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A test strip and method for detecting sulfonamides and fluoroquinolones

A technology for sulfonamides and fluoroquinolones, which is applied in the field of test strips for detecting sulfonamides and fluoroquinolones, can solve the problem that the method for simultaneous detection of multiple antibiotics is less researched, and is not suitable for large-scale sample screening and on-site The detection and processing process is complicated, and the detection time is short, easy to popularize and use, and the operation is simple.

Active Publication Date: 2016-08-31
BEIJING KWINBON BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the large difference in the properties of veterinary drugs, the existing residue analysis generally establishes corresponding detection methods according to the same family of drugs with similar chemical structures. There are few researches on the method, which cannot meet the needs of the increasing variety of veterinary drugs and the continuous improvement of the analysis throughput.
At present, there are also researches on liquid chromatography-mass spectrometry technology. This method has high sensitivity, good selectivity and specificity, and can accurately confirm the qualitative confirmation of low-concentration samples. However, the sample pretreatment process is complicated and the degree of instrumentation is high. And the price is expensive, the analysis speed is slow, it is only suitable for the confirmation analysis of samples, and it is not suitable for the screening and on-site detection of large quantities of samples

Method used

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  • A test strip and method for detecting sulfonamides and fluoroquinolones
  • A test strip and method for detecting sulfonamides and fluoroquinolones
  • A test strip and method for detecting sulfonamides and fluoroquinolones

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1 detects the composition of the test strip of sulfonamides and fluoroquinolones

[0044] 1. Test paper ( figure 1 )

[0045] The test paper is composed of a bottom plate, a sample absorption pad, a reaction film, a water absorption pad, and a protective film;

[0046] The sample absorbent pad 1, the reaction film 2, the water absorbent pad 3 and the protective film 7 are pasted on the bottom plate 6 in sequence, the end of the sample absorbent pad is connected with the reaction film, the end of the reaction film is connected with the water absorbent pad, and the sample absorbent pad The beginning is aligned with the beginning of the bottom plate, and the end of the absorbent pad is aligned with the end of the bottom plate;

[0047] The test paper is pasted with a protective film, and the protective film 7 is covered on the sample absorption pad, which is the detection end, and the word MAX is printed on it ( figure 2 );

[0048] There are detection lin...

Embodiment 2 Embodiment 1

[0053] The preparation method of test strip described in embodiment 2 embodiment 1

[0054] 1. Preparation of test strips

[0055] The preparation method of this test strip mainly comprises the following steps:

[0056] 1) Prepare freeze-dried microporous reagents with sulfa drug monoclonal antibody-colloidal gold marker and fluoroquinolone drug monoclonal antibody-colloidal gold marker;

[0057] 2) Prepare two detection lines coated with sulfonamide drug hapten-carrier protein conjugates and fluoroquinolone drug hapten-carrier protein conjugates and a quality control line coated with goat anti-mouse anti-antibodies the reaction membrane;

[0058] 3) Assemble the reaction film prepared in 2) with the sample absorbent pad, water absorbent pad, protective film, and bottom plate into a test paper;

[0059] 4) Assemble the freeze-dried microporous reagents prepared in 1) and 3) with the sulfa drug monoclonal antibody-colloidal gold marker and the fluoroquinolone drug monoclonal...

Embodiment 3

[0111] The detection of sulfonamides and fluoroquinolones in embodiment 3 milk

[0112] 1. Test with test strips

[0113] Take out the required number of microwell reagents and test papers from the original package (pre-equilibrated to room temperature if stored at low temperature), and mark them; use a micropipette to draw 200 μl of the milk solution to be tested into the microwell, slowly suck and Fully mix with the reagents in the microwells; insert the marked test paper into the microwells—the end of the printed "MAX" line faces down, so that it is fully immersed in the solution; incubate at room temperature (20-25°C) for 5 minutes, take out the test paper, judgement result.

[0114] 3. Analysis of test results

[0115] Negative: When the quality control line (C) shows a red band, the detection line (T 1 ) and detection line (T 2 ) also show a red band at the same time, judged to be negative, such as Figure 4 as shown in a;

[0116] Positive for sulfa drugs: when the...

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Abstract

The invention discloses a dipstick used for testing sulfonamides and fluoroquinolones and test method thereof. The dipstick consists of a dipstick body and a microporous reagent, the said microporous reagent contains a freeze-dried sulfonamides monoclonal antibody-colloidal gold marker and a freeze-dried fluoroquinolones monoclonal antibody-colloidal gold marker; the said dipstick body consists of a sample absorption pad, a reaction film, an absorbent pad, a protective film and a bottom patch which are connected in sequence, the said reaction film includes two detection lines and a quality control line, the said two detection lines are respectively coated with sulfonamides hapten carrier protein conjugates and fluoroquinolones hapten carrier protein conjugates, the said quality control line is coated with goat-anti-mouse antibody. Using the dipstick introduced in this invention to test sulfonamides and fluoroquinolones is convenient, fast, intuitive, and accurate. The dipstick is convenient to carry, is wide in application range and low in cost and is easy to promote and use.

Description

technical field [0001] The invention relates to a test strip and a method for detecting sulfonamides and fluoroquinolones, in particular to a colloidal gold test strip for simultaneously detecting sulfonamides and fluoroquinolones in milk. technical background [0002] Sulfonamides (SAs) are a class of chemotherapeutic drugs with the structure of sulfanilamide, which inhibit the reproduction of bacteria by affecting the synthesis of nuclear proteins. Fluoroquinolones (Fluoroquinolones, FQs) are synthetic antibacterial drugs containing 4-quinolone basic structure, which achieve antibacterial effect by inhibiting the synthesis of bacterial DNA. Because FQs and SAs can inhibit most Gram-positive and negative bacteria, are stable in nature, low in price, and easy to use, drugs with subtherapeutic concentrations are often used as feed additives to prevent the occurrence of diseases, improve the conversion rate of feed, and promote animal health. grow. However, adverse reactions...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577G01N33/04
CPCG01N33/558G01N33/9446
Inventor 何方洋冯才伟田甜冯月君崔海峰余厚美徐念琴
Owner BEIJING KWINBON BIOTECH
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