Bacillus ES2-45 capable of reducing selenite to produce nano-selenium and application thereof

A bacillus and selenium-enriched technology, applied in the field of environmental microorganisms, can solve problems such as high toxicity, and achieve the effects of low cost, high anti-environmental viability, and environmental friendliness

Active Publication Date: 2014-07-30
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, selenite is generally added to the feed, but it is highly toxic

Method used

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  • Bacillus ES2-45 capable of reducing selenite to produce nano-selenium and application thereof
  • Bacillus ES2-45 capable of reducing selenite to produce nano-selenium and application thereof
  • Bacillus ES2-45 capable of reducing selenite to produce nano-selenium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] A kind of acquisition of selenite reducing bacterial strain ES2-45, its screening process is as follows:

[0046] A. Utilize the characteristic that the bacterial strain can reduce colorless selenite into red nano-selenium, thereby making the colony red. Sampling from a characteristic selenium ore soil in Enshi Autonomous Prefecture, Hubei Province, the "Selenium Capital of China", weighed 10g of ore soil solution In 90mL sterile water, gradually dilute to 10 -1 , 10 -2 up to 10 -5 Suspension, take the appropriate dilution and spread it on the ordinary solid bacterial culture medium 1 / 10TSB, add Na to the medium 2 SeO 3 The final concentration was 1 mmol / L, and cultured in a constant temperature incubator at 28°C for 7 days.

[0047] B. Select the reddened colony and streak it on a 1 / 10 TSB plate with a final selenite concentration of 1 mmol / L, confirm the reddened colony, record the morphology and store it.

[0048] C. Add 1mmol / L Na 2 SeO 3 The 1 / 10 TSB liquid ...

Embodiment 2

[0063] A fermentation method of selenite-reducing bacterial strain ES2-45, the steps of which are:

[0064] Connect a loop of bacteria from the activated plate into the liquid medium (1 / 10 TSB medium), cultivate it at 28°C and 160rpm for 24h, then inoculate it in 1 / 10TSB liquid medium according to the inoculum size of 5% to carry out expanded culture, 28 Cultivate at 160 rpm for 48 hours.

[0065] The formula of the 1 / 10 TSB liquid medium is: tryptone 1.5g / L, soybean peptone 0.5g / L, sodium chloride 0.5g / L, adjust the pH value to 7.3±0.2, sterilize at 115°C for 30min.

[0066] The formula of the culture medium of the plate is: add 1.5-2.0% agar to 1 / 10 TSB liquid culture medium.

Embodiment 3

[0068] Heavy Metal Resistance of Bacillus ES2-45

[0069] Collect the bacterial solution cultured for 24 hours, and spot 2 μL on 1 / 10 TSB agar plates containing different concentrations of different heavy metals by spotting, and observe its growth status. The concentration at which the strain does not grow is the MIC of the heavy metal. 45 has strong resistance to various heavy metals.

[0070] Table 2 Heavy Metal Resistance of Bacillus ES2-45

[0071]

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PUM

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Abstract

The invention discloses a bacillus ES2-45 capable of reducing selenite to produce nano-selenium and application thereof. The collection number of the bacillus ES2-45 is CCTCC (China Center For Type Culture Collection) NO:M2014094. The strain has the capability of fastly reducing toxic selenite into low-toxicity nano-selenium, red nano-selenium can be produced within 4h when the strain is cultured in 1 / 10TSB culture medium containing 1mmol / LNa2SeO3, the 1mmol / LNa2SeO3 can be reduced completely in 48h, the yield of nano-selenium is high up to 239, 290+ / -20.11mg / kg. Therefore, after being added into an animal feed, the nano-selenium and thalli produced by fermentation of the bacillus in the selenite-containing culture medium are safe and low-toxicity, easy to absorb, and capable of improving the quality of animal meat. The bacillus is easily fermented and stored and the strain is capable of efficiently reducing selenite, so that the strain has good application prospects on the aspect of microorganism treatment of the selenium-polluted soil.

Description

technical field [0001] The invention belongs to the technical field of environmental microbes, and relates to a bacillus ES2-45 strain capable of reducing selenite to red nano-selenium, and the application of the bacterium in adding nano-selenium to feed and bioremediation of selenium-polluted environments. Background technique [0002] Selenium (Selenium), element symbol Se, located in the main group VI of the periodic table of elements, is a non-metallic trace element necessary for life. In nature, selenium mainly exists in four valence states: reduced selenide (-2), elemental selenium (0), and oxidized selenite (+4) and selenate (+6). Among them, selenite (+4) is the most toxic, and elemental selenium (0) is the least toxic. [0003] Selenium is a component of the 21st amino acid (selenocysteine ​​Sec, U) and a component of the important peroxidase glutathione peroxidase in the body, so it is essential to human health. It plays an irreplaceable role in the antioxidant d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K1/16B09C1/10C12R1/07A23K10/18
Inventor 郑世学王革娇姚蓉
Owner HUAZHONG AGRI UNIV
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