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Method for taking glucose as substrate to continuously produce polyhydroxylbutyrate valerate (PHBV) in one step by using halophilic mixed bacteria

A technology of mixed halophilic bacteria and glucose, applied in fermentation and other directions, can solve the problems of complex process control, inability to realize continuous production and operation, etc., and achieve the effects of easy engineering operation, increased commercial utilization value, and high yield

Inactive Publication Date: 2014-06-18
BEIJING UNIV OF TECH
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AI Technical Summary

Problems solved by technology

Although the two-step method can increase the yield of PHA, the process cannot achieve continuous production and operation
Due to the complex process requirements in each process, the complexity of process control becomes the main obstacle of this method

Method used

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  • Method for taking glucose as substrate to continuously produce polyhydroxylbutyrate valerate (PHBV) in one step by using halophilic mixed bacteria

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specific Embodiment approach

[0028] Step 1: Collect estuary sediment at the mouth of Tanghe River in Qinhuangdao City, Hebei Province. The sampling site is located 600 meters away from the Bohai Sea. The collected bottom mud is inoculated into the fermenter after sand removal. Inject the substrate aqueous solution to 2L in the fermenter, and the inoculum concentration is 0.102g / L;

[0029] Step 2: Control the temperature of the fermenter at 37±0.1°C, pH=7.2. Open the aeration device and feed air into the fermenter to maintain the dissolved oxygen in the mixed solution in the fermenter at 2 mg / L. Continuous aeration operation for 6 hours, stop aeration;

[0030] Step 3, remove 1.5L of the mixed solution, and the remaining 0.5L is used for the next fermentation;

[0031] Step 4, inject 1.5L substrate into fermenter again,

[0032] Step 5, repeat steps 2-480 times, the mass percentage of PHBV in the reaction solution is not less than 30%;

[0033] Step 6, continue to repeat steps 2-4176 times continuous...

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Abstract

The invention discloses a method for taking glucose as a substrate to continuously produce polyhydroxylbutyrate valerate (PHBV) in one step by using halophilic mixed bacteria, and belongs to the technical field of PHBV production. The method comprises the following steps: collecting bottom mud at an estuary to inoculate into a fermentation tank, and then operating the fermentation tank by adopting an intermittent operation mode; injecting air into the fermentation tank at a constant speed, controlling the dissolved oxygen concentration in a substrate solution at 1-3mg / L, and continuously running for 5-8 hours, wherein the operation conditions of the fermentation tank are as follows: the constant temperature of the fermentation tank is 37+ / -0.1 DEG C, and the pH is 6-8, and discharging 75% mixed solution in the fermentation tank, wherein the mass percent of the PHBV can be up to over 30% by continuous repetition; recovering biomass in the discharged mixed solution, and extracting phytohemagglutinin (PHA). The method is simple to achieve, and engineering operation is easy to carry out, and the produced PHA is a PHBV copolymer, and has high decomposition temperature and low melting temperature. By adopting the method, the production cost of the PHA is reduced by 20-30%.

Description

technical field [0001] The invention relates to a method for producing intracellular polyhydroxybutyrate valeric acid copolyester (PHBV) by using microorganisms, in particular to a method for producing PHBV by using halophilic mixed bacteria as producers to metabolize glucose and biosynthesize in one step. The method can reduce the production cost of PHBV and belongs to the technical field of PHBV production. Background technique [0002] Globally, the accumulation of plastic waste poses a serious threat to the environment due to the extensive use of plastic products by various industries. To combat this problem, the production of biodegradable materials to replace petroleum-based plastics is key to addressing this problem sustainably. Polyhydroxyalkanoate (PHA) is a typical environment-friendly material, which can be 100% biodegradable. PHA can be used as a carbon source and energy storage by many microorganisms, and is considered to be an important raw material that can ...

Claims

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Application Information

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IPC IPC(8): C12P7/62
Inventor 崔有为卢鹏飞彭永臻
Owner BEIJING UNIV OF TECH
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