Kit for detecting anthropogenic N terminal-b-type natriuretic peptide precursor

Abstract

The invention discloses a kit for detecting an anthropogenic N terminal-b-type natriuretic peptide precursor. The kit comprises a magnetic particle coupling antibody and an enzyme-labeled antibody, wherein the enzyme-labeled antibody is an antibody labeled by horseradish peroxidase, the antibody is an NT-proBNP monoclonal antibody, and the epitope of the NT-proBNP monoclonal antibody is in amino acid areas contained by 10-31 and 51-76. The kit has the advantages that a new antibody epitope is utilized to establish a new method for detecting the hypersensitization and specificity of a heart function failure marker, namely the N terminal-b-type natriuretic peptide precursor; the detection method can quantitatively detect the N terminal-b-type natriuretic peptide precursor, provides an objective index for heart failure diagnosis, and can also be used for judging the severity of illness; the detection method can be used for predicting prognosis of heart failure, and the objective index can be used as a reference factor of disease risk stratification. The kit has high sensitivity, strong specificity and good accuracy, has good correlation with Elecsys NT-proBNP at the same time, and is suitable for semi-automatic or fully-automatic detection systems.

Description

technical field [0001] The invention relates to the field of immune analysis, in particular to a kit for detecting human N-terminal-B-type natriuretic peptide precursor. Background technique [0002] Heart failure is a group of syndromes caused by various structural or functional diseases of the heart that lead to impaired ventricular filling and / or ejection ability. Due to the decline of ventricular systolic function and impaired ejection function, the cardiac output cannot meet the needs of the body's metabolism, the blood perfusion of organs and tissues is insufficient, and there is congestion in the pulmonary circulation and (or) systemic circulation at the same time. The clinical manifestations are mainly dyspnea and weakness. Physical activity limitation and edema. The incidence of heart failure will continue to increase in the near future, and it is becoming the most important cardiovascular disease in the 21st century. [0003] B-type natriuretic peptide (BNP), als...

AI Technical Summary

Problems solved by technology

The above methods for detecting NT-proBNP have the following disadvantages: 1) The gold calibration test is fast, but due to the low sensitivity of this method, the change of NT-proBNP content cannot be dynamically observed; There are high requirements for personnel; 3) The ELISA method uses horseradish peroxidase (horseradish peroxidase, abbreviated as HRP) or alkaline

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