Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of biological synergist and its application

A technology of biological synergy and inoculum, applied in microorganisms, sustainable biological treatment, biological water/sewage treatment, etc., can solve the problems of high equipment cost and low COD degradation rate, so as to reduce operating costs and improve sludge structure. Effects of composition and biological activity

Active Publication Date: 2015-12-23
中国中化股份有限公司 +1
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention is committed to developing a biosynergistic bacterial agent to solve the problems of high equipment cost and low COD degradation rate in the existing coking wastewater treatment method

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: the acquisition of biological synergistic bacterial classification

[0030] The biostimulant strain SY-NPD-3 was isolated from the sludge of a coking plant in Anshan City, Liaoning Province. It was identified as Pseudomonas by 16SrDNA gene sequencing and was deposited in China Microorganisms on November 13, 2012. General Microbiology Center of the Preservation Management Committee, the deposit number is CGMCCNo.6823. The screening medium of bacterial strain SY-NPD-3 consists of: 2000ppm naphthalene, 400ppm (NH 4 ) 2 SO 4 , Agar 15g / L. The sludge was serially diluted (diluted 10 6 、10 7 、10 8 times), pipette 0.1mL dilution solution and add it to the screening medium, spread it evenly with a glass rod, incubate at 30°C for 2-4 days, pick the fastest growing strain and name it SY-NPD-3, amplify its 16SrDNA and Its 16SrDNA sequence was determined, and it was identified as Pseudomonas after comparison in GenBank.

[0031] The biosynthetic bacterial stra...

Embodiment 2

[0035] Embodiment 2: the preparation of biological synergistic bacterial agent 1

[0036] Pseudomonas sp., CGMCC No.6823) SY-NPD-3, Acinetobacter sp., CGMCC No.6824) SY-PD-27, Bordetella sp., CGMCC No.6825) SY -PDD-9, Klebsiella sp. (CGMCC No.6826) SY-SW51, Bacillus magterium (CGMCC No.5225) SY-Z5, Bacillus subtilis (CGMCC No.5224) SY-ND The 6 strains were inoculated into sterilized LB medium for activation as seed liquid, and cultured at 30°C for 24 hours. At this time, the concentration of the fermentation liquid of each strain could reach 10 9 cfu / mL or more. Then, the seed solution of the 6 strains was inserted into the sterilized LB medium with an inoculum amount of 1%-3% (the volume of the seed solution to the volume of the fermentation medium) for fermentation, and cultured at 30°C for 24h-36h to make the bacteria The body concentration reaches 10 9 —10 11 cfu / mL. The fermentation liquid of the above 6 strains was prepared into a mixed solution according to the vol...

Embodiment 3

[0037] Embodiment 3: the preparation 2 of biological synergistic bacterial agent

[0038] The method is the same as that in Example 2, except that the fermentation liquid of the above-mentioned 6 strains is prepared into a mixed liquid according to the volume of 40:5:15:18:18:18.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a biological synergic microbial agent SY-CD-B and applications thereof in coking wastewater treatment. The biological synergic microbial agent is prepared by mixing six screened bacteria that are pseudomonas sp., acinetobacter sp., bordetella sp., klebsiella sp., bacillus magterium and bacillus subtilis according to a fermentation broth volume ratio of 5-50:1-5:3-15:2-20:2-20:2-20, centrifuging and diluting the bacteria with an inorganic salt culture liquid. By utilization of the biological synergic microbial agent and by combining a mixed type active sludge system, the COD concentration of biochemical treatment effluent is reduced, and no extra equipment investment is added. The biological synergic microbial agent is suitable for biochemical treatment of coking wastewater of water of various kinds of quality, and largely reduces the treatment cost.

Description

technical field [0001] The invention belongs to the field of coking wastewater treatment, and relates to a biologically synergistic bacterial agent for treating coking wastewater and its application. technical background [0002] Bioaugmentation is a method of enhancing the effect of the original microbial population by adding microbial strains with a specific degradation ability. Special-effect microorganisms are dominant strains screened from nature, without any adverse effects on the original biochemical system. This method does not replace the existing bacterial flora, but it can improve the response of the flora to certain specific flora under specific circumstances, or enhance the ability of the flora to degrade sewage components, thereby improving the sewage treatment effect. Compared with physical and chemical methods, biosynthesis has the following advantages: no additional equipment is required, cost savings, targeted removal for different water quality characteri...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C02F3/12C12R1/38C12R1/22C12R1/11C12R1/125C12R1/01
CPCY02W10/10
Inventor 朱希坤李小明王芳戴速航孙鸿曼
Owner 中国中化股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com