A biological synergic microbial agent and applications thereof
A technology of biological synergy and inoculum, applied in the directions of microorganisms, sustainable biological treatment, biological water/sewage treatment, etc., can solve the problems of high equipment cost and low COD degradation rate, so as to reduce operating costs and improve sludge structure. Effects of composition and biological activity
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Embodiment 1
[0029] Embodiment 1: the acquisition of biological synergistic bacterial classification
[0030] The biostimulant bacterial strain SY-NPD-3 was isolated from the sludge of a coking plant in Anshan City, Liaoning Province. It was identified as Pseudomonas by 16S rDNA gene sequencing. General Microbiology Center of Species Preservation Committee, the deposit number is CGMCC No.6823. The screening medium of bacterial strain SY-NPD-3 consists of: 2000ppm naphthalene, 400ppm (NH 4 ) 2 SO 4 , Agar 15g / L. The sludge was serially diluted (diluted 10 6 、10 7 、10 8 times), pipette 0.1mL dilution and add it to the screening medium, spread it evenly with a glass rod, incubate at 30°C for 2-4 days, pick the fastest-growing strain and name it SY-NPD-3, and amplify its 16S rDNA And its 16S rDNA sequence was determined, and it was confirmed as Pseudomonas after comparison in GenBank.
[0031] The biosynthetic bacterial strain SY-PD-27 was isolated from the sludge of a coking plant in ...
Embodiment 2
[0035] Embodiment 2: the preparation of biological synergistic bacterial agent 1
[0036] Pseudomonas sp., CGMCC No.6823) SY-NPD-3, Acinetobacter sp., CGMCC No.6824) SY-PD-27, Bordetella sp., CGMCC No.6825) SY-PDD-9, Klebsiella sp. (CGMCC No.6826) SY-SW51, Bacillus magterium (CGMCC No.5225) SY-Z5, Bacillus subtilis ( Bacillus subtilis, CGMCC No.5224) The six strains of SY-ND were inoculated into sterilized LB medium for activation as seed liquid, and cultured at 30°C for 24 hours. At this time, the concentration of the fermentation liquid of each strain could reach 10 9 cfu / mL or more. Then, the seed solution of the 6 strains was inserted into the sterilized LB medium with an inoculum amount of 1%-3% (the volume of the seed solution to the volume of the fermentation medium) for fermentation, and cultured at 30°C for 24h-36h to make the bacteria The body concentration reaches 10 9 —10 11 cfu / mL. The fermentation liquid of the above 6 strains was prepared into a mixed solut...
Embodiment 3
[0037] Embodiment 3: the preparation 2 of biological synergistic bacterial agent
[0038] The method is the same as that in Example 2, except that the fermentation liquid of the above-mentioned 6 strains is prepared into a mixed liquid according to the volume of 40:5:15:18:18:18.
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