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Bacillus circulans and application thereof in preparation of galactooligosaccharide by symbiotic fermentation technology

A technology of Bacillus circulans and galacto-oligosaccharides, applied in fermentation, microorganism-based methods, bacteria, etc., to achieve the effect of improving utilization rate, saving investment in supporting facilities, and improving enzyme utilization rate

Active Publication Date: 2014-07-09
BAOLINGBAO BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the above problems in the production of galacto-oligosaccharides, the process route is longer, the available enzymes are lost, the comprehensive utilization rate of enzymes is low, and the energy consumption is high. The invention provides a strain of Bacillus circulans that can shorten the production process route, reduce equipment investment, and improve enzyme utilization efficiency

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] An application of bacillus circulans in the production of galacto-oligosaccharides by symbiotic fermentation technology, comprising the steps of:

[0045] (1) Bacteria expansion culture

[0046] Inoculate the slant of Bacillus circulans BLB-32 (CGMCC No.8864) in the seed medium of the shaker flask, and cultivate it at 30°C for 10 hours at a shaking speed of 250rpm to obtain the expanded strain culture medium; seed medium: glucose 5g / L, yeast extract 5g / L, peptone 5g / L, magnesium sulfate 0.3 g / L, pH 7.0, seed medium was sterilized at 121°C for 15 minutes;

[0047] (2) Enlarged culture for enzyme production

[0048] Enzyme production medium: lactose 5g / L, yeast extract 5g / L, peptone 5g / L, magnesium sulfate 0.3g / L, disodium hydrogen phosphate 0.01g / L, sodium dihydrogen phosphate 0.01g / L, pH7.0, Sterilize the enzyme culture medium at 121°C for 15 minutes;

[0049]Pour the expanded strain culture solution obtained in step (2) into a 15-liter fermenter filled with enzyme-...

Embodiment 2

[0055] An application of bacillus circulans in the production of galacto-oligosaccharides by symbiotic fermentation technology, comprising the steps of:

[0056] (1) Bacteria expansion culture

[0057] Inoculate the slant of Bacillus circulans BLB-32 (CGMCC No.8864) in the seed medium of the shake flask, and cultivate it at 35°C for 24 hours at a shaking speed of 100rpm to obtain the expanded culture medium; seed medium: glucose 20g / L, yeast extract 20g / L, peptone 20g / L, magnesium sulfate 0.3 g / L, pH 7.0, seed medium was sterilized at 121°C for 15 minutes;

[0058] (2) Enlarged culture for enzyme production

[0059] Enzyme production medium: lactose 20g / L, yeast extract 20g / L, peptone 20g / L, magnesium sulfate 0.3g / L, disodium hydrogen phosphate 0.21g / L, sodium dihydrogen phosphate 0.2g / L, pH7.0, Sterilize the enzyme culture medium at 121°C for 15 minutes;

[0060] Pour the expanded strain culture solution obtained in step (2) into a 15-liter fermenter filled with enzyme-pr...

Embodiment 3

[0067] An application of bacillus circulans in the production of galacto-oligosaccharides by symbiotic fermentation technology, comprising the steps of:

[0068] (1) Bacteria expansion culture

[0069] Inoculate the slant of Bacillus circulans BLB-32 (CGMCC No.8864) in the seed medium of the shake flask, and cultivate it at 37°C for 24 hours at a shaking speed of 100rpm to obtain the expanded strain culture medium; seed medium: glucose 10g / L, yeast extract 10g / L, peptone 10g / L, magnesium sulfate 0.3 g / L, pH 7.0, the seed medium was sterilized at 121°C for 15 minutes;

[0070] (2) Enlarged culture for enzyme production

[0071] Enzyme production medium: lactose 10g / L, yeast extract 10g / L, peptone 10g / L, magnesium sulfate 0.3g / L, disodium hydrogen phosphate 0.2g / L, sodium dihydrogen phosphate 0.2g / L, pH7.0, Sterilize the enzyme culture medium at 121°C for 15 minutes;

[0072] Pour the expanded strain culture solution obtained in step (2) into a 15-liter fermenter filled with...

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Abstract

The invention relates to bacillus circulans and an application thereof in preparation of galactooligosaccharide by a symbiotic fermentation technology. The bacillus circulans BLB-32 has been already stored in the China General Microbiological Culture Collection Center on 27th, February, 2014 with the preservation number of CGMCCNo. 8864. The enzyme yielding activity of the bacillus circulans strain reaches up to over 150 mu / ml. Galactooligosaccharide is produced by conversion through the symbiotic fermentation technology, so that the purity in one pot for reaction is greater than 57%. By replacing the conventional technology of enzyme reaction and fermenting reaction, a series of high purity (greater than 90%) products with different galactooligosaccharide contents can be obtained through chromatograph of a galactooligosaccharide reaction solution obtained. According to the technology, investments on a thallus crusher, a centrifugal machine and supporting facilities can be saved, the occupied area can be saved, and a lot of energy consumption can be reduced. Meanwhile, the prepared enzyme is fully utilized, the loss is reduced, the enzyme utilization ratio is enhanced by over 10% and the utilization ratio of raw materials is improved.

Description

technical field [0001] The invention relates to the technical field of galacto-oligosaccharide preparation, in particular to a strain of Bacillus circulans, and also relates to the application of the Bacillus circulans strain in the preparation of galacto-oligosaccharides by symbiotic fermentation technology. Background technique [0002] Galacto-oligosaccharides (GOS for short) is a functional oligosaccharide with natural properties, which is an oligosaccharide with a polymerization degree of 2-8 composed of galactose and glucose groups. This technology uses lactose as raw material, uses β-galactosidase produced by Bacillus circulans to catalyze the hydrolysis of galactosidic bonds to generate galactose and glucose, and generates galactooligomeric di Galacto-oligosaccharide products from sugar to galacto-oligo-octasaccharide, and use symbiotic fermentation technology and chromatographic separation technology to produce high-purity galacto-oligosaccharides. [0003] Galac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/14C12P19/04C12P19/12C12R1/09
Inventor 刘宗利王乃强栾庆民张莉熊小兰周文福杜倩
Owner BAOLINGBAO BIOLOGY
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