Preparation method of strong anti-matrix interference type bisphenol A up-conversion fluorescence chromatography test strip

Abstract

The invention discloses a bisphenol-A up-conversion fluorescence chromatography test strip with high matrix interference resistance and a preparation method thereof. The test strip comprises a sample pad, a combination pad, a coating film and a water absorption pad, which are sequentially connected with one another on lining plate, wherein the combination pad is adsorbed with an up-conversion fluorescence-bisphenol-A antibody composite probe mainly comprising an up-conversion fluorescence nano material and a bisphenol-A antibody; a detection mark and a contrast mark are distributed on the coating film; the detection mark comprises a bisphenol-A coating antigen. The preparation method comprises the steps of preparing the combination pad, preparing the coating film and assembling the sample pad, the combination pad, the coating film and the water absorption pad with the lining plate. Excitation wavelengths of the test strip disclosed by the invention are positioned in a near infrared region with low energy, so that the interference of autofluorescence of a biological sample can be effectively avoided, the background fluorescence in detection is reduced, the signal-to-noise ratio is increased, and the bisphenol-A up-conversion fluorescence chromatography test strip is extremely high in sensitivity and specificity; moreover, the preparation method is simple and low in cost; during use, the bisphenol-A up-conversion fluorescence chromatography test strip also has the advantages of simplicity, quickness, accuracy and the like.

Description

technical field [0001] The invention particularly relates to an up-conversion fluorescence chromatography test strip for rapid detection of bisphenol A with strong resistance to matrix interference and a preparation method thereof, belonging to the field of fluorescence immunoassay detection technology and rapid detection of food safety. Background technique [0002] In recent years, it has been found that BPA (BisphenolA, 2,2-bis(4-hydroxyphenyl) propane) is an "Endocrine Disrupting Chemicals (EDCs) that has certain estrogenic properties. , has a certain damage to the male reproductive system, and also has a certain impact on the embryo, can accelerate precocious puberty, or cause mental disorders such as hyperactivity and distraction in children. BPA has DNA oxidative damage at low doses and can induce chromosomal abnormalities, and can affect the host's non-specific immune defense system. Therefore, the hazards of BPA have aroused widespread concern on food and packaging...

AI Technical Summary

Problems solved by technology

Although the existing instrument detection method has the advantages of high sensitivity and wide application range, the sample pretreatment is complicated, the required equipment is expensive, the detection cost is high, and the detection time is long, and only specific professional and technical personnel can operate it. On-site, rapid detection, poor timeliness, difficult to promote, it is difficult to realize rapid and on-site detection of BPA

In addition, although traditional fluorescent test strips and colloidal gold test strips based on organic dyes or fluorescent quantum dots can quickly detect BPA under certain conditions, complex biological samples (blood, urine and saliva) contain complex biological samples, and the excitation light source of dyes and fluorescent quantum dots can simultaneously stimulate the autofluorescence of biological samples in the detection solution and the background fluorescence of the nitrocellulose membrane in the lateral flow test strip, thus affecting the actual detection results of BPA , which reduces the sensitivity and accuracy of detection

The same colloidal gold test strip also has the disadvantages of low sensitivity and narrow detection range.

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