Method of preparing natural milk-flavored essence by virtue of enzymatic hydrolysis of double enzymes and fermentation of multiple microorganisms

A technology of microbial fermentation and double-enzyme enzymatic hydrolysis, which is applied in the field of flavor preparation, can solve the problems of low fermentation efficiency, bad flavor, and low acid production, so as to achieve rich and rich milk flavor, improve flavor and taste, and enhance the sense of richness. Effect

Active Publication Date: 2014-07-09
I&D INT FLAVOURS & FRAGRANCES GZ
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  • Abstract
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Problems solved by technology

[0007] Dual-enzyme enzymatic hydrolysis is to use lipase to enzymolyze fat to form pure oil and fat smell, and at the same time to use protease to hydrolyze milk protein to form a strong taste peptide, which forms a synergistic effect with the bitter and waxy taste caused by long-chain fatty acids, thereby eliminating bad taste Flavor; add Lactobacillus to produce a large amount of lactic acid and consume oxygen in raw materials at the same time to provide an anaerobic environment for Lactobacillus diacetylactis and Lactobacillus bulgaricus to promote fermentation and overcome the low fermentation efficiency and time caused by the use of anaerobic fermentation alone Disadvantages such as long length and low acid production

Method used

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Embodiment Construction

[0029] The specific operation steps of the present invention are described below in conjunction with specific embodiments, but the present invention is not limited to this embodiment:

[0030] a. Melting of raw cream: Melt bright cream at 30℃;

[0031] b. Add non-aqueous medium: use acetone as the medium and add 5 parts of acetone to 90 parts of cream;

[0032] c. Add emulsifier: add 0.1% monoglyceride;

[0033] d. Homogeneous emulsification: the homogenizer rotates at 10,000rpm and the emulsification time is 3min;

[0034] e. Pasteurization: 85°C for 5min;

[0035] f. Cooling down: cooling down to 50°C;

[0036] g. Double enzyme digestion: add Novozymes lipase Palatase 20,000L and Novozymes compound flavor protease at the same time for enzymolysis

[0037] g.1 Add enzyme amount: lipase is 430U / g substrate, protease is 330U / g substrate,

[0038] g.2 The temperature is 45℃,

[0039] g.3 Adjust the pH to 7.5,

[0040] g.4 Enzymolysis time 4h;

[0041] h. Enzyme inactivation: 15min at 85℃...

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Abstract

The invention discloses a method of preparing natural milk-flavored essence by virtue of enzymatic hydrolysis of double enzymes and fermentation of multiple microorganisms, with natural cream as a raw material. The method comprises the following operating steps of melting the cream; adding a medium and an emulsifier; carrying out homogeneous emulsification; carrying out pasteuring; carrying out enzymatic hydrolysis of the double enzymes, anddeactivating the double enzymes; carrying out fermentation of the microorganisms, and drying to prepare the milk-flavored essence. With the adoption of the method, the enzymatic hydrolysis time is shortened; middle-and-short-chain fatty acids of C4-C12 are generated; meanwhile, as milk proteins are hydrolyzed into flavor such as polypeptide and amino acid by virtue of protease to form a unique milk flavor, the strong, pleasure and lasting feelings of the taste of the milk in the essence are strengthened, and the bitterness and the wax taste caused by the middle-and-short-chain fatty acids are eliminated; by virtue of the fermentation of multiple microorganisms, flavor compounds are increased; as a great deal of lactic acids are generated due to the fermentation of lactic acid bacillus, the flavor and the taste can be improved to form diversified acides and milk flavors, and the fragrance is further softened. The natural milk-flavored essence prepared according to the invention is pure and soft in fragrance and is strong in milk flavor; and compared with the natural cream without treatment, the fragrance of the natural milk-flavored essence can be strengthened by 20-300 times.

Description

Technical field [0001] The invention relates to a preparation method of flavor, in particular to a method for preparing natural milk flavor flavor by double-enzyme enzymolysis and fermentation of various microorganisms, belonging to the field of food processing. Background technique [0002] Food flavors and fragrances are a kind of food and beverage additives. It has a wide range of applications and can be said to be developed in parallel with the food and beverage industries. With the continuous improvement of people's living standards and the continuous improvement of dietary structure, the industry of flavors and fragrances has been continuously developed, the quality of products has been continuously improved, the variety has been continuously increased, and the output and sales have increased year by year. [0003] Boswellia flavor is a flavor with frankincense aroma. It is one of the flavors with the largest investment in the field of flavors and fragrances and the most wid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/23A23L27/24
CPCA23L27/25A23V2002/00A23V2400/119A23V2400/123A23V2400/11A23V2200/16A23V2200/14A23V2300/28
Inventor 武学文李建明李俊杰张裕文
Owner I&D INT FLAVOURS & FRAGRANCES GZ
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