Liquid fermentation method of ginseng-radix astragali-lucid ganoderma medical mycoplasm

A technology of liquid fermentation and medicinal bacteria, applied in the direction of microorganism-based methods, biochemical equipment and methods, fungi, etc.

Active Publication Date: 2014-07-09
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

At present, there is no document disclosing the two-way liquid fermentation process of using Ganoderma lucidum as the fermentation strain and fermenting the compoun

Method used

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  • Liquid fermentation method of ginseng-radix astragali-lucid ganoderma medical mycoplasm
  • Liquid fermentation method of ginseng-radix astragali-lucid ganoderma medical mycoplasm
  • Liquid fermentation method of ginseng-radix astragali-lucid ganoderma medical mycoplasm

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Experimental program
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Effect test

Embodiment 1

[0024] Grind the traditional Chinese medicine ginseng and astragalus, pass through a 10-mesh sieve, weigh 5 parts of 30g ginseng, then weigh 6g, 10g, 15g, 30g, 60g, and 90g of astragalus, mix them with ginseng, add 500ml of distilled water, heat and reflux to extract twice , each time for 0.5h, combine the water extracts and dilute to 1L, pH is natural, divide into 250ml Erlenmeyer flasks, each bottle contains 50ml, seal, sterilize at 121°C for 20min, cool for later use. Inoculate the strains of red sesame and cold sesame stored in PDA solid medium into the liquid fermentation medium based on the water extract of the whole medicinal materials of Chinese medicine ginseng and astragalus, 2 pieces per bottle, 26°C, 150r / After culturing for 12 days under the condition of min, the fermentation liquid was filtered, washed three times repeatedly, filtered, and dried to obtain the medicinal mycelia of Shenqizhi. The mycelium quantity data that embodiment 1 obtains is as shown in tabl...

Embodiment 2

[0028] Grind ginseng and astragalus, which are traditional Chinese medicines, and pass through a 10-mesh sieve. Weigh 30g ginseng and 10g astragalus as the red sesame fermentation substrate; After 0.5h, the combined water extracts were adjusted to 1L, with a natural pH, and were divided into 250ml Erlenmeyer flasks, each containing 50ml of liquid. Seal, sterilize at 121°C for 20 minutes, and cool down for later use. Inoculate the strains of red sesame and cold sesame stored in PDA solid medium into 3mm×3mm liquid fermentation medium with water extract of whole medicinal materials of Chinese medicine ginseng and astragalus as the substrate, 2 pieces per bottle, and adjust the temperature to 22°C , 24°C, 26°C, 28°C, 30°C, and cultured for 12 days under the conditions of 150r / min, the fermentation broth was filtered, washed three times repeatedly, filtered, and dried to obtain the medicinal mycelium of Shenqi Zhizhi. The mycelium quantity data that embodiment 2 obtains is as sho...

Embodiment 3

[0033] Grind ginseng and astragalus, which are traditional Chinese medicines, and pass through a 10-mesh sieve. Weigh 30g ginseng and 10g astragalus as the red sesame fermentation substrate; After 0.5h, the combined water extracts were adjusted to 1L, with a natural pH, and were divided into 250ml Erlenmeyer flasks, each containing 50ml of liquid. Seal, sterilize at 121°C for 20 minutes, and cool down for later use. Inoculate the strains of red sesame and cold sesame stored in PDA solid medium into the liquid fermentation medium based on the water extract of the whole medicinal materials of traditional Chinese medicine ginseng and astragalus, 2 pieces per bottle, and adjust the speed to 100, 120, 140, 160, 180 r / min, cultured at 26°C for 12 days, the fermentation broth was filtered, washed three times repeatedly, filtered, and dried to obtain the medicinal mycelium of Shenqizhi. The mycelium quantity data that embodiment 3 obtains is as shown in table 4:

[0034] Table 4

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Abstract

The invention provides a liquid fermentation method of ginseng-radix astragali-lucid ganoderma medical mycoplasm, and belongs to the field of traditional Chinese medicine biological fermentation. According to the liquid fermentation method of ginseng-radix astragali-lucid ganoderma medical mycoplasm, a liquid fermentation culture medium is inoculated with lucid ganoderma strains preserved in PDA culture medium, wherein the liquid fermentation culture medium is made from ginseng and radix astragali water extracts; culturing is carried out at a temperature of 22 to 30 DEG C with a rotating speed of 100 to 180r/min for 10 to 20 days so as to obtain the ginseng-radix astragali-lucid ganoderma medical mycoplasm. The liquid fermentation culture medium is made from ginseng and radix astragali water extracts, no other chemical ingredient is added, so that the liquid fermentation culture medium is safe and harmless; ginseng and radix astragali are combined, so that drug property and effect deficiencies of ginseng and radix astragali are avoided, and application range is widened; and in addition, the obtained ginseng-radix astragali-lucid ganoderma medical mycoplasm contains active ingredients of ginseng, radix astragali, and lucid ganoderma because of lucid ganoderma fermentation, effective combination of the three traditional traditional Chinese medicine ingredients is realized, balance of drug property and effect is achieved, and biological activities of original medicinal materials are improved.

Description

technical field [0001] The invention belongs to the field of microbial fermentation of traditional Chinese medicines, and in particular relates to a method for liquid fermentation of ginseng medicinal fungus. Background technique [0002] Both ginseng and astragalus are commonly used qi invigorating medicines in clinical treatment, but there are differences between the two in terms of properties and efficacy. Ginseng is sweet and slightly bitter in taste and flat in nature, nourishing qi and nourishing yin, keeping it and not going away. Astragalus membranaceus is sweet in taste and warm in nature, invigorates qi and supports yang, and it does not stay when it goes away. Pairing the two, ginseng, one guards and the other goes away, takes into account both yin and yang, tonifies the inside and outside without diarrhea, can be used for all qi deficiency diseases, and has a powerful effect of nourishing qi and supporting energy. The medicinal fungus Ganoderma lucidum is a tra...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
Inventor 宋凤瑞苏玲陈维佳皮子凤邢俊鹏刘志强
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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