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Perylene grafted poly (4-vinylpyridine), synthesis thereof and preparation of fluorescent probe

A vinylpyridine, fluorescent probe technology, applied in the synthesis of fluorescent polyelectrolyte, fluorescent probe field, can solve the problem of not high fluorescence energy resonance transfer efficiency, affecting product fluorescence efficiency and test results, etc., to achieve product fluorescence energy resonance Effects of improved transfer efficiency and improved fluorescence efficiency

Inactive Publication Date: 2014-07-02
UNIV OF SCI & TECH BEIJING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The fluorescence energy resonance transfer efficiency of the above experiment is not high enough, which affects the fluorescence efficiency and test results of the product

Method used

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  • Perylene grafted poly (4-vinylpyridine), synthesis thereof and preparation of fluorescent probe
  • Perylene grafted poly (4-vinylpyridine), synthesis thereof and preparation of fluorescent probe
  • Perylene grafted poly (4-vinylpyridine), synthesis thereof and preparation of fluorescent probe

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Experimental program
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Embodiment 1

[0030] Synthesis of a perylene-grafted poly(4-vinylpyridine): Add 5 mmol of poly(4-vinylpyridine) with a weight-average molecular weight of 60,000 into a three-necked flask, add 15 mL of chloroform to dissolve, and then add 0.2mmol of perylene bromide, under nitrogen protection, condensed and refluxed at 68°C for about 24 hours. After the reaction, the reaction solution was slowly added dropwise into a large amount of tetrahydrofuran to obtain a precipitate, which was repeatedly dissolved and precipitated, and then vacuum-dried to obtain PFP. Dissolve PFP in an appropriate amount of absolute ethanol, add a large amount of iodomethane, protect with nitrogen, and stir and reflux at 40°C for 24 hours. After the reaction is finished, the reaction solution is vacuum rotary distilled to obtain a solid powder product, which is further vacuum dried to obtain the final product PFPM. The grafting rate of perylene in the finally measured PFPM is 2.5%, and the weight average molecular we...

Embodiment 2

[0038]Synthesis of a perylene-grafted poly(4-vinylpyridine): Add 5 mmol of poly(4-vinylpyridine) with a weight-average molecular weight of 60,000 into a three-necked flask, add 15 mL of chloroform to dissolve, and then add 0.2mmol of perylene bromide, under nitrogen protection, condensed and refluxed at 68°C for about 24 hours. After the reaction, the reaction solution was slowly added dropwise into a large amount of tetrahydrofuran to obtain a precipitate, which was repeatedly dissolved and precipitated, and then vacuum-dried to obtain PFP. Dissolve PFP in an appropriate amount of absolute ethanol, add a large amount of iodomethane, protect with nitrogen, and stir and reflux at 40°C for 24 hours. After the reaction is finished, the reaction solution is vacuum rotary distilled to obtain a solid powder product, which is further vacuum dried to obtain the final product PFPM. The grafting rate of perylene in the finally measured PFPM is 2.5%, and the weight average molecular wei...

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Abstract

The invention relates to perylene grafted poly (4-vinylpyridine), synthesis thereof and preparation of a fluorescent probe, and belongs to the fields of functional polymer technology, analytical chemistry and bioanalytical chemistry. The synthesis method comprises the steps of: grafting perylene A bromine onto poly (4-vinylpyridine); protonizing the product by using iodomethane to obtain perylene grafted poly (4-vinylpyridine) PFPM cationic polyelectrolyte. The invention further discloses a method for preparing the fluorescent probe by using perylene grafted poly (4-vinylpyridine). The synthesis method of the dis-conjugated polyelectrolyte PFPM disclosed by the invention is easy to operate; when DNA is added in the PFPM solution to form a composite probe, PFPM is aggregated, and the fluorescence intensity is increased. According to the invention, a dual detection mechanism of intercalation and aggregation-induced emission is provided, is not previously reported and has good reference meaning to future researches.

Description

technical field [0001] The invention belongs to the fields of functional macromolecule technology, analytical chemistry and bioanalytical chemistry, and relates to a method for synthesizing a water-soluble fluorescent polyelectrolyte with aggregation-induced luminescence properties and a method for preparing a fluorescent probe for detecting oligonucleotide sequences by using the polyelectrolyte method. [0002] Background technique [0003] Functional polymers are polymers with specific functions formed by combining specific functional groups in their macromolecular chains, or compounding macromolecules with other materials with specific functions, or both. Materials, and its application in the field of bioanalytical chemistry, especially in the detection of biomacromolecules, has attracted close attention from scholars at home and abroad. Using functional polymers with fluorescent properties as fluorescent probes to specifically recognize nucleic acids has become a curre...

Claims

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Application Information

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IPC IPC(8): C08F126/06C08F8/44C09K11/06G01N21/64C12Q1/68
Inventor 王国杰张瑞辰许琛周茹意
Owner UNIV OF SCI & TECH BEIJING
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