Microfluidic microbial culture chip
A microbial culture and microfluidic technology, applied in the field of microfluidics, can solve the problems of increasing the difficulty of chip alignment and packaging, unsuitable for culturing microorganisms in suspension, affecting the dissolved oxygen content of pipelines, etc., to expand the variety and application range, reduce Production difficulty and the effect of saving chip area
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Embodiment 2
[0057] Embodiment 2 is the same as Embodiment 1 in terms of principles and working relationships, and details are not repeated here.
[0058] image 3 Shown is a schematic structural view of the microfluidic microorganism culture chip in the third embodiment of the present invention.
[0059] ginseng image 3 As shown, the microfluidic microbial culture chip includes a culture unit, and each culture unit includes a ring-shaped closed pipe 3101 located in the liquid flow layer, except that the width of the two branch pipes of the ring-shaped closed pipe 3101 is 50 microns, the rest The channel widths were all 100 μm. Both ends of the annular closed pipe 3101 communicate with the outside world. The air supply pipeline 3202 and the circulation drive channel 3201 are distributed in the pneumatic control layer of the chip. The air supply pipeline 3202 is a pipeline with branches, which passes through the ring-shaped closed pipeline 3101 multiple times, and the depth of all pipel...
Embodiment 3
[0060] Embodiment 3 is the same as Embodiment 1 in terms of principle and working relationship, and will not be repeated here.
[0061] Figure 4 Shown is a schematic structural view of the microfluidic microorganism culture chip in the fourth embodiment of the present invention.
[0062] ginseng Figure 4 As shown, the microfluidic microbial culture chip includes a plurality of culture units, and each culture unit includes a ring-shaped closed pipeline 4101 located in the liquid flow layer. The channel widths were all 100 μm. Both ends of the annular closed pipe 4101 communicate with the outside world. The air supply pipeline 4202 and the circulation drive channel 4201 are distributed in the pneumatic control layer of the chip, both of which are 100 microns in width. The depth is 10 microns. The pneumatic control layer and the liquid flow layer are separated by an elastic air-permeable film, and the thickness of the elastic air-permeable film is 20 microns. Each layer i...
Embodiment 4
[0063] Embodiment 4 is the same as Embodiment 1 in terms of principle and working relationship, so details are not repeated here.
[0064] Figure 5 Shown is a schematic structural view of the microfluidic microorganism culture chip in the fifth embodiment of the present invention.
[0065] ginseng Figure 5As shown, the microfluidic microbial culture chip includes a plurality of culture units, and each culture unit includes a ring-shaped closed pipeline 5101 located in the liquid flow layer. The channel widths were all 100 μm. Both ends of the annular closed pipe 5101 communicate with the outside world. The air supply pipeline 5202 and the circulation drive channel 5201 are distributed in the pneumatic control layer of the chip, both of which are 100 microns in width. The air supply pipeline 5202 is a pipeline bent multiple times, passing through different ring-shaped closed pipelines 5101 with different crossing times. Above, all ducts are 10 microns deep. The pneumatic...
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