A biological synergic microbial agent and applications thereof

A technology of biological synergy and inoculum, applied in the directions of microorganisms, sustainable biological treatment, biological water/sewage treatment, etc., can solve the problems of high equipment cost and low COD degradation rate, so as to reduce operating costs and improve sludge structure. Effects of composition and biological activity

Active Publication Date: 2014-07-02
中国中化股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention is committed to developing a biosynergistic bacterial agent to solve the prob

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: the acquisition of biological synergistic bacterial classification

[0030] The biostimulant bacterial strain SY-NPD-3 was isolated from the sludge of a coking plant in Anshan City, Liaoning Province. It was identified as Pseudomonas by 16S rDNA gene sequencing. General Microbiology Center of Species Preservation Committee, the deposit number is CGMCC No.6823. The screening medium of bacterial strain SY-NPD-3 consists of: 2000ppm naphthalene, 400ppm (NH 4 ) 2 SO 4 , Agar 15g / L. The sludge was serially diluted (diluted 10 6 、10 7 、10 8 times), pipette 0.1mL dilution and add it to the screening medium, spread it evenly with a glass rod, incubate at 30°C for 2-4 days, pick the fastest-growing strain and name it SY-NPD-3, and amplify its 16S rDNA And its 16S rDNA sequence was determined, and it was confirmed as Pseudomonas after comparison in GenBank.

[0031] The biosynthetic bacterial strain SY-PD-27 was isolated from the sludge of a coking plant in ...

Embodiment 2

[0035] Embodiment 2: the preparation of biological synergistic bacterial agent 1

[0036] Pseudomonas sp., CGMCC No.6823) SY-NPD-3, Acinetobacter sp., CGMCC No.6824) SY-PD-27, Bordetella sp., CGMCC No.6825) SY-PDD-9, Klebsiella sp. (CGMCC No.6826) SY-SW51, Bacillus magterium (CGMCC No.5225) SY-Z5, Bacillus subtilis ( Bacillus subtilis, CGMCC No.5224) The six strains of SY-ND were inoculated into sterilized LB medium for activation as seed liquid, and cultured at 30°C for 24 hours. At this time, the concentration of the fermentation liquid of each strain could reach 10 9 cfu / mL or more. Then, the seed solution of the 6 strains was inserted into the sterilized LB medium with an inoculum amount of 1%-3% (the volume of the seed solution to the volume of the fermentation medium) for fermentation, and cultured at 30°C for 24h-36h to make the bacteria The body concentration reaches 10 9 —10 11 cfu / mL. The fermentation liquid of the above 6 strains was prepared into a mixed solut...

Embodiment 3

[0037] Embodiment 3: the preparation 2 of biological synergistic bacterial agent

[0038] The method is the same as that in Example 2, except that the fermentation liquid of the above-mentioned 6 strains is prepared into a mixed liquid according to the volume of 40:5:15:18:18:18.

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Abstract

The invention relates to a biological synergic microbial agent SY-CD-B and applications thereof in coking wastewater treatment. The biological synergic microbial agent is prepared by mixing six screened bacteria that are pseudomonas sp., acinetobacter sp., bordetella sp., klebsiella sp., bacillus magterium and bacillus subtilis according to a fermentation broth volume ratio of 5-50:1-5:3-15:2-20:2-20:2-20, centrifuging and diluting the bacteria with an inorganic salt culture liquid. By utilization of the biological synergic microbial agent and by combining a mixed type active sludge system, the COD concentration of biochemical treatment effluent is reduced, and no extra equipment investment is added. The biological synergic microbial agent is suitable for biochemical treatment of coking wastewater of water of various kinds of quality, and largely reduces the treatment cost.

Description

technical field [0001] The invention belongs to the field of coking wastewater treatment, and relates to a biologically synergistic bacterial agent for treating coking wastewater and its application. technical background [0002] Bioaugmentation is a method of enhancing the effect of the original microbial population by adding microbial strains with a specific degradation ability. Special-effect microorganisms are dominant strains screened from nature, without any adverse effects on the original biochemical system. This method does not replace the existing bacterial flora, but it can improve the response of the flora to certain specific flora under specific circumstances, or enhance the ability of the flora to degrade sewage components, thereby improving the sewage treatment effect. Compared with physical and chemical methods, biosynthesis has the following advantages: no additional equipment is required, cost savings, targeted removal for different water quality characteri...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C02F3/12C12R1/38C12R1/22C12R1/11C12R1/125C12R1/01
CPCY02W10/10
Inventor 朱希坤李小明王芳戴速航孙鸿曼
Owner 中国中化股份有限公司
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