Method for preparing fatty acid colloid solution applied to crypthecodinium cohnii for producing docosahexaenoic acid

A technology of docosahexaenoic acid and colloidal solution, which is applied in the field of preparation of fatty acid colloidal solution by artificial emulsification, can solve problems such as innovative applications that have not been published or reported, achieve flexible addition methods, improve utilization, The effect of low preparation cost

Active Publication Date: 2014-07-02
INST OF PLASMA PHYSICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no publication or report on the practical technology of the innovative application of colloidal biological effects in artificial bioreactors and artificial culture conditions.

Method used

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  • Method for preparing fatty acid colloid solution applied to crypthecodinium cohnii for producing docosahexaenoic acid
  • Method for preparing fatty acid colloid solution applied to crypthecodinium cohnii for producing docosahexaenoic acid
  • Method for preparing fatty acid colloid solution applied to crypthecodinium cohnii for producing docosahexaenoic acid

Examples

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Effect test

Embodiment 1

[0019] Emulsified palmitic acid as fermentation additive

[0020] Palmitic acid (molecular formula: C 16 h 32 o 2 ) at room temperature is a white scaly solid with pearly luster, insoluble in water, directly added as a substrate is not easily absorbed by microalgae, and is also a precursor for the synthesis of docosahexaenoic acid (DHA).

[0021] Take 500 milliliters of water, add 25 grams of solid palmitic acid, and then add 8 milliliters of Tween80 reagent. Low concentration of additives will not achieve emulsification effect, and high concentration will also cause instability of the colloidal system. Slowly heat to 90°C, use a general-purpose ultrasonic generator device, the diameter of the horn is about 6mm, put an appropriate amount of glass beads (5 grams) at the bottom of the shaking container to assist emulsification, do not let the horn directly contact with the wall of the container, The rod is 20-30mm away from the bottom of the container, and the power of the u...

Embodiment 2

[0038] Use emulsified oleic acid as a fermentation additive

[0039] Oleic acid (molecular formula: C 18 h 34 o 2 ) Colorless oily liquid at room temperature (melting point 16.3°C), insoluble in water.

[0040] Take 500 ml of water, add 25 grams of liquid oleic acid, and then add 5 ml of rhamnosyl ester (food grade). Slowly heat to 90°C, use a general-purpose ultrasonic generator device, the diameter of the horn is about 6mm, put an appropriate amount of glass beads (5 grams) at the bottom of the shaking container to assist emulsification, do not let the horn directly contact with the wall of the container, The rod is 20-30mm from the bottom of the container, and the power of the ultrasonic transducer is set to 130W. During the cavitation process, the thermal effect causes the temperature to be too high to make the system boil, and emulsifies in short time intervals (working for 2 seconds and 2 seconds apart). Ultrasonic vibration treatment for 1 minute, the control tempe...

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Abstract

The invention discloses a method for preparing a fatty acid colloid solution applied to crypthecodinium cohnii for producing a docosahexaenoic acid. According to the method, the fatty acid colloid solution is artificially prepared and is applied to the heterotrophic culture of the crypthecodinium cohnii, so that the yield of a polyunsaturated fatty acid is obviously increased. The method comprises the steps that the ingredients of a fatty acid precursor are treated by adopting a specific ultrasonic emulsification method so as to form micro-nano granules dispersed into a culture solution, wherein the fatty acid stably exists in the solution and is not layered, accumulated or precipitated, and is supplied into a culture system; and after the crypthecodinium cohnii is continuously fermented for multiple days, the accumulation speed of cultured microalgae grease and the ratio of a long-chain fatty acid are obviously expedited and improved. The technical method is easy to implement and suitable for the artificial culture of heterotrophic microalgae.

Description

technical field [0001] The invention relates to a heterotrophic microalgae Cryptodinium algae capable of synthesizing docosahexaenoic acid, in particular to a method for preparing a colloidal solution of fatty acids by artificial emulsification, which uses the microalgae and adds artificial colloidal solution to produce polyunsaturated Methods of production of fatty acids. Background technique [0002] Docosahexaenoic acid (DHA) is a kind of polyunsaturated fatty acids (PUFAs), which is an important component of phospholipids in animal cell membranes and determines the fluidity and deformability of cell membranes. It plays an important role in the prevention and treatment of various diseases and certain cancers, and the intake of sufficient PUFAs is crucial to the development of vision and nerves of fetuses and young children. European and American countries now have a wide range of applications for DHA, mainly in food and health care medicines. Applications in food includ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/89
Inventor 姚黎明肖尚陈祥松吴金勇姚建铭
Owner INST OF PLASMA PHYSICS CHINESE ACAD OF SCI
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