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Application of lactobacillus casei grx12 in preparation of product for treating chronic alcoholic liver injury

A technology of alcoholic liver injury and Lactobacillus casei, which is applied in the field of microorganisms, can solve the problems of unrevealed protective effect of chronic alcoholic liver injury, and achieve the effects of alleviating liver inflammation, reducing blood lipids, and reducing expression

Inactive Publication Date: 2014-07-02
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention isolates a patented probiotic bacterium with significant in vitro antioxidant activity—Lactobacillus casei grx12—from the intestines of people in Changshou Village, Rugao, Jiangsu; this strain has been disclosed in the Chinese patent application No. Protective effect on liver injury

Method used

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  • Application of lactobacillus casei grx12 in preparation of product for treating chronic alcoholic liver injury
  • Application of lactobacillus casei grx12 in preparation of product for treating chronic alcoholic liver injury
  • Application of lactobacillus casei grx12 in preparation of product for treating chronic alcoholic liver injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] The original strain of Lactobacillus casei grx12 was inoculated into 12% skimmed milk (sterilized at 115°C for 20 minutes), cultured at 37°C for 18-24 hours to curd, and activated continuously for two generations as a mother starter. Inoculate the mother starter into 12% skimmed milk (sterilized at 115°C for 20 minutes) at 3% to 5% of the inoculum, and incubate at 37°C for 18-24 hours until the curd is reached. At this time, the number of viable bacteria can reach 10 9 ~10 10 cfu / mL, the starter of Lactobacillus casei grx12 is obtained.

Embodiment 2

[0067] Example 2: Using Lactobacillus casei grx12 and Streptococcus thermophilus grx02 to prepare functional fermented milk with protective effects on alcoholic liver injury

[0068] Streptococcus thermophilus grx02 is a patented strain obtained by the inventors with high antioxidant activity and protective function of alcoholic liver injury. The patent publication number is CN101328469A, and the strain deposit number is CGMCC No: 2525.

[0069] First, prepare starters of Lactobacillus casei grx12 and Streptococcus thermophilus grx02 respectively

[0070] Secondly, the pretreated milk is heated to about 60°C, 6% sucrose is added, and after it is fully dissolved, it is homogenized under a pressure of 20MPa. After heat treatment at 95℃ / 5min, it is cooled to about 42℃. Add 2.5% of the inoculum to the Lactobacillus casei grx12 starter prepared according to Example 1, and add 2.5% Streptococcus thermophilus grx02 starter, ferment to curd at 42°C, cool and refrigerate at 4°C to obtain Mi...

Embodiment 3

[0071] Example 3: Preparation of probiotic milk beverage using Lactobacillus casei grx12

[0072] The skim milk powder is made into 12% reconstituted skim milk 350kg, after heat treatment at 95℃ / 8~10min, it is cooled to 37~40℃. Inoculate the starter of Lactobacillus casei grx12 prepared according to Example 1 at 3%~5% inoculation amount, culture at 37℃ for 20~24h, and control the end point acidity at 155~170℃; add 650kg at 90~110℃ / 5~ A mixture of 10s sterilized sugar and stabilizer (the composition of the mixture is 0.9%~1.4% pectin and 13.8%~15.4% sucrose), mix well and homogenize at 20~25MPa, and then at 72℃ / 15s Heat treatment, cool to 15-20°C, and use aseptic or hygienic filling to obtain 1000kg of Lactobacillus casei grx12 milk beverage.

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Abstract

The invention relates to an application of anthropogenic lactobacillus casei grx12 in the aspect of chronic alcoholic liver injury. The lactobacillus casei grx12 is a probiotic strain with an obvious in-vitro antioxidant capacity, which is obtained in a separation mode from the intestinal tracts of people in the long life village of Rugao city, Jiangsu province. The lactobacillus casei grx12 is preserved in China General Microbiological Culture Collection Center (CGMCC) of China Committee for Culture Collection of Microorganisms (CCCCM) and has the preservation number of CGMCC No.7696. The lactobacillus casei grx12 can be used for obviously decreasing the level of toxins in blood fat and blood serum of a rat with the alcoholic liver injury, decreasing the expression of liver inflammatory factors in the liver and relieving the inflammatory reaction of the liver, thereby having obvious protection functions to the chronic alcoholic liver injury.

Description

Technical field [0001] The present invention relates to the technical field of microorganisms, in particular to the application of a human-derived Lactobacillus casei grx12 with protective effect on chronic alcoholic liver injury in fermented dairy products, functional foods and medicines. technical background [0002] Chronic liver disease caused by long-term heavy drinking is one of the important causes of liver damage in humans. In our country, with the improvement of people’s living standards, alcoholics have increased, and chronic alcoholism has become the cause of liver damage following viral hepatitis. The second leading cause. Alcoholic liver injury usually manifests as fatty liver in the early stage, which can then develop into alcoholic hepatitis, alcoholic liver fibrosis and alcoholic cirrhosis. Therefore, it is very important to explore the pathogenesis of alcoholic liver disease and preventive intervention measures. [0003] The pathogenesis of alcoholic injury is mo...

Claims

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Application Information

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IPC IPC(8): A61K35/74A61P1/16A23C9/12A23G9/40A61K35/747
Inventor 顾瑞霞刘东黄玉军陈霞陈大卫郭飞翔赵堂彦蒋欣荣
Owner YANGZHOU UNIV
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