet intensity is 2000uW / cm 2 ;

[0025] 3) Hydrolysis: use deionized water 8 times the weight of honeysuckle as a solvent, maintain at 0.1 atmosphere, hydrolyze at 75°C for 1 hour, and collect the filtrate;

[0026] 4) concentration: the filtrate is placed in a vacuum concentrator, and vacuum concentrated to one-third of the volume of the filtrate;

[0027] 5) Precipitation: add a calculated amount of alcohol so that the alcohol content in the concentrated solution reaches 75% (v / v), and after standing for 1 hour, the chlorogenic acid is completely precipitated, and the filtrate and precipitate are collected by filtration;

[0028] 6) Drying: the ...

Embodiment 2

[0032] A method for extracting saponins from Flos Lonicerae, comprising the steps of:

[0033] 1) Crushing: put 1 kg of honeysuckle into a pulverizer to pulverize, and then flatten it into 1 square meter;

[0034] 2) UV irradiation: UV irradiation for 15 minutes, the UV intensity is 2000uW / cm 2 ;

[0035] 3) Hydrolysis: use deionized water 10 times the weight of honeysuckle as a solvent, maintain a state of 0.1 atmosphere, hydrolyze at 75°C for 1 hour, and collect the filtrate;

[0036] 4) concentration: the filtrate is placed in a vacuum concentrator, and vacuum concentrated to one-third of the volume of the filtrate;

[0037] 5) Precipitation: add a calculated amount of alcohol so that the alcohol content in the concentrated solution reaches 75% (v / v), and after standing for 1 hour, the chlorogenic acid is completely precipitated, and the filtrate and precipitate are collected by filtration;

[0038] 6) Drying: the precipitate was vacuum-dried at 80°C to obtain chlorogeni...

Embodiment 3

[0042] Effects of various factors on the extraction efficiency of saponins and chlorogenic acid

[0043] 1. The influence of ultraviolet irradiation intensity and time, control other conditions unchanged, only change the ultraviolet irradiation parameters, repeat the experiment three times for each group, see Table 1 for specific results

[0044] Table 1

[0045]

[0046] Conclusion: Through analysis, it is found that ultraviolet irradiation can greatly increase the yield of saponins and chlorogenic acid, but with the increase of time, the purity of chlorogenic acid is greatly reduced, which may be due to the decomposition of chlorogenic acid caused by strong light. Little effect on saponins.

[0047] 2. Effect of hydrolysis time on the yield of saponins and chlorogenic acid

[0048] Change the time of hydrolysis, control other conditions unchanged, repeat each group of experiments three times, see Table 2 for specific results

[0049] Table 2

[0050]

[0051] Concl...

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Abstract

The invention relates to a method for extracting saponin compounds from honeysuckle. The method comprises the steps of grinding, ultraviolet radiation, hydrolysis, concentration, precipitation, drying and the like. The method adopts ultraviolet with proper intensity for radiation and deionized water for hydrolysis and avoids using various extraction reagents and decoloring agents. The saponin compounds prepared by the method has high yield and the method has the advantages of low energy consumption, no pollution and simple operating steps and can be widely applied to industrial production.

Description

technical field [0001] The invention belongs to the technical field of pharmacy, and in particular relates to a method for extracting saponins from honeysuckle. Background technique [0002] Honeysuckle is a general term for Chinese medicinal materials and plants. Plant honeysuckle, also known as honeysuckle, is a perennial semi-evergreen winding woody vine of the family Lonicera. The name "Honeysuckle" comes from "Compendium of Materia Medica". Because the honeysuckle flower is white at first, and then turns yellow, it is named honeysuckle. The medicinal material honeysuckle is the dried flower buds or flowers of the same genus Lonicera Lonicera and the same genus. Honeysuckle contains volatile aromatic oils, saponins, chlorogenic acid, flavonoids, etc. It is one of the traditional Chinese medicinal materials, and it is also a raw material for both medicine and food. effect. [0003] Saponin is a kind of glycoside whose aglycone is triterpene or spirosterane compound, m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/355A61P31/04A61P29/00A61P25/20A61P35/00A61K133/00
Inventor 石宏廉吉芳张宗桥庞朝征
Owner 山东惠普生物科技有限公司
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