Silk fibroin freeze-dried powder with large molecular weight

Abstract

The invention discloses silk fibroin freeze-dried powder with large molecular weight. The silk fibroin freeze-dried powder is characterized in that the freeze-dried powder is powder obtained by performing degumming, lithium bromide-dissolving, dialyzing, centrifuging, high-temperature and high-pressure sterilizing and freeze-drying onto silks. The freeze-dried powder comprises stable nano-particles consisting of random coils and alpha-helixes. The silk fibroin freeze-dried powder disclosed by the invention has beneficial effects as follows: random coil molecular structure of the silk fibroin is kept in a freezing and drying process, so that the silk fibroin is not transformed towards a beta-folding direction or degree of the transformation is controlled, and therefore, silk fibroin molecules are prevented from interaction and accumulative crystallization. The silk fibroin freeze-dried powder disclosed by the invention is the silk fibroin freeze-dried powder with large molecular weight, which can be quickly and completely dissolved in water, and is applicable to a plurality of biomedical and clinical fields.

Description

technical field [0001] The invention relates to the field of biological materials, in particular to a large molecular weight silk fibroin freeze-dried powder. Background technique [0002] In recent years, starting from the regenerated silk fibroin solution, a variety of degradable biomaterials such as thin films, particles, porous scaffolds and hydrogels after further processing have been proved to have excellent biocompatibility and non-immunogenicity. It degrades slowly and is becoming a new type of medical biomaterial. It can be directly used as a product for tissue repair in the clinic, and can also be used as a drug and cell carrier scaffold for drug sustained release and tissue engineering to build artificial organs. The molecular weight of natural silk fibroin in silkworm cocoons is about 3 million Daltons. After acid-base treatment, silk fibroin is degraded into small molecular weight (< 100,000 Daltons) polypeptides, which exist in the form of random coils. Alt...

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Problems solved by technology

[0003] The existing large-molecular-weight silk fibroin solution (regenerated silk fibroin solution) is difficult to store and transport after purification, and it is necessary to sell the regenerated silk fibroin solution directly as a commodity or as the main component of an injectable gel. Consider the problem of storage temperature and conditions, which is also the primary problem restricting its large-scale applic

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