A kind of bacillus cereus bacterial strain and application

A technology of Bacillus cereus, which is used in the field of microorganisms, can solve problems such as damage and microbial infection, limit the development of the strawberry industry, and affect the interests of producers, and achieve strong tolerance, easy cultivation and preservation, and reliable sources. Effect

Inactive Publication Date: 2016-07-06
INST OF PLANT PROTECTION & SOIL FERTILIZER HUBEI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002]Strawberry is rich in nutrition and is known as the "Queen of Fruits". Damage and microbial infestation cause it to rot and deteriorate
Among them, gray mold caused by Botrytis cinerea Persex Fr is one of the most important diseases in strawberry production. When it is serious, it can cause the loss of strawberry yield to reach more than 50%, which directly affects the interests of producers and also limits the development of strawberry industry. Further development

Method used

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  • A kind of bacillus cereus bacterial strain and application
  • A kind of bacillus cereus bacterial strain and application
  • A kind of bacillus cereus bacterial strain and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1: Isolation of Bacillus cereus zy-1 of the present invention

[0015] Isolation and purification of antagonistic bacteria: ①Weigh 10g wheat field soil sample taken from Zaoyang City, Hubei Province, ②Place it in 200mL sodium hexametaphosphate solution (dispersant), shake and suspend for 5min, ③In a suspended state, absorb Add 20mL to 80mL of 0.1% water agar, shake and suspend for 5 minutes, ④When the suspension is in a stirring state, absorb 0.3mL of the suspension, ⑤Dilute the 0.3mL suspension by 10 times, respectively -1 , 10 -2 And 10 -3 , ⑥ Place the gradient dilutions in an oven at 80°C for 30 min, take them out and wait to cool down, ⑦ spread 3 plates (3 plates for each dilution concentration), 1 / 10 TSA selective medium, ⑧ All plates were cultured at 22°C, and single colonies were selected after 2-3 days and stored with numbers. The inhibitory effect on Botrytis cinerea was tested by confronting culture in a plate, and the strain that formed the inhibition z...

Embodiment 2

[0016] Example 2: Determination of the Physiological and Biochemical and 16SrDNA Sequence of Bacillus cereus of the present invention

[0017] Physiological and biochemical test results: zy-1 cultured on NA plate medium at 28°C for 36 hours, the colonies are nearly round, off-white, opaque, large colonies, 3-7mm, irregular edges and diffused, rough particles on the surface like ground glass or Waxy, does not secrete pigment; is a gram-positive bacteria, rod-shaped, 1.0~1.3×3.0~5.0μm, chain, motility, spore oval, terminal or subterminal, spore expansion is not obvious; Cultured by 7% NaCl, contact enzyme positive, glucose fermentation positive, starch hydrolysis positive, nitrate reduction positive, gelatin liquefaction positive, maltose fermentation positive, semi-solid puncture test positive, casein hydrolysis positive, VP test negative, citrate Utilization test is negative, indole test is negative, sucrose fermentation is negative, lactose fermentation is negative, mannitol hyd...

Embodiment 3

[0020] Example 3: Test of Bacillus cereus of the present invention on spore germination and hypha growth of Botrytis cinerea

[0021] 1. Preparation of Bacillus cereus culture filtrate: After strain zy-1 was shaken cultured at 28°C for 3 days, the fermentation broth was centrifuged (12000r / min, 20min), and the supernatant was sterilized with a bacterial filter (0.22μm) to obtain The sterile fermentation broth.

[0022] The spore germination test was carried out by the concave slide method. The Strawberry Botrytis cinerea was attached to a PDA plate and cultured at 23°C for 5 days. After the sporulation, the spores were eluted with sterile water to make a concentration of 10 5 Each / ml spore suspension, suck 50μL of sterile fermentation broth and add it to a 500μL centrifuge tube, and then suck 50μL of the prepared spore suspension to mix the medicine and the spore suspension evenly. Pipette 40 μL of the above mixed solution and drop it on the concave glass slide, then place it in a...

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Abstract

The invention discloses a bacillus cereus bacterial strain (Bacilluscereuszy-1) and applications thereof. The bacillus cereus bacterial strain (Bacilluscereuszy-1) is preserved in China Center for Type Culture Collection (Wuhan university, Wuhan, China), and preservation number is No.M2014007. Germination inhibition rate of a 2-fold diluent of a fermentation aseptic filtrate of Bacilluscereuszy-1 on botrytis cinerea spores is 95.2%, and inhibition rate of a 10-fold diluent of the fermentation aseptic filtrate of Bacilluscereuszy-1 on mycelia growth is higher than that of a 40% pyrimethanil suspension, so that application prospect of the bacillus cereus bacterial strain (Bacilluscereuszy-1) in agriculture is promising. Technical route of the bacillus cereus bacterial strain (Bacilluscereuszy-1) is reasonable, reliable pilotscale experiment data and design consideration are provided for large-scale industrialized production; and establishment of foundation is provided for development of related biological products and field promotion and application.

Description

Technical field [0001] The invention belongs to the technical field of microorganisms, and relates to the application of bacteria in the field of biocontrol, and in particular to the application of Bacillus cereus strains in preventing and treating strawberry gray mold. Background technique [0002] Strawberry is rich in nutrients and is known as the "Queen of Fruits". However, due to its delicate and juicy tissue and thin skin, it is vulnerable to damage and microbial infection during harvesting, storage and transportation, causing it to rot and deteriorate. Among them, the gray mold disease caused by Botrytis cinerea PersexFr is one of the most important diseases in strawberry production. In severe cases, it can cause the loss of strawberry production by more than 50%, which directly affects the interests of producers and limits the strawberry industry. Further development. For a long time, the most effective method to control strawberry gray mold is still to use chemical fung...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01P3/00C12R1/085
Inventor 喻大昭龚双军向礼波杨立军史文琦张学江曾凡松薛敏峰汪华
Owner INST OF PLANT PROTECTION & SOIL FERTILIZER HUBEI ACAD OF AGRI SCI
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