Silk fibroin freeze-dried powder with large molecular weight

Abstract

The invention discloses silk fibroin freeze-dried powder with large molecular weight. The silk fibroin freeze-dried powder is characterized in that the freeze-dried powder is powder obtained by performing degumming, lithium bromide-dissolving, dialyzing, centrifuging, high-temperature and high-pressure sterilizing and freeze-drying onto silks. The freeze-dried powder comprises stable nano-particles consisting of random coils and alpha-helixes. The silk fibroin freeze-dried powder disclosed by the invention has beneficial effects as follows: random coil molecular structure of the silk fibroin is kept in a freezing and drying process, so that the silk fibroin is not transformed towards a beta-folding direction or degree of the transformation is controlled, and therefore, silk fibroin molecules are prevented from interaction and accumulative crystallization. The silk fibroin freeze-dried powder disclosed by the invention is the silk fibroin freeze-dried powder with large molecular weight, which can be quickly and completely dissolved in water, and is applicable to a plurality of biomedical and clinical fields.

Description

technical field [0001] The invention relates to the field of biological materials, in particular to a large molecular weight silk fibroin freeze-dried powder. Background technique [0002] In recent years, starting from the regenerated silk fibroin solution, a variety of degradable biomaterials such as thin films, particles, porous scaffolds and hydrogels after further processing have been proved to have excellent biocompatibility and non-immunogenicity. It degrades slowly and is becoming a new type of medical biomaterial. It can be directly used as a product for tissue repair in the clinic, and can also be used as a drug and cell carrier scaffold for drug sustained release and tissue engineering to build artificial organs. The molecular weight of natural silk fibroin in silkworm cocoons is about 3 million Daltons. After acid-base treatment, silk fibroin is degraded into small molecular weight (< 100,000 Daltons) polypeptides, which exist in the form of random coils. Alt...

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Problems solved by technology

[0003] The existing large-molecular-weight silk fibroin solution (regenerated silk fibroin solution) is difficult to store and transport after purification, and it is necessary to sell the regenerated silk fibroin solution directly as a commodity or as the main component of an injectable gel. Consider the problem of storage temperature and conditions, which is also the primary problem restricting its large-scale application development

In addition, the regenerated silk fibroin solution required for the preparation and application of certain materials has a high concentration, and the purified silk fibroin solution can only be obtained by further concentration methods, such as polyethylene glycol dialysis, ultrafiltration, etc. Concentration silk fibroin solution, bringing additional cost and contamination risk to material preparation

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