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A method for repairing rare earth contaminated soil with immobilized mixed bacterial agent

A mixed bacterial agent and rare earth pollution technology, applied in the field of remediation of rare earth polluted soil, can solve the problems of long growth cycle of hyperaccumulation plants, large changes in original soil, removal of pollution sources, etc. low impact effect

Inactive Publication Date: 2016-03-09
INNER MONGOLIA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Different restoration technologies have their own advantages and disadvantages. The physical restoration method has the shortest period, the fastest effect, and the most thorough restoration, but the original soil changes greatly, requiring a large amount of engineering and high cost; chemical restoration has obvious effects and can maintain However, chemical remediation cannot remove pollution sources from the soil, and the use of chemical reagents may cause secondary pollution to the environment; phytoremediation is relatively low-carbon, environmentally friendly, and environmentally friendly, and it is not harmful to the original site. Cause damage, but hyperaccumulative plants have a long growth cycle and require multiple crops; as an emerging repair method, microorganisms are characterized by low cost and environmental friendliness, but the survival and growth of microorganisms are greatly affected by the environment, and are limited in the application process

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0035] (1) Preparation of strain mother liquor

[0036] Medium (g / L): Glucose 10.0g, KH 2 PO 4 0.2g, MgSO 4 ·7H 2 O0.2g, NaCl3g, CaSO 4 2H 2 O0.2g, CaCO 3 5.0g, beef extract 3g, peptone 5g,

[0037] Deionized water 1000mL, pH7.2, sterilized at 121°C for 20min

[0038] Enrichment medium containing rare earth ions: add 1.5-2ml of rare earth solution to the above medium, so that the total concentration of rare earth ions is 200-300mg / L.

[0039]Inoculate the strains in the enrichment medium containing rare earth ions at a ratio of 2:1 by weight, add 10 g of rare earth contaminated soil to the above medium, and culture in a constant temperature shaker at 28-30°C and 160 rpm / min for 5-7 days ,stand-by.

[0040] (2) Preparation of primary seed solution

[0041] Inorganic medium: NaCl1g, NH 4 SO 4 1g, K 2 HPO 4 1g, MgSO 4 ·7H 2 O0.1g,

[0042] 1000mL deionized water, pH7.2, sterilized at 121°C for 20min

[0043] Inoculate the above strain mother solution into a 250m...

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Abstract

The invention relates to a restoration technology for rare earth pollution, in particular to an immobilized mixed bacterial agent for repairing rare earth contaminated soil and a preparation method thereof. In this method, Bacillus megaterium and Bacillus colloidus are selected as the mixed strains for remediating rare earth-contaminated soil. The immobilization technology can keep the activity of the mixed strains for a long time, and enable them to stably exert high-efficiency performance in complex environments. The two bacteria selected in this technology can not only efficiently adsorb rare earth ions, but also decompose potassium, decompose phosphorus, fix nitrogen, increase soil fertility, prevent soil compaction, and prevent rapid evaporation of soil moisture. The by-products of crops are used as the immobilization carrier, which has wide sources, low cost and simple operation. It can not only be used as "waste", but also can be used as a biological fertilizer to provide various nutrients for plants. This technology can not only effectively repair rare earth contaminated soil, but also make the repaired soil more suitable for the growth of plants, etc.

Description

technical field [0001] The invention relates to a technology for repairing rare earth polluted soil, in particular to a technology and a preparation method for repairing rare earth polluted soil with an immobilized mixed bacterial agent. Background technique [0002] China is the country with the richest rare earth resources in the world, ranking first in the world in resource reserves, rare earth production, rare earth sales and rare earth consumption. In recent years, with the rapid development of science and technology and economy, especially the rapid development of various high-precision materials, the human demand for rare earth minerals is increasing, and the pace of development of rare earth minerals is gradually increasing. Only Baotou City In the past 10 years, the number of rare earth enterprises has increased by about 20. [0003] With the mining of rare earth deposits, a certain degree of rare earth pollution has been caused in rare earth mining and smelting ar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B09C1/10C12N11/02C12R1/11C12R1/07
Inventor 郑春丽张雪峰陈敏洁聂立王建英王亚琴程昊辰
Owner INNER MONGOLIA UNIV OF SCI & TECH