A method for repairing rare earth contaminated soil with immobilized mixed bacterial agent
A mixed bacterial agent and rare earth pollution technology, applied in the field of remediation of rare earth polluted soil, can solve the problems of long growth cycle of hyperaccumulation plants, large changes in original soil, removal of pollution sources, etc. low impact effect
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[0035] (1) Preparation of strain mother liquor
[0036] Medium (g / L): Glucose 10.0g, KH 2 PO 4 0.2g, MgSO 4 ·7H 2 O0.2g, NaCl3g, CaSO 4 2H 2 O0.2g, CaCO 3 5.0g, beef extract 3g, peptone 5g,
[0037] Deionized water 1000mL, pH7.2, sterilized at 121°C for 20min
[0038] Enrichment medium containing rare earth ions: add 1.5-2ml of rare earth solution to the above medium, so that the total concentration of rare earth ions is 200-300mg / L.
[0039]Inoculate the strains in the enrichment medium containing rare earth ions at a ratio of 2:1 by weight, add 10 g of rare earth contaminated soil to the above medium, and culture in a constant temperature shaker at 28-30°C and 160 rpm / min for 5-7 days ,stand-by.
[0040] (2) Preparation of primary seed solution
[0041] Inorganic medium: NaCl1g, NH 4 SO 4 1g, K 2 HPO 4 1g, MgSO 4 ·7H 2 O0.1g,
[0042] 1000mL deionized water, pH7.2, sterilized at 121°C for 20min
[0043] Inoculate the above strain mother solution into a 250m...
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