Method for effectively producing protocorm by using zamiolulcas zamiif olia compound leaf rachis

A technology of snow iron taro and protocorm, which is applied in the field of high-efficiency production of protocorm, can solve the problems of slow growth of cultivated seedlings, failure to meet the requirements of commercial seedlings on the market, and different cultivation purposes, and achieve easy storage, long-distance transportation, and convenient transportation Effect

Active Publication Date: 2014-07-23
CHANGCHUN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Second, the purpose of training is different
According to the research and experiment of the project team, the tissue cultured seedlings grow slowly, and after two years of cultivation, they still cannot meet the market requirements of commercial seedlings
Therefore, this technology has not been promoted and used in production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Step 1: Pre-cultivation of snow iron taro

[0032] Put the snow iron taro planted in the flower pot in a room fumigated and disinfected with potassium permanganate and formaldehyde, cover the plant with a black plastic bag to promote the stems and leaves to grow leggy, and after 30 days, cut the leggy snow iron taro Taro compound leaves are reserved.

[0033] Step 2: Prepare culture medium

[0034] The corn cobs are crushed into 5-10 mm particles with a pulverizer, and sterilized at 121° C. for 17 minutes in a high-pressure steam sterilizer. Take a tissue culture bottle with a volume of 200 ml, and add sterilized corncob particles 15 to 20 mm high in each bottle.

[0035] Dryopteris crassirhizoma Nakai was crushed into juice with a tissue breaker and left to stand for 10 minutes, and the supernatant was taken for later use.

[0036] For every 1000 milliliters of distilled water, add 150 milliliters of supernatant of Trichophyllum chrysogenum, 6 grams of agar, 20 gram...

Embodiment 2

[0043] Step 1: Pre-cultivation of snow iron taro

[0044] Put the snow iron taro planted in the flower pot in a room fumigated and disinfected with potassium permanganate and formaldehyde, cover the plant with a black plastic bag to promote the stems and leaves to grow leggy, and after 30 days, cut the leggy snow iron taro Taro compound leaves are reserved.

[0045] Step 2: Prepare culture medium

[0046] The corn cobs are crushed into 5-10 mm particles with a pulverizer, and sterilized at 121° C. for 15 minutes in a high-pressure steam sterilizer. Take a tissue culture bottle with a volume of 200 milliliters, and add sterilized corn cob particles to a height of 15 millimeters into each bottle.

[0047] Dryopteris crassirhizoma Nakai was crushed into juice with a tissue breaker and left to stand for 20 minutes, and the supernatant was taken for later use.

[0048] For every 1000 milliliters of distilled water, add 200 milliliters of supernatant of Trichophyllum chrysogenum,...

Embodiment 3

[0055] Step 1: Pre-cultivation of snow iron taro

[0056] Put the snow iron taro planted in the flower pot in a room fumigated and disinfected with potassium permanganate and formaldehyde, cover the plant with a black plastic bag to promote the stems and leaves to grow leggy, and after 30 days, cut the leggy snow iron taro Taro compound leaves are reserved.

[0057] Step 2: Prepare culture medium

[0058] The corn cobs are crushed into 5-10 mm particles with a pulverizer, and sterilized at 121° C. for 20 minutes in a high-pressure steam sterilizer. Take a tissue culture bottle with a volume of 200 milliliters, and add sterilized corn cob particles to a height of 20 millimeters into each bottle.

[0059] Dryopteris crassirhizoma Nakai was crushed into juice with a tissue breaker and left to stand for 15 minutes, and the supernatant was taken for later use.

[0060] For every 1000 milliliters of distilled water, add 100 milliliters of supernatant of Trichophyllum chrysogenum,...

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PUM

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Abstract

The invention relates to a method for effectively producing a protocorm by using a zamiolulcas zamiif olia compound leaf rachis, and belongs to the technical field of flower culture. The method comprises pre-culture of zamiolulcas zamiif olia, preparation of a culture medium, inoculation and culture of the protocorm. In the method, the compound leaf rachis is taken as a plant propagation material, and the cultured protocorm can be taken as a subculture material without shearing the stems or leaves of zamiolulcas zamiif olia once again. By adopting the method, the propagation coefficient of zamiolulcas zamiif olia is increased by over 100 times, the propagation period is shortened to one third of leaf cutting propagation, and the demand of a large number of excellent seedlings in production can be met; the protocorm is taken as a target propagule, and has the advantages of easiness in storage, convenience for long-distance transportation and reduction in spreading of diseases and insect pests during transportation; by taking corn cob granules as a culture medium, the production cost is reduced, and recycling of agricultural waste is promoted; at callus induction and protocorm induction stages, researches of breed improvement and new variety breeding can be performed in combination with modern biotechnologies.

Description

technical field [0001] The invention belongs to the technical field of flower cultivation, and in particular relates to a method for efficiently producing protocorms by utilizing the compound leaf axis of citrus taro. Background technique [0002] Zamiolulcas zamiif olia (Zamiolulcas zamiif olia) is a perennial evergreen herbaceous plant of the genus Zamiolulcas in the Araceae family. Because its leaflets are arranged in an even-numbered pinnate shape on the axis of the compound leaves, the leaves are thick and bright, like a string connected in series. Coins imply wealth and wealth, auspiciousness and wealth, so they are also called money trees. [0003] At present, the sub-root and leaf cutting propagation methods used in the production of snow iron taro have low reproduction coefficient and long cycle, which are far from meeting the market demand. Moreover, the research group conducted leaf cutting propagation experiments and found that the leaf cutting propagation method...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 马艳丽丁云峰武术杰周秀华高巍尹立辉谢修鸿王晓红杨义波
Owner CHANGCHUN UNIV
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