GPA2 gene controlling seeds per ear of plant and applications thereof

A technology of grain number per spike and gene, applied in the field of GPA2 gene controlling the number of spikelets of plants, can solve the problems of the number of spikelets and high-level branches, low yield, etc.

Active Publication Date: 2014-06-25
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with cultivated rice, the number of grains per panicle and the number of high-level b

Method used

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  • GPA2 gene controlling seeds per ear of plant and applications thereof
  • GPA2 gene controlling seeds per ear of plant and applications thereof
  • GPA2 gene controlling seeds per ear of plant and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1, the discovery of controlling grain number per ear gene GPA2

[0033] One of the introgression lines, NIL92, was found from a set of introgression lines constructed by using the common wild rice in Yuanjiang, Yunnan as the donor parent and the excellent cultivated rice variety Teqing as the recipient parent. The number of secondary branches and grains per panicle of the introgressed segment of Oryza sativa in Yuanjiang was significantly lower than that of the recurrent parent Teqing. Introduce NIL92 and Teqing ( figure 1 ) backcross and then selfcross to construct F 2 The population was identified for genotype and phenotype, and a QTL related to the number of grains per panicle was detected at the end of the long arm of the second chromosome, named q-GPA2, and the allele from NIL92 was completely dominant relative to the allele from Teqing of. The genetic linkage map of the GPA2 region was constructed using the above population, and the target QTL was pr...

Embodiment 2

[0034] Embodiment 2, the acquisition of GPA2 transgenic rice

[0035] 1. Construction of Interference Fragments

[0036] 1. Obtaining DNA Fragment A and DNA Fragment B

[0037] (1) Design primers

[0038] Recognition sites for restriction endonucleases BamHI, KpnI, SpeI and SacI were respectively introduced at both ends of the primers. The primer sequences are as follows:

[0039] CRI1-F (Forward): 5'CC GGATCC CTCCTCTGCATTGTCTTCAG-3'The underlined base is the recognition site of the restriction endonuclease BamHI;

[0040] CRI1-R (reverse primer): 5'GG GGTACC ATGGGATTTACGGTGATTGA-3'underlined base is the recognition site of restriction endonuclease KpnI;

[0041] CRI2-F:5'TG ACTAGT GCTACGCCACTGCATAAG-3'The underlined base is the recognition site of restriction endonuclease SpeI;

[0042] CRI2R:5'TA GAGCTC GTTACGGCCTTCTTCCAAAT-3'The underlined base is the restriction endonuclease SacI recognition site;

[0043](2) Obtaining DNA Fragment A and DNA Fragment B

[004...

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Abstract

The invention discloses a GPS2 gene controlling seeds per ear of plant and applications thereof. A protein is shown as a) or b): a) a protein the amino acid sequence of which is shown as the sequence 1 in the sequence table; and b) a protein which is derived from the sequence 1, controls the seeds per ear, and is formed by the substitution and/or deletion and/or addition of one or more amino acid residues in the amino acid sequence shown in the sequence 1. Experiments show that: the protein and the encoding gene thereof are related with the formation of the seeds per ear, can be used for molecule mechanism research of formation of rice secondary rachis branches and for recapitulation of a rice domestication process, and has great significance and practical meaning for breeding of a high-output rice variety.

Description

technical field [0001] The invention relates to a GPA2 gene for controlling the number of grains per spike in plants and its application. Background technique [0002] Rice is one of the most important food crops in my country, and more than half of the country's population uses rice as a daily staple food. Therefore, in today's situation where China's population is increasing and the area of ​​arable land is decreasing, increasing the rice yield per unit area has become an effective means to satisfy the people's food and clothing and ensure national food security. The yield of rice consists of three elements: the number of panicles per unit area, the number of grains per panicle and the weight of thousand grains. Among them, the number of grains per panicle is considered to be the yield factor with the greatest growth potential due to the largest variation among different rice varieties. [0003] Common wild rice is the direct ancestor of cultivated rice. Since ancient h...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N5/10C12N15/113C12N15/82A01H5/00
CPCC07K14/415C12N15/113C12N15/8261C12N2310/141
Inventor 孙传清陈昊谭禄宾刘凤霞朱作峰付永彩才宏伟顾凭
Owner CHINA AGRI UNIV
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